58results about How to "Rapid separation test" patented technology

The invention provides a miniflow control chip which has a simple structure and can continuously analyzing single-cell contents at high speed and an operation method. The invention is characterized in that a sheath flow passageway is arranged at each side of a sample injection passageway of a cross-miniflow control chip; through adjusting static pressure difference between liquid storage tanks, cell suspension and sheath flow liquid simultaneously flow out of a sample reservoir and a sheath flow liquid reservoir; under the action of sheath flow, single cells in the cell suspension rank in a row to enter into a separation passageway from the sample injection passageway in turn, contact with a film dissolving agent in the process of movement and conduct rapid film dissolution at the inlet of the separation passageway; the film-dissolved cell contents fully enter into the separation passageway to be continuously separated at high speed under the action of an field stress generated at two ends of the separation passageway; and laser induces fluorescence detection. As the solution entering into the separation passageway is a mixed solution of the sheath flow liquid and normal saline in the cell suspension, the invention can also greatly reduce the concentration of the normal saline entering into the separation passageway and markedly lower band broadening caused by Joule heat when electrophoresis is carried out.

A blood perfusion separation detecting and imaging method for a bone surface capillary comprises the following steps: programming transmitting and receiving beams meeting the bone surface multimode leakage Lamb wave condition, constructing bone surface multimode leakage Lamb wave microbubble mother wavelets, then achieving mode separation detection of incidence, reflection and multi-mode leakage Lamb wave aliasing RF (Radio Frequency) echo signals by adopting the bone surface multimode leakage Lamb wave microbubble wavelet method, and finally achieving radiography imaging and blood perfusion parameter imaging of the transformation capillary for the bone surface multimode leakage Lamb wave microbubble wavelets. The blood perfusion separation detecting and imaging method can effectively improve the resolution and the radiography tissue ratio and suppress aliasing interference of the bone surface multimode leakage Lamb waves to radiography imaging of the bone surface tissue capillary, thereby achieving great significance on early diagnosis and evaluation of malignant bone tumors.

The invention belongs to the field of analytical chemistry and particularly relates to a method of separating and testing (S)-Rivaroxaban and enantiomers thereof by liquid chromatography. The method for separating and testing (S)-Rivaroxaban and enantiomers (impurities) thereof by liquid chromatography is characterized in that a polysaccharide derivative is used as a chiral chromatographic column of filler and a mixed solution of low alcohol or low alkane or low alcohol is used as a moving phase. According to the separation and detection method provided by the invention, (S)-Rivaroxaban can be effectively separated from enantiomers thereof, wherein the separation degree reaches over 1.5 and complete baseline separation is achieved, so that the quality of (S)-Rivaroxaban can be accurately and effectively controlled. The separation method provided by the invention can be used for separating and detecting the (S)-Rivaroxaban and enantiomers thereof within 80 minutes. The method provided by the invention can be used for simply, quickly and accurately separating and detecting (S)-Rivaroxaban and optical isomers thereof.

The invention discloses a basic orange II molecular imprinting solid-phase extraction filler, which is molecular imprinting polymer micro-particles which are prepared by taking basic orange II as template molecules, acrylamide as functional monomer, maleated rosin ethylene glycol acrylate as a cross-linking agent and azo-bis-iso-butyrynitrile as an initiator. The solid-phase extraction filler has a memory function to the stereo structure of basic orange II molecules, can realize the specificity, selective separation and enrichment of the basic orange II, is packed into the solid extraction column, can be used for detecting the selective separation, purification and enrichment of the basic orange II in sample extraction solution containing the basic orange II in foods, realizes high-sensitivity and high-selectivity detection for food samples containing the basic orange II, has stable recovery rate and small relative standard deviation and has the characteristics of simpler, quicker and more efficient operation and the like.

