64 results about "Steroid hormone" patented technology

The invention discloses a steroid hormone degrading bacterium as well as a screening method and application thereof, and relates to the field of biological medicines, the steroid hormone degrading bacterium comprises klebsiella pneumoniae (KP2022 Klebsiella pneumoniae) with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2025478. The steroid hormone degrading bacterium provided by the invention has the capability of efficiently degrading dexamethasone, is beneficial to degrading dexamethasone in the environment, is conveniently obtained by adopting a screening method, and provides a sample for research on degradation of steroid hormones.

The invention discloses an antioxidant nano material as well as a preparation method and application thereof, and belongs to the technical field of material preparation. On the basis of the reduction reaction of GSH and selenium-containing substances, oxidation of selenium is effectively protected by utilizing a protective atmosphere, and it is ensured that the selenium element is combined with GSH in a stable valence state. Finally, a novel selenium nano material (GSH-Se) is synthesized, and the GSH-Se has antioxidant ability and GSH-Px-like activity, can relieve oxidative stress induced primary yak follicular granular cell and mouse ovary steroid hormone synthesis disorder, cell apoptosis and mitochondrial division fusion abnormality, and can be used for preparing a novel selenium nano material (GSH-Se). Meanwhile, the GSH-Se can regulate and control steroid hormone synthesis and cell apoptosis of the yak follicular granular cells by inducing mitochondrial fusion, and a new thought is provided for application of the GSH-Se to improvement of female reproductive performance.

The present application is applicable to the field of medical devices, and discloses a liquid chromatography-mass spectrometry apparatus and a control method for the liquid chromatography-mass spectrometry apparatus. The liquid chromatography-mass spectrometry apparatus comprises a sample storage device, a pretreatment device, a chromatography device, a mass spectrometry detection device, and a controller. The chromatography device comprises a test solution preparation channel, a test solution transfer assembly, a liquid phase fluid delivery assembly, and a chromatography column. An operating pressure outputted by the liquid phase fluid delivery assembly is less than or equal to 20 MPa. The length of the chromatography column is greater than or equal to 3 mm and less than or equal to 35 mm. A sample comprises a target substance to be measured, and the target substance to be measured comprises at least one of vitamin D, a steroid hormone, and a catecholamine. The present application reduces costs of the liquid chromatography-mass spectrometry apparatus.

The invention provides a steroid hormone double-bond selective hydrogenation catalyst as well as a preparation process and application thereof, and belongs to the technical field of catalysts. The preparation process of the hydrogenation catalyst comprises the following steps: (1) preparing a carrier loaded with an active metal: dispersing the carrier in a cerium-containing metal salt ethanol solution, stirring, and carrying out suction filtration, drying and temperature programming roasting after stirring to prepare the carrier loaded with the active metal; (2) loading a bimetal carrier: dipping the active metal-loaded carrier in the precursor solution, carrying out ultrasonic-assisted dipping, and then dropwise adding an alkali solution to adjust the pH value of the solution so as to obtain a bimetal-loaded carrier solution; and (3) reduction: slowly dropwise adding a reducing agent solution into the solution, and after reduction is completed, separating, washing and carrying out vacuum drying to obtain the catalyst. The preparation process is green and safe, and the prepared catalyst can be used for selective hydrogenation reaction of steroid hormone double bonds and has relatively high selectivity and conversion rate.

The invention discloses a method for improving yield of progesterone in yarrowia lipolytica, which comprises the following steps: (1) constructing a biosensor responding to steroid hormone, and the nucleotide sequence of the sensor is as shown in SEQ ID No. 52; (2) a biosensor responding to steroid hormones is connected with a lipid synthesis key gene DGA1 in yarrowia lipolytica and spliced with a linearized pYRSB carrier, a recombinant plasmid pDTY316 is obtained, and the nucleotide sequence of the gene DGA1 is shown as SEQ ID No.57; the nucleotide sequence of the plasmid pYRSB is as shown in SEQ ID No. 54; (3) transforming the recombinant plasmid pDTY316 into a recombinant strain yDTY314 to obtain a recombinant strain yDTY316; and (4) carrying out shake flask fermentation on the recombinant strain yDTY316 to realize high yield of progesterone. Experiments prove that the yield of the progesterone obtained by the method is high.

