Digital microfluidic chip and pathogen immunoassay method based thereon

Abstract

The invention relates to the technical field of microfluidic chips, in particular to a digital microfluidic chip and a pathogen immunoassay method based thereon. The method comprises the following steps of: (1) making a digital microfluidic chip; (2) modifying the antigen in a detection region of the digital microfluidic chip; and (3) adding the sample and running the digital microfluidic chip, photographing and detecting a fluorescent signal through a fluorescence detection system CCD and performing a quantitative analysis. The digital microfluidic chip comprises a sample hole, a reagent electrode forming a reagent reservoir, a transport electrode forming a droplet operation channel, and a detection electrode forming a detection region. The digital microfluidic chip and the pathogen immunoassay method based thereon have short reaction time, high detection sensitivity, simple peripheral equipment, high flexibility and low energy consumption, can meet the needs of community hospitals, remote clinics and the like, are suitable for building portable analytics platforms, can be analyzed in batches or in a single sample, and has strong adaptability to the sample size.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a digital microfluidic chip and a pathogen immune detection method based on it. Background technique [0002] Immunoassay is one of the most important detection methods in clinical testing at present. It is widely used in disease diagnosis, pathogen screening, drug detection, etc., and is also widely used in environmental monitoring, food safety and other fields. Since the 1980s, the introduction and improvement of automated detection technology has made automatic and high-throughput immunoassay possible, greatly promoting the application and promotion of immunoassay technology. [0003] The current automated immunoassay instruments have disadvantages such as cumbersome operation, slow response, long time consumption, large consumption of reagents and samples, and high detection cost. At the same time, the detection equipment has a complex structure, relies on high-pr...

AI Technical Summary

Problems solved by technology

[0003] The current automated immunoassay instruments have disadvantages such as cumbersome operation, slow response, long time consumption, large consumption of reagents and samples, and high detection cost.

At the same time, the detection equipment has a complex structure, relies on high-precision manufacturing technology, is expensive, and bulky, and requires trained professionals to operate

In addition, the current automated immunoassay instruments are mainly designed for batch testing, and most instruments only provide 2-3 emergency testing channels, which is poor in flexibility and not suitable for the detection of sporadic samples

With the introduction of concepts such as point-of-care, home health tests, and tele-diagnosis, as well as the development of community hospitals, the existing automated immune testing equipment can no longer meet the requirements The demand of modern medical development model

The test strip detection represented by colloidal gold technology is convenient to use, but its detection target is limited and the detection sensitivity is low, and the detection effect is limited.

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