36results about How to "Excellent chromatographic performance" patented technology

The present invention is a surface-modified base matrix comprised of a porous polymeric base matrix onto which branched hydrophilic polyhydroxy-functional polymers have been covalently attached, wherein the polyhydroxy-functional polymers are hyperbranched polymers presenting a degree of branching (DB) of at least about 0.2 and each polymer is tethered to the base matrix at two or more points. The present matrix can for example be a cross-linked carbohydrate material, such as agarose, and the hyperbranched hydrophilic polymer can e.g. be a copolymer of epichlorohydrin and a sugar. The invention also relates to a method of surface-modification of a porous base matrix by activating functional hydroxy groups thereon and contacting the activated matrix with a hydrophilic hyperbranched hydroxy-functional polymer.

A method and system for detecting residual poison in human body are provided. Using the disclosed HPLC-Chip-mass spectrometry (MS)/MS and/or HPLC-MS/MS method to detect the residual poison, the method of the present invention mainly includes sample preparation, liquid chromatography and mass spectrometry. The method of the present invention has advantages of low sample size, high specificity, low detection limit, high sensitivity, low cost, high accuracy and stability, etc.

The invention discloses an amino group-containing ionic liquid-modified graphene oxide-wrapped silica gel. A preparation method for the silica gel comprises the following steps: (1) adding silica gelcontaining silicon hydroxyl groups on its surface into the solvent toluene, then adding aminopropyl triethoxysilane, and carrying out heating reflux for surface amination of the silica gel so as to obtain aminopropyl silica gel; (2) adding the aminopropyl silica gel into an aqueous solution of graphene oxide and carrying out a reaction so as to obtain graphene oxide-modified silica gel; (3) preparing amino group-containing ionic liquid by reacting 2-bromoethylamine hydrobromide and 1-methylimidazole in the solvent acetonitrile; and (4) reacting the graphene oxide-modified silica gel with the amino group-containing ionic liquid in the solvent methanol and successively carrying out centrifugation, washing with deionized water and drying. The graphene-oxide-wrapped silica gel modified by theamino-group-containing ionic liquid can be used as a chromatographic stationary-phase material for separation and analysis of substances and has excellent chromatographic performance.

A method for detecting a compound danshen tablet by adopting an HPLC-UV-ELSD process is disclosed. Characteristic spectrums of danshen root and radix notoginseng in the compound danshen tablet are studied by adopting gradient elution and detection wavelength changing in liquid chromatography and an ultraviolet detector-tandem evaporative light-scattering detector. The method can perform total-information control on the compound danshen tablet, simplifies a detection process, saves analysis time, and can be used for quality control and comprehensive evaluation of the compound danshen tablet.

The invention relates to a chirality ligand exchange spectrum fixing phase of organic-inorganic core frame, and relative preparation, which are used to analyze the chirallity compound, especially for detaching the DL-amino acid and DL-alcoholic acid antimer, wherein on the surface of inorganic particle, grafting polymer macromolecule monomer on the surface, to synthesize the particle with core and frame structure; then using the chirality selection factor chemical decoration, to prepare the fixing phase. The inventive method is simple, with high antimer selectivity, quick detaching speed, and long service life. The accompanying diagram is the detaching spectrum of DL-His (histidine) on fixing phase (CS-LEP I).

The invention relates to a quality control method of a medicine preparation, and especially relates to thin layer chromatogram authentication and content determination of smilax glabra in a xiaojiean preparation. The method is a thin layer chromatogram authentication and / or content determination method formulated by using a special component in the smilax glabra of the xiaojiean preparation as an index component, wherein the special component is astilbin, and the xiaojiean preparation is a traditional Chinese medicine compound preparation prepared from 1100 parts of leatherleaf mahonia, 750 parts of thin evodia, 750 parts of motherwort, 750 parts of spatholobus stem, 900 parts of smilax glabra and 750 parts of fructus forsythiae. The thin layer chromatogram authentication method with a strong specialization can authenticate smilax glabra accurately and reliably, and a generally employed HPLC can determine the astilbin content in the xiaojiean preparation, so as to produce valuable significance for monitoring and controlling medicinal material purchase, preparation production process and preparation quality in market, and ensuring product safety, effectiveness and quality stabilization. According to the method, problem of confused smilax glabra basic material provided in the market can be solved effectively to ensure that xiaojiean preparation meets the national medicine standards strictly.

