123 results about "Steroid hormone" patented technology

A nanoscale molecularly imprinted polymer (MIP) thin film for small molecule detection, a method of manufacturing the same, and an electrochemical sensor using the MIP and quantum electrochemical impedance spectroscopy (EIS) are described. A plurality of specific recognition spaces for small molecules of 1000 Da or less are formed in the MIP, a receptor polymer is present at one end of the specific recognition spaces, a redox probe is present in a wire form, and the small molecule is a steroid hormone or a protein. The sensor is useful in point-of-care applications because it exhibits a rapid and reversible small molecule detection ability through a simple electrochemical regeneration process without cumbersome washing and solution replacement steps in the manufacturing process, thereby enabling continuous detection.

The invention discloses interference engineering bacteria for blocking early infection of animal parasitic nematodes and application, and belongs to the field of animal parasitic disease prevention and treatment. According to the interference engineering bacteria disclosed by the invention, the HCON00038080 (cyp-22a1) gene of haemonchus contortus is specifically silenced by expressing double-stranded RNA (dsRNA), so that the biosynthesis of endogenous bile acid-like steroid hormone of the haemonchus contortus is inhibited, the activation of infectious larvae is influenced, and the infection establishment process of the infectious larvae is blocked. The invention can be used for preventing early infection of haemonchus disease of ruminants such as cattle, sheep, camels and the like and the phenomenon of spring / perinatal high tide, has higher application value for prevention and control of haemonchus disease, and has reference value for prevention and control of parasitic nematode disease of other animals.

The invention belongs to the technical field of steroid hormone preparation, and particularly relates to a method for dehydrogenating Anecorstat Acetate, the Anecorstat Acetate is used as a substrate, Nocardioides simplex is used as a fermentation strain, biological fermentation conversion is carried out to obtain a product C, and the structural formula of the product C is # imgabs0 #; during the biological fermentation conversion, Tween 80, methanol and PPE are also added, the weight ratio of the Anekostat acetate to the Tween 80 is 200: (0.2-2), the weight ratio of the Anekostat acetate to the methanol is 200: (10-30), and the weight ratio of the Anekostat acetate to the PPE is 200: (10-50); the yield and the purity are improved, and meanwhile the production cost is reduced.

Compounds of formula (I), pharmaceutical compositions thereof, and kits thereof, are disclosed wherein A1 is C(O) or CHRz1 with Rz1 being OH, amine, imine, halogen, (C1-C5)alkoxy, OC(O)(C1-C5)alkyl, or OSO2-amine; A2 is CH2 or O; R1 is hydrogen, (C1-C5)alkyl optionally substituted with one or more Z substituents, (C1-C5)alkoxy optionally substituted with one or more Z substituents, (C2-C5)alkenyl optionally substituted with one or more Z substituents, (C2-C5)alkynyl optionally substituted with one or more Z substituents, (C3-C8)cycloalkyl, aryl, or heteroaryl; Z is halogen, OH, (C1-C5)alkyl, (C1-C5)haloalkyl, (C1-C5)alkoxy, (C1-C5)haloalkoxy, or amine; R2 is a heterocyclic aromatic ring having 5, 6 or 7 members having at least one N, S and / or O; R3 is a (C1-C5)alkyl substituted with one or more Br, Br76; I, I123, I124, or I131; m is an integer from 0 to 2; n is an integer from 0 to 2; and p is an integer from 0 to 5. Compounds of Formula I are inhibitors of 17β-Hydroxysteroid dehydrogenase type 1 (17β-HSD1) and are for use in the treatment or prevention of steroid hormone dependent disease, such as breast cancer, lung cancer, prostate carcinoma, ovarian cancer, uterine cancer, endometrial cancer and endometrial hyperplasia.

The invention provides a functional peptide with a yang tonifying effect, a seal penis peptide and a preparation method and application of the functional peptide and the seal penis peptide. The functional peptide comprises a peptide fragment pER and can promote testicular interstitial cells to secrete testosterone. Testosterone is steroid hormone and can be secreted by testicular interstitial cells through enzymatic reaction. The low testosterone level in serum can cause male testicular atrophy and low sperm survival rate, so that the male reproductive function is influenced, and the phenomena of poor sexual desire, premature ejaculation, erectile dysfunction and the like can also be caused. In addition, the testosterone also has the effects of maintaining muscle strength and quality, maintaining bone density and strength, refreshing, improving physical ability and the like. Therefore, the functional peptide provided by the invention has the effect of promoting the secretion of testosterone, which means that the functional peptide not only can effectively improve the male sexual function, but also can alleviate the damage of male sexual dysfunction to the body and spirit.

