41 results about "Steroid hormone" patented technology

The present application is applicable to the field of medical devices, and discloses a liquid chromatography-mass spectrometry apparatus and a control method for the liquid chromatography-mass spectrometry apparatus. The liquid chromatography-mass spectrometry apparatus comprises a sample storage device, a pretreatment device, a chromatography device, a mass spectrometry detection device, and a controller. The chromatography device comprises a test solution preparation channel, a test solution transfer assembly, a liquid phase fluid delivery assembly, and a chromatography column. An operating pressure outputted by the liquid phase fluid delivery assembly is less than or equal to 20 MPa. The length of the chromatography column is greater than or equal to 3 mm and less than or equal to 35 mm. A sample comprises a target substance to be measured, and the target substance to be measured comprises at least one of vitamin D, a steroid hormone, and a catecholamine. The present application reduces costs of the liquid chromatography-mass spectrometry apparatus.

The invention provides a steroid hormone double-bond selective hydrogenation catalyst as well as a preparation process and application thereof, and belongs to the technical field of catalysts. The preparation process of the hydrogenation catalyst comprises the following steps: (1) preparing a carrier loaded with an active metal: dispersing the carrier in a cerium-containing metal salt ethanol solution, stirring, and carrying out suction filtration, drying and temperature programming roasting after stirring to prepare the carrier loaded with the active metal; (2) loading a bimetal carrier: dipping the active metal-loaded carrier in the precursor solution, carrying out ultrasonic-assisted dipping, and then dropwise adding an alkali solution to adjust the pH value of the solution so as to obtain a bimetal-loaded carrier solution; and (3) reduction: slowly dropwise adding a reducing agent solution into the solution, and after reduction is completed, separating, washing and carrying out vacuum drying to obtain the catalyst. The preparation process is green and safe, and the prepared catalyst can be used for selective hydrogenation reaction of steroid hormone double bonds and has relatively high selectivity and conversion rate.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

This invention provides a soft material, container, liquid calibrator, and test kit sealed with a sealing agent. The sealing agent in the soft material is a steroid compound. This invention solves the problem in existing technologies where steroid hormones are easily adsorbed by the soft material of the container during storage, and is applicable to the field of soft materials.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention provides a microbial enzyme catalytic synthesis method of 20 alpha-hydroxyprogesterone, and belongs to the technical field of biosynthesis. The invention establishes an efficient, green and high-selectivity 20 alpha-hydroxyprogesterone biosynthesis method, and the method has the direct effects that the directional hydroxylation of the C20 site of the substrate progesterone is realized by utilizing a recombinase technology, the generation of byproducts is effectively avoided, the subsequent separation and purification process is simplified, the reaction conditions are mild, the cost is low, and the method is suitable for industrial production. The energy consumption and the safety risk are reduced; from the aspect of product application significance, the method provides a stable and reliable material source for large-scale preparation of 20 alpha-hydroxyprogesterone, the environmental protection property and economical efficiency of the production process are remarkably improved, a solid material foundation is laid for in-depth study on the biological activity and application of the compound, and the method is suitable for industrial production. And a beneficial technical reference is provided for green biological manufacturing of other steroid hormone medicines.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

This invention discloses sterol side-chain cleaving enzymes and 3β-hydroxysteroid dehydrogenases / Δ from different sources. 4,5 Amino acid sequences, coding sequences, and applications of isomerases. This invention, based on synthetic biology and molecular pharmacognosy, explores sterol side-chain cleaving enzymes and 3β-hydroxysteroid dehydrogenases / Δ from different plant sources. 4,5 Isomerases, which will yield sterol side-chain cleavage enzymes and 3β-hydroxysteroid dehydrogenases / Δ 4,5 The coding sequences of the isomerases were constructed into expression cassettes, and using Saccharomyces cerevisiae as the chassis strain, highly efficient pregnenolone or progesterone synthesizing strains were obtained. The pregnenolone yield of the constructed strains reached as high as 14.46±2.16 mg / L, and the progesterone yield reached as high as 32.78±1.84 mg / L. This achieved high-level synthesis of pregnenolone and progesterone by genetic engineering methods, providing an important foundation for the green synthesis of steroid hormones.