The invention relates to a method for using a liquid chromatography to separate and measure apremilast and enantiomer thereof. The method includes that using spherical silica gel coated with chiral polymer at surface as a chiral chromatographic column, using alkane-different concentrations of polarity organic solvent as mobile phase, wherein the polarity organic solvent is composed of first organic solvent and second organic solvent, the alkane is selected from normal hexane, normal heptane, cyclohexane and methylene dichloride, the first organic solvent is isopropanol, and the second organic solvent is selected from methanol, ethanol and acetonitrile. The method for using the liquid chromatography to separate and measure the apremilast and enantiomer thereof solves the problem that the apremilast and enantiomer thereof are difficult to separate for the separation and measurement, and accordingly the controllable quality of the apremilast and the preparation thereof is guaranteed.

The invention discloses a solid-phase catecholamine extraction functional composite material with magnetic 3D graphene nanoparticles as a core. A functional polymer, which is arranged on the surface of the solid-phase catecholamine extraction functional composite, is mainly synthesized from ethyl orthosilicate, trimethoxysilylpropanethiol and atropic acid, has a specific affinity site for catecholamines and is of a crosslinked network-like structure. Accordingly, the invention also provides a corresponding preparation method. The solid-phase extraction functional composite material is used foradsorption and dynamic extraction and separation of catecholamines such as trace adrenaline, which are illegally added in the pretreatment of cosmetic samples with complex matrix, and can be appliedto rapid separation and detection of catecholamines. The preparation method is simple, the cost is low, the recovery rate is high, the relative standard deviation is small, and the method has the characteristics of less use of organic solvents and convenient, fast and efficient solid phase extraction operation.

The invention discloses a serum sugar spectrum parting method based on a micro-fluidic chip, and relates to the field of sugar analysis. The method comprises the steps of selecting a buffer system, selecting an electrophoresis chip, a serum sample, carrying out labeling and electrophoresis, and the like. By combination of a chip electrophoresis technology and a laser-induced fluorescence detection system, 12 N-sugar chains appearing in serum are separated within 8 minutes, so that the shortcoming that a conventional bio-chemical blood analysis method cannot be used to analyze an N-sugar spectrum is overcome. With high resolution and high separation and analysis capacity for the N-sugar spectrum of the serum, chip electrophoresis is expected to be one of analysis measures for quickly, conveniently and efficiently separating and detecting the N-sugar spectrum of the clinical serum sample.

This invention discloses one method to isolate small and intense fat protein of micro flow chip electrophoresis, which comprises sample pre-process micro flow chip, electrophoresis device.

The invention discloses a core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material, and a preparation method and application thereof. According to the molecularly imprinted solid phase extraction magnetic material, surface-aminated Fe3O4 nano-particles serve as a magnetic core, and a rhodamine 6G molecularly imprinted polymer membrane serves as a shell. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has a memory function for a three-dimensional structure of rhodamine 6G molecules, and rhodamine 6G can be specifically and selectively separated and enriched. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material is applied to selective adsorption and dynamic extraction and separation of a trace amount of rhodamine 6G in the food sample analysis pretreatment; the rhodamine 6G can be quickly separated and detected; according to the method, the recovery rate is high and the relative standard deviation is small; the core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has the characteristics of small using amount of organic solvents, simplicity, quickness and high efficiency in operation, and the like.

The invention provides a dry chemical analyzer for detecting a micro-fluidic chip. The dry chemical analyzer comprises a lifting mechanism, a centrifuging mechanism and a chip holding mechanism, the chip holding mechanism is provided with a chip fixing part for locking the micro-fluidic chip and a chip piercing part for jacking a liquid reagent pre-packaging device in the sample cell of the micro-fluidic chip, the chip piercing part is fixed on the chip fixing member, the chip fixing part is connected to the centrifuging mechanism, and the lifting mechanism drives the centrifuging mechanism tomove up and down. The dry chemical analyzer for detecting the micro-fluidic chip of the invention realizes whole-process automation in detection of the micro-fluidic chip provided with the pierce endof the liquid reagent pre-packaging device at the top of the sample cell, realizes automation in entry, separation, location and detection of the chip only through adding a sample to be detected intothe chip, can rapidly separate and detect to obtain all indicators, omits the steps of manually transferring the chip and pressing and piercing the chip, and greatly improves the detection efficiency.