The invention relates to the technical field of detection, in particular to a solid-phase extraction-liquid chromatography-mass spectrometry method for simultaneously detecting 43 steroid hormones in a water body or sludge, which is characterized by comprising enrichment purification, pretreatment and solid-phase extraction and detection, and ultra-high performance liquid chromatography-tandem mass spectrometry. The method specifically comprises the following steps: 1, collecting a sample; 2, treating a water body sample; step 3, sludge sample treatment; step 4, solid-phase extraction; 5, preparing a target object standard curve solution; 6, preparing an internal standard substance mixed standard solution; 7, preparing a sample solution added with an internal standard substance and a mixed standard curve solution; step 8, liquid chromatography-mass spectrometry on-machine detection; and 9, quantifying by an internal standard method. The method is suitable for simultaneous enrichment, purification and detection of 43 steroid hormones of multiple environmental media.

The present invention relates to a method for detecting steroid hormones in serum, comprising the following steps: (1) adding 0.1 to 20 mg of a magnetic solid phase extractant to each milliliter of a sample to be tested, mixing, magnetically separating, and removing liquid; adding a washing solution, mixing, magnetically separating, and removing liquid; adding an eluent for elution, mixing, magnetically separating, and collecting the eluent to obtain a test solution; (2) detecting the hormone content in the test solution by liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate, and 17α-hydroxyprogesterone or a combination thereof; the magnetic solid phase extractant comprises a magnetic core ferrosoferric oxide, a middle layer structure silica wrapping the magnetic core, and an outer layer mesoporous structure, wherein the outer layer mesoporous structure is selected from any one of polystyrene and polystyrene divinylbenzene or a combination thereof. The present invention has the characteristics of good stability, high accuracy and sensitivity, strong specificity, high recovery rate, simple and controllable operation, fast analysis speed, etc., and can be used for simultaneous quantitative detection of multiple hormones in clinical practice.

The invention provides a plate and frame filtering device and application in steroid hormone medicine production. The plate and frame filtering device comprises a rack, and the rack comprises two parallel supporting beams; lap joint parts are arranged on the two sides of the plate frame body; supporting rails are horizontally arranged on the two outer sides of the rack, and a driving mechanism is arranged on the rack. A driving arm and a first elastic piece are vertically hinged to the lifting piece, a lifting arm is horizontally and movably arranged on the side portion of the lifting piece, and the free end of the lifting arm is vertically and rotationally sleeved with a lifting wheel; the fixing frame is horizontally arranged on the outer side of the rack, a plurality of fixing seats are arranged on the fixing frame at intervals, the X sides of the fixing seats are inclined lifting slopes, the-X sides of the fixing seats are vertical, and an obtuse angle is formed between each lifting slope and the top of the fixing frame; the telescopic mechanism is used for controlling the lifting arm to enter and exit the lifting part. According to the plate and frame filtering device and the application of the plate and frame filtering device in steroid hormone medicine production, the using effect is better.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