The invention discloses a steroid hormone degrading bacterium as well as a screening method and application thereof, and relates to the field of biological medicines, the steroid hormone degrading bacterium comprises klebsiella pneumoniae (KP2022 Klebsiella pneumoniae) with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2025478. The steroid hormone degrading bacterium provided by the invention has the capability of efficiently degrading dexamethasone, is beneficial to degrading dexamethasone in the environment, is conveniently obtained by adopting a screening method, and provides a sample for research on degradation of steroid hormones.

The invention discloses an antioxidant nano material as well as a preparation method and application thereof, and belongs to the technical field of material preparation. On the basis of the reduction reaction of GSH and selenium-containing substances, oxidation of selenium is effectively protected by utilizing a protective atmosphere, and it is ensured that the selenium element is combined with GSH in a stable valence state. Finally, a novel selenium nano material (GSH-Se) is synthesized, and the GSH-Se has antioxidant ability and GSH-Px-like activity, can relieve oxidative stress induced primary yak follicular granular cell and mouse ovary steroid hormone synthesis disorder, cell apoptosis and mitochondrial division fusion abnormality, and can be used for preparing a novel selenium nano material (GSH-Se). Meanwhile, the GSH-Se can regulate and control steroid hormone synthesis and cell apoptosis of the yak follicular granular cells by inducing mitochondrial fusion, and a new thought is provided for application of the GSH-Se to improvement of female reproductive performance.

The invention belongs to the technical field of preparation of steroid hormone drugs, and particularly relates to a preparation method of an alpha epoxy steroid compound, which comprises the following steps: by taking a steroid compound I containing a carbon-carbon double bond as a raw material, adding an alkaline reagent, a catalyst and hydrogen peroxide in a solvent clarified state, carrying out epoxy synthesis reaction to obtain an alpha epoxy steroid compound II; wherein the catalyst refers to 3 ', 5'-dichloro-2, 2, 2-trifluoroacetophenone, and the catalyst refers to 3 ', 5'-dichloro-2, 2, 2-trifluoroacetophenone. The invention effectively solves the problems of many isomerization byproducts, low yield, long production period and high comprehensive production cost in the prior art, and provides the preparation method of the alpha epoxy steroid compound with high selectivity and obvious economic benefit, so that the process is more suitable for industrial mass production.

The present application is applicable to the field of medical devices, and discloses a liquid chromatography-mass spectrometry apparatus and a control method for the liquid chromatography-mass spectrometry apparatus. The liquid chromatography-mass spectrometry apparatus comprises a sample storage device, a pretreatment device, a chromatography device, a mass spectrometry detection device, and a controller. The chromatography device comprises a test solution preparation channel, a test solution transfer assembly, a liquid phase fluid delivery assembly, and a chromatography column. An operating pressure outputted by the liquid phase fluid delivery assembly is less than or equal to 20 MPa. The length of the chromatography column is greater than or equal to 3 mm and less than or equal to 35 mm. A sample comprises a target substance to be measured, and the target substance to be measured comprises at least one of vitamin D, a steroid hormone, and a catecholamine. The present application reduces costs of the liquid chromatography-mass spectrometry apparatus.

The invention provides a steroid hormone double-bond selective hydrogenation catalyst as well as a preparation process and application thereof, and belongs to the technical field of catalysts. The preparation process of the hydrogenation catalyst comprises the following steps: (1) preparing a carrier loaded with an active metal: dispersing the carrier in a cerium-containing metal salt ethanol solution, stirring, and carrying out suction filtration, drying and temperature programming roasting after stirring to prepare the carrier loaded with the active metal; (2) loading a bimetal carrier: dipping the active metal-loaded carrier in the precursor solution, carrying out ultrasonic-assisted dipping, and then dropwise adding an alkali solution to adjust the pH value of the solution so as to obtain a bimetal-loaded carrier solution; and (3) reduction: slowly dropwise adding a reducing agent solution into the solution, and after reduction is completed, separating, washing and carrying out vacuum drying to obtain the catalyst. The preparation process is green and safe, and the prepared catalyst can be used for selective hydrogenation reaction of steroid hormone double bonds and has relatively high selectivity and conversion rate.

The invention discloses a method for improving yield of progesterone in yarrowia lipolytica, which comprises the following steps: (1) constructing a biosensor responding to steroid hormone, and the nucleotide sequence of the sensor is as shown in SEQ ID No. 52; (2) a biosensor responding to steroid hormones is connected with a lipid synthesis key gene DGA1 in yarrowia lipolytica and spliced with a linearized pYRSB carrier, a recombinant plasmid pDTY316 is obtained, and the nucleotide sequence of the gene DGA1 is shown as SEQ ID No.57; the nucleotide sequence of the plasmid pYRSB is as shown in SEQ ID No. 54; (3) transforming the recombinant plasmid pDTY316 into a recombinant strain yDTY314 to obtain a recombinant strain yDTY316; and (4) carrying out shake flask fermentation on the recombinant strain yDTY316 to realize high yield of progesterone. Experiments prove that the yield of the progesterone obtained by the method is high.