The invention relates to the technical field of biomedical analysis, in particular to a clinical steroid hormone determination method which comprises the following steps: repeatedly treating human plasma by adopting a solid-phase extraction method, using octadecylsilane bonded silica gel filler, extracting to remove endogenous steroid hormone, and obtaining a human plasma blank matrix; the method comprises the following steps: respectively preparing a steroid hormone stock solution and a loratadine internal standard solution, taking the human plasma blank matrix treated in the step 1, adding the loratadine internal standard solution, treating with a 2% formic acid solution, extracting by using an HLB solid-phase extraction column, centrifugally concentrating an eluent, redissolving for LC-MS / MS analysis, carrying out sample analysis by adopting an LC-MS / MS method, and determining the content of the loratadine in the human plasma blank matrix. And detecting in an ESI positive ionization mode. Therefore, multiple steroid hormones can be detected at the same time, the coverage concentration range is wide, endogenous interference can be effectively removed, the detection accuracy is improved, the sensitivity is high, the repeatability is good, and the method is suitable for high-throughput analysis of clinical samples.

The invention discloses a method for enhancing cholesterol uptake of saccharomyces cerevisiae, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out transcription inhibition factors MOT3 and ROX1 of genes related to cholesterol uptake and transport of saccharomyces cerevisiae; the transcription activating factors SUT1 and UPC2-1 of the cholesterol transporter gene are over-expressed; expression of sterol esterifying enzyme ARE2 is enhanced, so that uptake and storage of exogenous cholesterol by the saccharomyces cerevisiae are further enhanced. According to the method disclosed by the invention, the uptake and storage of cholesterol by the saccharomyces cerevisiae are enhanced, and an important reference is provided for construction of a steroid hormone synthesis chassis taking cholesterol as a substrate.

The invention relates to the technical field of dairy product detection, in particular to a method for simultaneously detecting three steroid hormone substances in raw milk. The three steroid hormone substances are estradiol, ethinyl estradiol and estrone respectively, and the detection method comprises the following steps: 1) removing protein in raw milk to obtain a sample solution to be detected; (2) carrying out qualitative and quantitative analysis on the three steroid hormone substances in the sample solution to be detected by adopting a high performance liquid chromatography-ultraviolet detection method; wherein the qualitative analysis is based on the retention time of each substance, and the quantitative analysis is based on a standard curvilinear equation to calculate the concentration. According to the detection method, the dependence on expensive instruments and professional operators is remarkably reduced while high sensitivity and accuracy are guaranteed, the cost is low, and the method is particularly suitable for rapid screening of grassroots units and large-batch samples.

The present invention provides novel compounds of formula (I) as CYP11A1 inhibitors, useful for the treatment of symptoms and diseases affected by steroid hormone receptors, such as symptoms and diseases dependent on androgen receptor (AR), including prostate cancer. The invention also discloses a preparation method, a pharmaceutical composition and application thereof.

The invention discloses a rodent sterilant synergist and application of the rodent sterilant synergist in preparation of sterilant bait. According to the invention, ketoconazole with relatively strong CYP enzyme inhibitory activity is taken as a synergist and is compounded with mifepristone and baits to form the compound sterilant for harmful rats. Wherein ketoconazole can effectively inhibit the activity of CYP enzyme, significantly reduce the metabolic clearance rate of mifepristone, improve the oral bioavailability of mifepristone, maintain the same sterility prevention and control effect, greatly reduce the use dosage of the sterilant, and realize reduction and synergy. The compound pest sterilant provided by the invention solves the problems of poor killing effect, short lasting effect, fast rebound, high cost, easy generation of drug resistance and accidental killing of natural enemies and non-target organisms in the existing single use of a steroid hormone sterilant, and provides a new solution for sustainable control of pest.

The invention discloses a formula and application of a compound infertility agent containing ketoconazole and quinestrol and based on CYP enzyme activity inhibition. Ketoconazole, quinestrol and specific baits are compounded to form the reduction and synergism type compound rodent pest sterilant, ketoconazole can effectively inhibit CYP enzyme activity, obviously reduce the metabolic clearance rate of quinestrol and improve the oral bioavailability of quinestrol, and the use dosage of the sterilant is greatly reduced while the same sterility prevention and control effect is maintained. The compound pest sterilant provided by the invention solves the common problems of poor killing effect, short lasting period, fast rebound, high cost, easy generation of drug resistance, accidental killing of natural enemies and non-target organisms and the like existing in the existing single use of steroid hormone sterilant, and provides a new solution way for sustainable control of pest.