This invention provides a soft material, container, liquid calibrator, and test kit sealed with a sealing agent. The sealing agent in the soft material is a steroid compound. This invention solves the problem in existing technologies where steroid hormones are easily adsorbed by the soft material of the container during storage, and is applicable to the field of soft materials.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The application discloses a preparation method of estradiol-17beta-alkyl acid ester and belongs to the technical field of preparation and processing of steroid hormone drugs. The method uses estradiol as raw material, dissolves the raw material in an organic solvent, adds alkyl acid, and then adds a catalyst and a dehydrating agent to generate a mixture of 3, 17beta-dialkyl acid ester and 17beta-alkyl acid ester, and then adds a hydrolysis reagent to generate 17beta-alkyl acid ester. The method uses a new dehydrating agent and a specific catalyst to solve the problems of high temperature, low yield, expensive and toxic reagents in the estradiol 17beta-hydroxyl esterification process of the traditional process, and the process conditions are more moderate, the purity and yield of the prepared product are higher, the reagents are more green and environmentally friendly, and the method is more in line with the industrial development trend of green chemistry.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention relates to a preparation method of steroid hormone blank matrix serum. The invention particularly relates to a preparation method of steroid hormone blank matrix serum. The preparation method comprises the following steps: activating HLB (Hydrophile Lipophile Balance) magnetic beads by using an ammonium acetate methanol solution; the method comprises the following steps: mixing serum with activated HLB magnetic beads, and extracting steroid hormones in the serum; and applying a magnetic field to adsorb the HLB magnetic beads, and collecting a first supernatant. The invention also relates to a method for extracting the steroid hormone in the serum and a method for regenerating the HLB magnetic beads for extracting the steroid hormone. The method is simple, rapid and safe to operate; the requirements of automatic equipment can be met, and the method is particularly suitable for automatic large-scale preparation; meanwhile, the prepared blank matrix serum is suitable for being used as a blank control or a blank diluent in detection.

The invention discloses application of cinnamyl aldehyde, and cinnamyl aldehyde can regulate synthesis and secretion of androgen testosterone and can be applied to related products for regulating synthesis and secretion of androgen testosterone. In addition, tests prove that cinnamyl aldehyde increases synthesis and secretion of testicular interstitial cell androgen by improving activity of a steroid hormone synthesis nuclear receptor promoter and up-regulating expression of steroid hormone synthetase.

The invention relates to the technical field of analysis and detection, in particular to a detection method for quantitative urine steroid hormone based on efficient enzymolysis. The invention establishes a detection method for accurately quantifying 35 steroid hormones in urine by combining a high performance beta glucuronide enzymatic dissociation method with high performance liquid chromatography-tandem mass spectrometry. According to the method, the pretreatment time required by clinical determination of urine steroid hormones can be remarkably shortened, single-needle quantitative detection of 35 steroid hormones is realized, and the method has a wide application prospect.

The invention provides a microbial enzyme catalytic synthesis method of 20 alpha-hydroxyprogesterone, and belongs to the technical field of biosynthesis. The invention establishes an efficient, green and high-selectivity 20 alpha-hydroxyprogesterone biosynthesis method, and the method has the direct effects that the directional hydroxylation of the C20 site of the substrate progesterone is realized by utilizing a recombinase technology, the generation of byproducts is effectively avoided, the subsequent separation and purification process is simplified, the reaction conditions are mild, the cost is low, and the method is suitable for industrial production. The energy consumption and the safety risk are reduced; from the aspect of product application significance, the method provides a stable and reliable material source for large-scale preparation of 20 alpha-hydroxyprogesterone, the environmental protection property and economical efficiency of the production process are remarkably improved, a solid material foundation is laid for in-depth study on the biological activity and application of the compound, and the method is suitable for industrial production. And a beneficial technical reference is provided for green biological manufacturing of other steroid hormone medicines.

The invention provides a mass spectrum detection system and a mass spectrum detection method for on-site rapid quantification of steroid hormones. The detection system comprises: a pretreatment module for pretreating a biological sample to obtain a first sample; the derivatization module is used for carrying out derivatization treatment on the first sample to obtain a second sample; and the capillary electrophoresis-mass spectrometry analysis module is used for sequentially carrying out electrophoretic separation and mass spectrometry detection on the second sample and carrying out qualitative and quantitative analysis on the steroid hormone in the second sample. Therefore, by adopting the detection system disclosed by the invention, the ionization efficiency and the analysis sensitivity of the steroid hormone can be improved. The capillary electrophoresis-mass spectrometry module is adopted, so that the whole analysis process is efficient and rapid, the analysis time is effectively controlled within 10 minutes, and the requirement of instant field detection can be fully met due to the high efficiency.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