The invention relates to a separation and measurement method of besifloxacin hydrochloride and an isomer of besifloxacin hydrochloride. The method is characterized by adopting a chiral chromatographic column which takes cyclodextrin as a filler and adopting a mobile phase system formed by a buffer salt solution and an organic solvent. After the method is adopted, the besifloxacin and the enantiomer of the besifloxacin are effectively separated from each other, and the separation degree of the besifloxacin and the enantiomer of the besifloxacin reaches up to more than 3.5, so that the quality of besifloxacin hydrochloride can be accurately and effectively controlled. Compared with the existing patents and literatures, the method does not need a complicated derivative process and an expensive derivatization reagent on the whole, and is simple in sample pretreatment process, short in analysis time and high in operability. After the method is used, the besifloxacin and the optical isomer of the besifloxacin are simply, rapidly and accurately separated and detected.

The invention discloses a method for pretreating a fluoroquinolone veterinary medicine antibiotic sample in aquaculture waste water and measuring the content of the fluoroquinolone, and belongs to thetechnical fields of analysis and detection of trace organic matters in water environments. An aquaculture waste water pretreatment device is provided, and comprises a filtering and purifying column and a solid phase extraction column which are connected in series; the content measuring method comprises pretreatment, enrichment, elution and HPLC-MS / MS detection; and the fluoroquinolone veterinarymedicine antibiotic in the aquaculture waste water can be detected. The filtering and purifying column is connected in series with the solid phase extraction column, an interference substance on the measuring result of the fluoroquinolone veterinary medicine antibiotic in a substrate is removed, and the pretreatment process is simplified. The self-filling type filtering and purifying column is used for automatically filling filtering fillers and the column body can be repeatedly used, so that the economic cost is reduced. The method for measuring the content of the fluoroquinolone veterinary medicine antibiotic sample in the aquaculture waste water is established, so that various fluoroquinolone veterinary medicine antibiotics can be separated and detected rapidly within short time, and the advantages of high operability and high efficiency are achieved.

The invention discloses a high-throughput analysis method for simultaneously detecting 33 plastic additives in a food contact material based on liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analysis and detection. In allusion to the technical problems of incapability of simultaneously detecting multiple types of plastic additives at high flux, long detectiontime, low detection efficiency and the like in the existing detection method, the method disclosed by the invention can be used for detecting and analyzing 33 types of plastic additives within 12 minutes, has the advantages of high sensitivity, better separation degree, short detection time, high detection efficiency and the like, can meet the high-throughput detection requirements of various plastic additives in the food contact material, provides theoretical guidance and technical support for monitoring of various types of plastic additives in food safety supervision at the same time, guarantees the health and life safety of people, and has a good application prospect.

The invention provides a method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting a high performance liquid chromatography. In the process of determining L-alanine isopropyl ester hydrochloride by adopting the high performance liquid chromatography, a chromatographic column taking octadecyl bonded silica gel as a filling agent is adopted, the related substances comprise impurities, namely L-alanine methyl ester hydrochloride and L-alanine ethyl ester hydrochloride, and sodium heptanesulfonate is used as an ion pair reagent. According to the invention, the analysis method is short in analysis time, good in peak shape symmetry, high in recovery rate and high in sensitivity; and by adopting the method, the related substances of the L-alanine isopropyl ester hydrochloride can be rapidly separated and detected, and the content of impurities (L-alanine methyl ester and L-alanine ethyl ester) in the L-alanine isopropyl ester hydrochloride can be accurately determined.