The invention discloses a gold nanoparticle modification based open tubular column, and a preparation method and application thereof, and belongs to the technical field of chromatography. The preparation method comprises the following steps: (1) synthesis of a gold nanoparticle solution by a chemical reduction method; (2) pretreatment on the capillary; (3) silanization of the capillary; and (4) modification on inner wall of capillary. The preparation method provided by the invention employs 3-mercapto propyl-trimethoxy silane as a silanization reagent, so that the gold nanoparticles and mercapto groups are modified on the inner surface of the quartz column by chemical bonding; then lauryl mercaptan is added, and the mercapto group in the lauryl mercaptan can react with gold nanoparticles as well, thereby bonding on the inner wall of the capillary. The invention uses gold nanoparticles easily characteristics and SH groups covalently bonded, gold nanoparticles were prepared by modified open tubular capillary column. The invention utilizes the characteristic of gold nanoparticles of easy covalent bonding with -SH groups to prepare the gold nanoparticle modified open tubular column. The technology of using gold nanoparticles as a stationary phase of the open tubular capillary column improves the disadvantages of low volume and small phase ratio of the open tubular capillary column.

The invention relates to a preparation method and an application of an omega-diamine derivatization beta-cyclodextrin bonded SBA-15 chiral stationary phase. The preparation method comprises steps as follows: 6-p-tosylation beta-cyclodextrin and omega-diamine are taken as raw materials, the omega-diamine is a solvent, and 6-(omega-diamine)-beta-cyclodextrin ligand is prepared through heating; the 6-(omega-diamine)-beta-cyclodextrin ligand and 3-isocyanato propyl siloxane react to prepare siloxane containing the 6-(omega-diamine)-beta-cyclodextrin ligand; anhydrous N,N-dimethyl formamide is taken as a solvent and heated under the protection of nitrogen, so that the siloxane containing the 6-(omega-diamine)-beta-cyclodextrin ligand reacts with a silanol hydroxyl group on the surface of SBA-15 silica gel, and a coarse product is obtained; and redundant ligand and impurities in holes are cleared, and a final product is obtained. The preparation method is simple, convenient, lower in cost, wide in application and suitable for rapidly preparing the chiral stationary phase.

The invention discloses a preparation method of an oxidized-graphene-modified silica gel chromatography filling material, and belongs to the field of silica gel chromatography filling materials. The preparation method comprises the following steps: adsorbing oxidized graphene to the surface of activated silica gel, and then carrying out a heat treatment under the protection of nitrogen gas so as to fixedly adhere the oxidized graphene to the silica gel surface. The content of the oxidized graphene on the surface of the silica gel chromatography filling material can be controlled by controlling the layers of oxidized graphene which is fixedly adhered to the silica gel surface. In the provided preparation method, the content of carbon on the surface of the silica gel chromatography filling material can be adjusted by controlling the content of oxidized graphene on the surface of the silica gel chromatography filling material so as to meet the application requirements of liquid chromatography, and finally an oxidized-graphene-modified silica gel chromatography filling material with an excellent chromatography performance is obtained.

The invention relates to a padding used to do molecule brand solid-phase extraction and it's preparing method. The carrier of the padding is glass bead which has been surface modified. The preparing method of padding is using liquid film method to form a polymeric film layer with molecule brand at the surface of carrier.