This invention discloses the application of a ketone reductase mutant in the catalytic reduction reaction of steroid ketone compounds, belonging to the field of biocatalysis and enzyme engineering technology. The ketone reductase mutant is based on the amino acid sequence of the wild-type ketone reductase ChKRED20, and the following mutations are made: mutant ChKRED20-M4C3: S153L, Q97L, Y188G, and H145A; mutant ChKRED20-M4C5: S153L, Q97L, Y188A, and H145A. This invention provides mutants ChKRED20-M4C3 and ChKRED20-M4C5 that successfully achieve the efficient synthesis of steroid hormones, represented by testosterone. Through carrier-free immobilization technology, efficient recycling and reuse of the ketone reductase mutant are achieved. Based on this, a microfluidic bed is designed to realize the continuous flow production of testosterone.

Compounds of Formula (I), pharmaceutical compositions or kits thereof are disclosed. Wherein A1 is C (O) or CHRz1, Rz1 is OH, amino, imino, halogen, (C1-C5) alkoxy, OC (O) (C1-C5) alkyl, and OSO2-amino; a2 is CH2 or O; r1 is hydrogen, a (C1-C5) alkyl group optionally substituted by one or more Z substituents, a (C1-C5) alkoxy group optionally substituted by one or more Z substituents, a (C2-C5) alkenyl group optionally substituted by one or more Z substituents, a (C2-C5) alkynyl group optionally substituted by one or more Z substituents; (C3-C8) cycloalkyl, aryl or heteroaryl groups; z is halogen, OH, (C1 to C5) alkyl, (C1 to C5) haloalkyl, (C1 to C5) alkoxy, (C1 to C5) haloalkoxy, or amino; r2 is a heterocyclic aromatic ring having 5, 6 or 7 members and having at least one N, S and / or O; r3 is a (C1-C5) alkyl group, which is substituted by one or more Br, Br76; i, I123, I124 or I131 substitutions; m is an integer selected from 0 to 2; n is an integer selected from 0 to 2; and p is an integer selected from 0 to 5. The compounds of formula I are inhibitors of 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) for use in the treatment or prevention of steroid hormone dependent diseases such as breast cancer, lung cancer, prostate cancer, ovarian cancer, uterine cancer, endometrial cancer and endometrial hyperplasia. (I).

The invention relates to an application of an IDH1 gene in porcine ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the porcine ovarian granular cells and / or steroid hormone synthesis and / or reproductive performance related gene expression. According to the invention, porcine ovarian granular cells are taken as objects, and the expression of the IDH1 gene is regulated to verify that the IDH1 gene is taken as a key target spot for regulating and controlling the functions of the porcine ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. A new molecular target is provided for improving the reproductive performance of the sow, more reliable technical support is provided for regulating and controlling the ovarian function, and a new thought and technical guidance are provided for mechanism research of reproductive diseases and preparation and development of related drugs.

The application provides a kit for simultaneously detecting 90 kinds of steroid hormones and metabolites thereof in a urine sample, a detection method and a sample pretreatment method, the kit comprising 90 kinds of steroid hormones and metabolites thereof mixed standard working solution, internal standard working solution, matrix, redissolving agent and eluent, and further comprising liquid-liquid extraction agent, stabilizer, acid solution, buffer and enzymatic hydrolysis solution. 2 The application is characterized in that the R of the standard curve is greater than 0.990. The application realizes the simultaneous detection of 90 kinds of steroid hormones and metabolites thereof in a urine sample, and has the advantages of simple and rapid operation, high throughput, real-time, accurate and effective monitoring of the level of steroid hormones and metabolites thereof in human urine, and wide application prospect.

This invention discloses a novel use of posaconazole to enhance the immune response in the immunotherapy of breast cancer. Specifically, this invention provides a drug combination comprising posaconazole and a PD-L1 inhibitor. The effects of this invention are: (1) using posaconazole to prepare a drug for treating breast cancer, especially triple-negative breast cancer (TNBC), achieving "repurposing of an old drug"; (2) clarifying its core mechanism of action, clarifying that the anti-tumor effect of clinically commonly used doses of posaconazole depends on the host immune system, rather than traditional direct cytotoxicity, specifically by inhibiting CYP11A1 and other related enzymes in the tumor, thereby reducing immunosuppressive steroid hormones, providing a new drug and target for tumor immunotherapy; (3) providing a technical solution for the combined treatment of posaconazole and PD-L1 inhibitors (such as anti-PD-L1 antibodies), further enhancing anti-tumor immunity and improving the treatment effect of TNBC.