The invention discloses Sudan red I molecularly-imprinted solid phase extraction padding. The Sudan red I molecularly-imprinted solid phase extraction padding refers to molecularly-imprinted polymer particles which is prepared in a way that Sudan red I is taken as a template molecule, acrylamide is taken as a function monomer, maleated rosin glycol acrylate is taken as a cross-linking agent, and azoisobutyronitrile is taken as an initiating agent. The solid phase extraction padding has a memory function on a three-dimensional structure of a molecule of the sudan red I, the specificity, the selective separation and the enrichment of the sudan red I are realized, the padding is filled so as to form a solid phase extraction column, and the solid phase extraction column is used for detecting the selective separation, purification and the enrichment of the Sudan red I in an extraction solution containing a sudan red I food sample; and the high sensitivity and high selectivity detection on the food sample containing the sudan red I is realized, and the method provided by the invention has the characteristics of being high in recovery ratio, small in relative standard deviation, simple and rapid to operate, high in efficiency and the like.

The invention discloses a clobetasol propionate molecule capturer. According to a preparation method, a graphene oxide modified polyethylene filter board is taken an internal support body, and synthesis of a clobetasol propionate membrane on the surface of the internal support body is carried out. In synthesis of the molecularly imprinted polymer membrane, clobetasol propionate is taken as a template molecule, 3-aminopropyltriethoxysilane is taken as a functional monomer, ethyl orthosilicate is taken as a cross-linking agent, and glacial acetic acid is taken as a silane hydrolysis condensating agent. The clobetasol propionate molecule capturer is a novel molecular imprinting-solid phase extraction integral material, is adopted in pretreatment of selective adsorption and dynamic extraction separation of trace illegally added clobetasol propionate in cosmetic samples with complex matrix so as to realize rapid separation detection on clobetasol propionate. The invention also discloses the preparation method of the clobetasol propionate molecule capturer. The preparation method is simple; cost is relatively low; organic solvent using amount is low, solid phase extraction operation is simple and rapid, and is high in efficiency.

The invention discloses a method for pretreating enrofloxacin in aquaculture waste water and measuring the content of the enrofloxacin, and belongs to the technical fields of analysis and detection oftrace organic matters in water environments. An aquaculture waste water pretreatment device is provided, and comprises a filtering and purifying column and a solid phase extraction column which are connected in series; the content measuring method comprises pretreatment, enrichment, elution and HPLC-MS / MS detection; and the veterinary medicine antibiotic enrofloxacin in the aquaculture waste water can be detected. The filtering and purifying column is connected in series with the solid phase extraction column, an interference substance on the measuring result of the veterinary medicine antibiotic enrofloxacin in a substrate is removed, and the pretreatment process is simplified. The self-filling type filtering and purifying column is used for automatically filling filtering fillers and the column body can be repeatedly used, so that the economic cost is reduced. The method for measuring the content of the veterinary medicine antibiotic enrofloxacin sample in the aquaculture waste water is established, so that the veterinary medicine antibiotic enrofloxacin can be separated and detected rapidly within short time, and the advantages of high operability and high efficiency are achieved.

The invention discloses a method for determining the content of an enantiomer of L-alanine isopropyl ester hydrochloride by adopting a high performance liquid chromatography, which comprises the following steps: taking a chiral chromatographic column taking amylose bonded silica gel as a filler, taking n-hexane-absolute ethyl alcohol added with 0.1% of diethylamine as a mobile phase, and controlling the volume ratio of the n-hexane to the absolute ethyl alcohol to be (75: 25)-(85: 15); the detection wavelength is 225 + / -5nm; the flow velocity is 1.0 ml / min-1. 2ml / min, and benzoyl chloride is used as a derivatization reagent to prepare a sample. By adopting the method, the L-alanine isopropyl ester hydrochloride isomer can be effectively separated and accurately quantified. The method has the advantages of strong specificity, good precision and high accuracy, and can rapidly and accurately determine the content of the L-alanine isopropyl ester hydrochloride enantiomer.

The invention discloses a PET developing agent precursor TsOP-(+)-DTBZ and a separation and measurement method of an optical isomer thereof and belongs to the field of analytical chemistry. The separation and measurement method is a normal-phase chromatography by means of a Pirkle type chiral stationary phase chromatographic column and with a normal-phase mixed solvent serving as a mobile phase for separation. By means of the method, the PET developing agent precursor and the optical isomer of the PET developing agent precursor can be easily, quickly and accurately separated and detected, and the mass of the precursor TsOP-(+)-DTBZ can be effectively controlled.