The invention discloses a preparation method of a steroid methyl ketone compound, belongs to the technical field of preparation of steroid hormone drugs, and particularly relates to a preparation method for preparing a methyl ketone compound shown as a formula II from a steroid corticoid intermediate chloro compound shown as a formula I through zinc powder dechlorination reduction. The solvent is alcohol, and performing dechlorination reduction to obtain the compound shown in the formula II. The method disclosed by the invention is thorough in reduction, wide in substrate applicability and good in safety, can greatly improve the purity and yield of the product in the step, does not need to use a large amount of acid as a solvent, is beneficial to synthesis of downstream corticoid and safe index control operation, and is particularly suitable for a corticoid synthesis process and industrial production thereof.

The invention provides an electron beam irradiation solid waste treatment method, and particularly provides a steroid hormone mushroom dreg treatment method which comprises the following steps: 1) performing first mixing treatment on methanol and to-be-treated steroid hormone mushroom dregs to obtain a first mixed solution; 2) adding calcium chloride into the first mixed solution to obtain a second mixed solution; and 3) carrying out ionizing radiation treatment on the second mixed solution so as to remove steroid hormone substances in the steroid hormone mushroom dregs. According to the method, steroid androgen pollutants in the mushroom dregs can be efficiently removed.

The invention relates to the technical field of detection, in particular to a solid-phase extraction-liquid chromatography-mass spectrometry method for simultaneously detecting 43 steroid hormones in a water body or sludge, which is characterized by comprising enrichment purification, pretreatment and solid-phase extraction and detection, and ultra-high performance liquid chromatography-tandem mass spectrometry. The method specifically comprises the following steps: 1, collecting a sample; 2, treating a water body sample; step 3, sludge sample treatment; step 4, solid-phase extraction; 5, preparing a target object standard curve solution; 6, preparing an internal standard substance mixed standard solution; 7, preparing a sample solution added with an internal standard substance and a mixed standard curve solution; step 8, liquid chromatography-mass spectrometry on-machine detection; and 9, quantifying by an internal standard method. The method is suitable for simultaneous enrichment, purification and detection of 43 steroid hormones of multiple environmental media.

The present invention relates to a method for detecting steroid hormones in serum, comprising the following steps: (1) adding 0.1 to 20 mg of a magnetic solid phase extractant to each milliliter of a sample to be tested, mixing, magnetically separating, and removing liquid; adding a washing solution, mixing, magnetically separating, and removing liquid; adding an eluent for elution, mixing, magnetically separating, and collecting the eluent to obtain a test solution; (2) detecting the hormone content in the test solution by liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate, and 17α-hydroxyprogesterone or a combination thereof; the magnetic solid phase extractant comprises a magnetic core ferrosoferric oxide, a middle layer structure silica wrapping the magnetic core, and an outer layer mesoporous structure, wherein the outer layer mesoporous structure is selected from any one of polystyrene and polystyrene divinylbenzene or a combination thereof. The present invention has the characteristics of good stability, high accuracy and sensitivity, strong specificity, high recovery rate, simple and controllable operation, fast analysis speed, etc., and can be used for simultaneous quantitative detection of multiple hormones in clinical practice.

The invention relates to a preparation method and application of a releasing agent, and belongs to the technical field of steroid hormone releasing agents. The releasing agent is prepared from the following components in percentage by mass: 0.2 to 1 percent of an amide compound, 0.02 to 0.06 percent of a metal chelate, 0.05 to 0.15 percent of a preservative, 0.2 to 0.5 percent of a surfactant, 0.015 to 0.045 percent of a steroid heterocyclic compound and the balance of deionized water, wherein the amide compound is a composition of sodium alpha-mercaptoethanesulfonate and N-methylacetamide, and the amide compound is a compound of sodium alpha-mercaptoethanesulfonate and N-methylacetamide; the steroid heterocyclic compound is a composition of methyltestosterone, danazol and stanolone. According to the universal releasing agent, the four hormones in a sample can be effectively released from respective binding proteins to be in a free state, and clinical test data of the sample can be obtained by measuring the content of the free hormones, so that the measured test result is more accurate.

