Method for detecting influence of electronic cigarette product on micronucleus rate of cells

Abstract

The invention discloses a method for detecting the influence of an electronic cigarette product on the micronucleus rate of cells. The method comprises the following steps: preprocessing test samples, preparing a human lung fibroblast single cell suspension fluid, calculating the concentration of the human lung fibroblast single cell suspension fluid, inoculating the human lung fibroblast single cell suspension fluid, dividing the test samples into groups, setting the detection dosages of the test samples, preparing a test sample culture medium, subjecting the test sample culture medium to low permeability, and preparing a droplet slice of the test sample culture medium. The using characteristics and effect modes of electronic cigarette products are taken into account, an electronic cigarette product sample preprocessing method is established; according to the electronic cigarette product acted target cells, human lung fibroblast is taken as the cell for the cytotoxicity tests of electronic cigarette products, the sample detection dosage is determined at the same time, and thus an in-vitro micronucleus test method suitable for the electronic cigarette products is established.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and in particular relates to a method for detecting the influence of electronic cigarette products on the micronucleus rate of cells. Background technique [0002] Micronucleus test has a clear detection end point, simple method, and easy to carry out. It is one of the standard combination tests of genotoxicity. Damage detection and early forecasting have been widely used. In 2009, the Organization for Economic Co-operation and Development (OECD) published a guidance document for in vitro micronucleus tests in mammals, initially establishing a unified standard for in vitro micronucleus tests in mammals. In 2002, CORESTA, the International Tobacco Scientific Research Cooperation Center, established a working group for in vitro toxicological testing of cigarette smoke. After a large number of literature research and research work, the working group recommended the use of in vitro micronucl...

AI Technical Summary

Problems solved by technology

No smoke is produced during use, and its use method is significantly different from traditional cigarettes. Changes in the test object lead to differences in sample pretreatment methods, test cells, and test doses. In vitro micronucleus test for the detection of genotoxicity of traditional cigarettes It cannot meet the needs of genotoxicity testing of e-cigarette products, and domestic and foreign research institutions and tobacco companies have not yet formulated a standard method for in vitro micronucleus testing of e-cigarette products

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