The invention discloses a method for simultaneously detecting bisphenol A and 18 phthalate esters based on ultraperformance convergence chromatography. The method adopts ultraperformance convergence chromatography to perform gradient elution. The chromatographic column size is 150 mm x 30 mm, 1.7 [mu]m ACQUITY UPC2 CSHBEH. A mobile phase A is supercritical CO2 and a mobile phase B is an organic modifier. The column temperature is 50 to 70 DEG C. The flow rate is 0.8 to 1.5 ml / min. The system back pressure is 1700 to 2000 psi. The detection wavelength is 200 to 300 nm. The sample injection volume is 1 to 10 [mu]L. The invention can detect bisphenol A and 18 phthalate esters in 5.5 minutes. The invention has high sensitivity, good separation effect, short detection time and high detection efficiency, which can meet daily detection requirements of bisphenol A and phthalate esters plasticizers in food contact materials. The invention also provides theoretical guidance and technical supportfor the simultaneous detection of bisphenol A and 18 phthalate esters, and has a good application prospect.

The invention discloses a method for analyzing the raw material of elagolix sodium and its synthetic intermediate, which is characterized in that the raw material of elagolix sodium and its synthetic intermediate are analyzed by high-performance liquid chromatography. Need testing solution is elagolix sodium, the mixed solution of starting material SM1, SM2, SM3, SM4 and intermediate M1, M2, M3; Select Kromasil-Eternity-5-C18 chromatographic column, with phosphate buffer solution and Acetonitrile was used as the mobile phase, and an ultraviolet detector was selected with a detection wavelength of 200-230nm to analyze the raw material of elagolix sodium and the synthetic intermediate. It solves the problem of separation and detection of elagolix sodium and its synthetic intermediates, and ensures the quality controllability of elagolix sodium raw materials or preparations.

The invention relates to an application system of chemical apparatus analysis, and especially relates to an ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system used for simultaneously detecting folic acid and methotrexate. The invention relates to an ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system. According to the invention, the carbon nanotubes are positively charged through covalent modification, and are absorbed on the surface of bare glass carbon electrode, such that novel carbon nanotube membrane-modified electrode is formed. The modified electrode is adopted as a working electrode, such that methotrexate and folic acid in anion states can be easily absorbed. Compared to bare glass carbon electrode, electrocatalytic oxidation of methotrexate and folic acid are greatly promoted. According to the invention, the modified electrode is applied in combination with ion chromatography in an ion chromatography electrochemical detector, such that methotrexate and folic acid rapid separation and detection are realized. Therefore, a problem that separation cannot be realized by using merely electroanalysis, and defects of low sensitivity, high interference and complicated operation of other detection methods, are overcome. The system is suitable for detections of low-concentration methotrexate and folic acid in complex substrate biological samples.

The invention relates to a rapid separation and detection method of a vinyl triazole product. The method comprises that diazoresin modified porous poly(glyceryl methacrylate) microspheres are used as a filler of a high performance liquid chromatography column; the filler is prepared by the steps of taking uniform-particle-size porous poly(glyceryl methacrylate) microspheres prepared by a multi-step swelling method as a basis, then hydrolyzing the polymer microspheres to obtain hydroxyl, and carrying out optical crosslinking reaction with diazoresin having diazonium groups. Due to the hydrophobicity of the chromatographic column filler, the overall retention time on the vinyl triazole product having hydrophobicity is reduced, and the retention time of a by-product acetic acid is enhanced with use of a secondary amino group of the modified diazoresin, so that the vinyl triazole and the by-product are separated. The method is used for separation and detection of the vinyl triazole product, has the advantages of rapid separation and detection, simple operation and good reproducibility, provides an effective separation means for triazole vinyl, and has relatively high practical value.