The present disclosure relates to the determination of pesticides, e.g., polar pesticides, in a sample using chromatography. The present disclosure can provide direct analysis of polar pesticides, including anionic polar pesticides, using high performance liquid chromatography. The polar pesticides are sufficiently retained and resolved to allow for multiple polar pesticide determinations in a single analysis.

The invention discloses a preparation method of a high-purity porous spherical silica gel filler, comprising the following preparation steps: purification of raw material tetraethoxysilane, synthesisand post-treatment of polyethoxysilane, synthesis of the high-purity spherical silica filler, and washing aging, roasting and drying. The invention provides the preparation method of the high-purity porous spherical silica gel filler. The spherical silica gel prepared by the preparation method has good regularity, and the metal impurity content in the porous high-purity silica gel is determined byusing an ICP full spectrum direct reading plasma emission spectrometer, and is as follows: Al: 3.5 mg/kg, Ca: 1 mg/kg, Fe: 1.5 mg/kg, Mg: 2.0 mg/kg, and Na: 1 mg/kg. The metal impurity content is lower than that of domestic silica gel, and the chromatographic performance is good. Under normal-phase and reversed-phase conditions, the column efficiency is about 65,000, and the peak shape is symmetrical. The high-purity porous spherical silica gel filler can be widely used as a chromatographic filler matrix for rapid separation of chemical or biological macromolecules.

The invention discloses a process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin. The process comprises the following steps: extracting and filtering tea leaves, loading the filtered tea extract solution into a chromatographic column filled with spherical polyamide chromatographic resin for chromatography, eluting caffeine and a pigment by using a citric acid aqueous solution as an eluent, then eluting tea polyphenol by using ultrapure water and finally eluting tea polyphenol by using an ethanol aqueous solution as a mobile phase, and finally, concentrating and crystallizing the collected tea polyphenol eluent to obtain a colorless and transparent high-quality tea polyphenol crystal finished product. According to the process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin, the extraction method is simple, eco-friendly, free of pollution and low in production cost, the requirements that the purity of the tea polyphenol is larger than 99% and the extraction rate is larger than 90% can be met only through one-step chromatographic purification, the prepared tea polyphenol is high in yield, high inextraction rate and high in quality, the requirements of more fields can be met, and therefore wider market requirements are met.

The invention relates to a manufacture method for a molecule branding filling material that forms molecule branding polymer film layer on the surface of carrier. It could sharply decrease the consumption of raw material. The polymer could distribute more equally, compact and full on the surface of carrier. The method is easy to operate and is suitable to take large scale producing.

The invention discloses a preparation method of a spherical silica gel filler. The preparation method comprises the following preparation steps: purification of a raw material tetraethoxysilane, synthesis and post-treatment of polyethoxysilane, and synthesis and drying of the spherical silica gel filler. According to the preparation method of the spherical silica gel filler provided by the invention, the spherical silica gel prepared by the preparation method is good in regularity and chromatographic performance, and can be widely applied as a chromatographic filler matrix for rapidly separating chemical or biological macromolecules.

The invention discloses a sol-gel chitosan grafting cyclodextrin capillary electrochromatography open tubular column as well as a preparation method and application thereof, which belongs to the field of a chromatographic technology. The preparation method of the sol-gel chitosan grafting cyclodextrin capillary electrochromatography open tubular column comprises the following steps: (1) capillary pretreatment; (2) capillary surface silanization; (3) sol-gel preparation; and (4) inner capillary wall decoration. A stationary phase of the capillary open tubular column is chitosan grafting cyclodextrin sol-gel. The sol-gel chitosan grafting cyclodextrin capillary electrochromatography open tubular column provided by the invention combines the identification property of cyclodextrin and the adsorption property of chitosan. An obtained coating has the characteristics of firmness in bonding, stable property and good repeatability, and can be used within a wider pH (Potential of Hydrogen) range; the direction and the size of electroosmotic flow of the coating can be controlled by adjusting a pH value of a mobile phase; and the obtained coating has a good separation effect on Xanthopterin isomers and phenoxy carboxylic acid herbicides.