In-vitro micronucleus detection method for electronic cigarette aerosol aqueous extract

Abstract

The invention discloses an in-vitro micronucleus detection method for an electronic cigarette aerosol aqueous extract. The method includes the steps of sample pretreatment, single cell suspension preparation, cell concentration calculation, cell inoculation, sample addition and test substance grouping, tested substance incubation, cell harvesting, low permeation, slide dropping, staining, micronucleus observation, result judgment and the like. Use characteristics and an action mode of electronic cigarette products are comprehensively investigated, a brand-new sample pretreatment method is established, human pulmonary fibroblasts HPF are selected as cells for detection, and the sample detection dosage is determined. With the effective treatment of the sample, the optimal setting of the detection dosage and the correct selection of target cells, the method can accurately and effectively detect the influence degree of the electronic cigarette aerosol aqueous extract on cell micronucleus rates, and then the genotoxicity of the electronic cigarette products is determined.

Description

technical field [0001] The invention belongs to the technical field of safety biological evaluation of tobacco and tobacco products, and specifically relates to a method for detecting the influence degree of the aerosol aqueous extract of electronic cigarette smoke on the rate of cell micronuclei, and then determining its genotoxicity . Background technique [0002] Micronucleus test has a clear detection end point, simple method, and easy to carry out. It is one of the standard combination tests of genotoxicity. It has been widely used in detection and early forecasting. In 2009, the Organization for Economic Co-operation and Development (OECD) published a guidance document for in vitro micronucleus tests in mammals, initially establishing a unified standard for in vitro micronucleus tests in mammals. In 2002, CORESTA, the International Tobacco Scientific Research Cooperation Center, established a working group for in vitro toxicological testing of cigarette smoke. After ...

AI Technical Summary

Problems solved by technology

However, the existing technology usually directly analyzes and tests the e-cigarette liquid itself, without considering its shape, migration and other processes during smoking, resulting in that the results of the analysis and test cannot accurately reflect the real genotoxicity of e-cigarette smoke

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