The invention discloses a high performance liquid detection method of triton X-100. A reversed-phase C18 chromatographic column and an ELSD detector are adopted, a mobile phase is a mixed solution ofmethanol and water, and isocratic elution is adopted. According to the method, 5-20 [mu]l of a sample is injected, triton X-100 can be effectively detected, the lowest detection concentration is 0.5 [mu]g / ml, the HPLC spectrum baseline is stable, and drifting does not occur. The method can be used for determining the content of triton X-100, has the advantages of high sensitivity, good linear relationship, good reproducibility, high precision and high accuracy, and has important research value in the aspects of industrial analysis, product formula research and the like.

The invention discloses a method for determining antibiotics in human urine through solid phase extraction-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of detection analysis, and the method mainly comprises the following steps: carrying out solid phase extraction treatment on a pretreated human urine sample to obtain a human urine concentrated sample, and carrying out high performance liquid chromatography-tandem mass spectrometry detection. According to the invention, a solid-phase extraction method is adopted to extract and purify a detected sample at the same time, so that the interference of high-concentration inorganic salt, protein, urea and other components in urine can be effectively removed, the matrix interference effect is reduced, and the detection result is more accurate; meanwhile, the method can simultaneously detect the residual conditions of 37 antibiotics in human urine at one time, and has the advantages of high precision, high sensitivity, high stability, high selectivity, low detection limit, more real and reliable detection result and the like.

The present invention relates to an abnormal prothrombin detection kit and an apparatus, and belongs to the technical field of medical devices. The abnormal prothrombin detection kit comprises: a detection strip provided with a plurality of holes having the same size; and a plurality of separately arranged tubes, wherein the tubes comprise a cleaning solution tube, a coated antibody tube, a labeled antibody tube, and a substrate solution tube, each tube has the same upper end portion, and each tube can be removably arranged in each hole of the detection strip. According to the present invention, the positions of the tubes on the detection strip can be arbitrarily changed according to the requirement so as to reduce the detection cost, and the multiple parallel detections can be achieved with only one detection strip.

The invention relates to a method for using PVP / CdS quantum point modification electrode to achieve protein dividing detecting in the field of nanometer modification electrode and biology detecting. The method must be carried out in the HPLC-ECD architecture, which is formed by the protein chromatographic column with protein, the HPLC and working electrode as PVP / CdS quantum point modification electrode. It uses the HPLC technique to divide the protein; the HOLC pump drives the traveling phase and the detected liquid crossed the protein chromatographic column to enter into the ECD directly and uses PVP / CdS quantum point modification electrode to do electrochemist to the divided protein.

The invention discloses a method for simultaneously detecting bisphenol A and 18 phthalate esters based on ultraperformance convergence chromatography. The method adopts ultraperformance convergence chromatography to perform gradient elution. The chromatographic column size is 150 mm x 30 mm, 1.7 [mu]m ACQUITY UPC2 CSHBEH. A mobile phase A is supercritical CO2 and a mobile phase B is an organic modifier. The column temperature is 50 to 70 DEG C. The flow rate is 0.8 to 1.5 ml / min. The system back pressure is 1700 to 2000 psi. The detection wavelength is 200 to 300 nm. The sample injection volume is 1 to 10 [mu]L. The invention can detect bisphenol A and 18 phthalate esters in 5.5 minutes. The invention has high sensitivity, good separation effect, short detection time and high detection efficiency, which can meet daily detection requirements of bisphenol A and phthalate esters plasticizers in food contact materials. The invention also provides theoretical guidance and technical supportfor the simultaneous detection of bisphenol A and 18 phthalate esters, and has a good application prospect.

The invention relates to a method for using PVP / CdS quantum point modification electrode to achieve protein dividing detecting in the field of nanometer modification electrode and biology detecting. The method must be carried out in the HPLC-ECD architecture, which is formed by the protein chromatographic column with protein, the HPLC and working electrode as PVP / CdS quantum point modification electrode. It uses the HPLC technique to divide the protein; the HOLC pump drives the traveling phase and the detected liquid crossed the protein chromatographic column to enter into the ECD directly and uses PVP / CdS quantum point modification electrode to do electrochemist to the divided protein.