The present invention belongs to the technical field of preparation of steroid hormone drugs, and specifically relates to a preparation method for α epoxy steroid compounds. The method comprises: using a steroid compound I containing a carbon-carbon double bond as a starting material, and adding a basic reagent, a catalyst and hydrogen peroxide into a solvent under the state of complete dissolution of the starting material in the solvent, and carrying out an epoxide synthesis reaction to obtain an α epoxy steroid compound II, wherein the catalyst refers to 3',5'-dichloro-2,2,2-trifluoroacetophenone. The present invention effectively solves the problems in the prior art such as excessive isomeric by-products, low yield, long production period and high overall production costs, and provides the preparation method for the α epoxy steroid compounds with high selectivity and significant economic benefits, so that the process is more suitable for the requirement of industrial mass production.

Provided are an estimation device, an estimation method, and a program capable of estimating the steroid hormone concentration in the future from the point of time when data is measured. The estimation device estimates, by using an estimation model that receives physiological data at a certain point of time as an input and outputs an estimation value of the steroid hormone concentration after a prescribed time, a first steroid hormone concentration after a prescribed time by using first physiological data at a certain point of time. The estimation model is a model trained by using second physiological data for training at a certain point of time and a measurement value of a second steroid hormone concentration for training after a prescribed time.

The invention relates to an accelerant for up-regulating key genes of a steroid hormone synthesis pathway of scapharca broughtonii and application of the accelerant, and belongs to the technical field of aquaculture, the accelerant is prepared from the following components in parts by mass: 15-20 parts of cholesterol, 8-10 parts of linolenic acid, 10-12 parts of phosphatidylcholine, 0.5-0.8 part of zinc sulfate, 30-40 parts of L-arginine and 0.5-0.75 * 10 <-3 > part of vitamin D3. Core genes (SF-1, StAR, 17 beta-HSD14 and CYP17) of a steroid hormone synthesis pathway of scapharca broughtonii are specifically focused, synergistic up-regulation of the genes is realized through a gonad maturation accelerator, a core molecular pathway of gonad development is directly activated, and the regulation effect is accurate and stable.

The invention relates to a preparation method of mesenchymal stem cells for promoting hair follicle regeneration. The preparation method comprises the following steps: performing adherent culture on primary mesenchymal stem cells, and performing three-stage induction culture to obtain the mesenchymal stem cells with the effect of promoting hair follicle regeneration, a culture medium used in each stage of induction culture contains a chemical small molecule composition, and the chemical small molecule composition comprises at least one of a steroid hormone, a GSK-3beta inhibitor, a DNA methyltransferase inhibitor, a BMP receptor inhibitor, a histone deacetylase inhibitor and a Smoothed receptor agonist. According to the preparation method, through three-stage step-by-step induction culture, hair follicles in MSCs are activated by chemical small molecules to generate a key signal channel, so that the MSCs are developed into the MSCs with hair follicle regeneration promotion performance, and the survival rate, the directional differentiation efficiency and the hair induction capability of the transplanted MSCs in a hair follicle microenvironment are remarkably improved.

The present application relates to the field of animal breeding, and particularly to a method for gender reversal of crustacean animals. The present application provides a method for gender reversal of crustacean animals, taking crustacean animals after metamorphosis, feeding with steroid hormones, and breeding. The experiment proves that sex hormones of vertebrates play a role in gender differentiation of crustacean animals, and when the concentration of MT added in feed is 2000 mg / kg, PL2 and PL25 male juvenile prawns can be induced; when the concentration of E2 added in feed is 2000 mg / kg, PL25 juvenile prawns can be induced to be female, and male juvenile prawns of PL30 can be reversed to female prawns. The experiment determines the best gender reversal period and hormone concentration in different development stages of juvenile prawns, and observes the egg-carrying "pseudo-female prawns", which lays a foundation for determining the chromosome pairing of juvenile prawns and monosex breeding.