The invention provides a plate and frame filtering device and application in steroid hormone medicine production. The plate and frame filtering device comprises a rack, and the rack comprises two parallel supporting beams; lap joint parts are arranged on the two sides of the plate frame body; supporting rails are horizontally arranged on the two outer sides of the rack, and a driving mechanism is arranged on the rack. A driving arm and a first elastic piece are vertically hinged to the lifting piece, a lifting arm is horizontally and movably arranged on the side portion of the lifting piece, and the free end of the lifting arm is vertically and rotationally sleeved with a lifting wheel; the fixing frame is horizontally arranged on the outer side of the rack, a plurality of fixing seats are arranged on the fixing frame at intervals, the X sides of the fixing seats are inclined lifting slopes, the-X sides of the fixing seats are vertical, and an obtuse angle is formed between each lifting slope and the top of the fixing frame; the telescopic mechanism is used for controlling the lifting arm to enter and exit the lifting part. According to the plate and frame filtering device and the application of the plate and frame filtering device in steroid hormone medicine production, the using effect is better.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

The present invention discloses a method for simultaneously detecting a plurality of fat-soluble vitamins and steroid hormones, comprising the following steps: adding an internal standard to a serum sample, mixing, and then adding methyl tert-butyl ether for extraction; extracting the components to be tested into an organic solvent, centrifuging, drying the extract with nitrogen, re-dissolving, and then injecting and analyzing the extract on a liquid chromatography-mass spectrometry system. The detection method of the present invention can simultaneously detect the contents of five fat-soluble vitamins and seventeen steroid hormones in serum (plasma), and simultaneously test a total of 22 substances to be tested, which is accurate and can be quantitatively detected. The present invention adopts a scheme that changes the conventional prior art of using the isotope of the substance to be tested as the internal standard substance, and adopts trenbolone cyclohexylmethyl carbonate as the internal standard, which can simultaneously quantify 22 substances to be tested, greatly reducing the workload.

This invention provides a soft material, container, liquid calibrator, and test kit sealed with a sealing agent. The sealing agent in the soft material is a steroid compound. This invention solves the problem in existing technologies where steroid hormones are easily adsorbed by the soft material of the container during storage, and is applicable to the field of soft materials.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The application discloses a preparation method of estradiol-17beta-alkyl acid ester and belongs to the technical field of preparation and processing of steroid hormone drugs. The method uses estradiol as raw material, dissolves the raw material in an organic solvent, adds alkyl acid, and then adds a catalyst and a dehydrating agent to generate a mixture of 3, 17beta-dialkyl acid ester and 17beta-alkyl acid ester, and then adds a hydrolysis reagent to generate 17beta-alkyl acid ester. The method uses a new dehydrating agent and a specific catalyst to solve the problems of high temperature, low yield, expensive and toxic reagents in the estradiol 17beta-hydroxyl esterification process of the traditional process, and the process conditions are more moderate, the purity and yield of the prepared product are higher, the reagents are more green and environmentally friendly, and the method is more in line with the industrial development trend of green chemistry.

The invention discloses a rapid detection method for 27 steroid hormones in mangrove plants. The method comprises the following steps: S1, putting a mangrove plant sample subjected to freeze drying, grinding and sieving into a centrifugal tube A, and then adding an isotope internal standard for balancing; adding acetonitrile, carrying out vortex, ultrasonic extraction and centrifugation, and taking supernate; s2, methanol and acetonitrile are sequentially added, vortex, ultrasonic extraction and centrifugation are performed, and supernate obtained after acetonitrile extraction in the S1, supernate obtained after methanol extraction in the S2 and supernate obtained after acetonitrile extraction in the S2 are combined; s3, transferring the supernate into a centrifugal tube B containing filler, carrying out vortex centrifugation, and putting the supernate into a glass test tube; s4, extracting the filler in S3 again by using acetonitrile, carrying out vortex centrifugation, and combining the two supernatants in a glass test tube; s5, carrying out nitrogen blowing concentration on the solution in the glass test tube, and fixing the volume in methanol; s6, determining the steroid hormones and the metabolites thereof by using high performance liquid chromatography-tandem mass spectrometry, and obtaining the content level of the steroid hormones and the metabolites thereof in the plants through data analysis and quantitative calculation.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention relates to a preparation method of steroid hormone blank matrix serum. The invention particularly relates to a preparation method of steroid hormone blank matrix serum. The preparation method comprises the following steps: activating HLB (Hydrophile Lipophile Balance) magnetic beads by using an ammonium acetate methanol solution; the method comprises the following steps: mixing serum with activated HLB magnetic beads, and extracting steroid hormones in the serum; and applying a magnetic field to adsorb the HLB magnetic beads, and collecting a first supernatant. The invention also relates to a method for extracting the steroid hormone in the serum and a method for regenerating the HLB magnetic beads for extracting the steroid hormone. The method is simple, rapid and safe to operate; the requirements of automatic equipment can be met, and the method is particularly suitable for automatic large-scale preparation; meanwhile, the prepared blank matrix serum is suitable for being used as a blank control or a blank diluent in detection.