This invention discloses sterol side-chain cleaving enzymes and 3β-hydroxysteroid dehydrogenases / Δ from different sources. 4,5 Amino acid sequences, coding sequences, and applications of isomerases. This invention, based on synthetic biology and molecular pharmacognosy, explores sterol side-chain cleaving enzymes and 3β-hydroxysteroid dehydrogenases / Δ from different plant sources. 4,5 Isomerases, which will yield sterol side-chain cleavage enzymes and 3β-hydroxysteroid dehydrogenases / Δ 4,5 The coding sequences of the isomerases were constructed into expression cassettes, and using Saccharomyces cerevisiae as the chassis strain, highly efficient pregnenolone or progesterone synthesizing strains were obtained. The pregnenolone yield of the constructed strains reached as high as 14.46±2.16 mg / L, and the progesterone yield reached as high as 32.78±1.84 mg / L. This achieved high-level synthesis of pregnenolone and progesterone by genetic engineering methods, providing an important foundation for the green synthesis of steroid hormones.

The invention relates to the technical field of biomedical analysis, in particular to a clinical steroid hormone determination method which comprises the following steps: repeatedly treating human plasma by adopting a solid-phase extraction method, using octadecylsilane bonded silica gel filler, extracting to remove endogenous steroid hormone, and obtaining a human plasma blank matrix; the method comprises the following steps: respectively preparing a steroid hormone stock solution and a loratadine internal standard solution, taking the human plasma blank matrix treated in the step 1, adding the loratadine internal standard solution, treating with a 2% formic acid solution, extracting by using an HLB solid-phase extraction column, centrifugally concentrating an eluent, redissolving for LC-MS / MS analysis, carrying out sample analysis by adopting an LC-MS / MS method, and determining the content of the loratadine in the human plasma blank matrix. And detecting in an ESI positive ionization mode. Therefore, multiple steroid hormones can be detected at the same time, the coverage concentration range is wide, endogenous interference can be effectively removed, the detection accuracy is improved, the sensitivity is high, the repeatability is good, and the method is suitable for high-throughput analysis of clinical samples.

The invention provides a kit for simultaneously detecting six steroid hormones and application of the kit. The steroid hormones comprise testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone. The kit comprises extract liquor, a mobile phase additive and a standard quality control product. The kit provided by the invention can be used for simultaneously detecting testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone, and has the advantages of high accuracy, low quantitation limit and wide detection range.

The invention discloses a method for enhancing cholesterol uptake of saccharomyces cerevisiae, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out transcription inhibition factors MOT3 and ROX1 of genes related to cholesterol uptake and transport of saccharomyces cerevisiae; the transcription activating factors SUT1 and UPC2-1 of the cholesterol transporter gene are over-expressed; expression of sterol esterifying enzyme ARE2 is enhanced, so that uptake and storage of exogenous cholesterol by the saccharomyces cerevisiae are further enhanced. According to the method disclosed by the invention, the uptake and storage of cholesterol by the saccharomyces cerevisiae are enhanced, and an important reference is provided for construction of a steroid hormone synthesis chassis taking cholesterol as a substrate.

The invention discloses application of silibinin in preparation of a medicine for treating autoimmune hepatitis, and belongs to the field of biological medicine application. The silybin has a good liver protection effect, and a pathological experiment combined with a related mouse disease model in development of autoimmune liver injury pathogenesis research finds that the silybin can well relieve inflammation caused by the autoimmune liver injury, so that the silybin can replace or assist a traditional steroid hormone therapy, and the silybin has a good application prospect. And side effects caused by traditional therapy are reduced.

The invention relates to the technical field of dairy product detection, in particular to a method for simultaneously detecting three steroid hormone substances in raw milk. The three steroid hormone substances are estradiol, ethinyl estradiol and estrone respectively, and the detection method comprises the following steps: 1) removing protein in raw milk to obtain a sample solution to be detected; (2) carrying out qualitative and quantitative analysis on the three steroid hormone substances in the sample solution to be detected by adopting a high performance liquid chromatography-ultraviolet detection method; wherein the qualitative analysis is based on the retention time of each substance, and the quantitative analysis is based on a standard curvilinear equation to calculate the concentration. According to the detection method, the dependence on expensive instruments and professional operators is remarkably reduced while high sensitivity and accuracy are guaranteed, the cost is low, and the method is particularly suitable for rapid screening of grassroots units and large-batch samples.