58results about How to "Rapid separation test" patented technology

The invention provides a miniflow control chip which has a simple structure and can continuously analyzing single-cell contents at high speed and an operation method. The invention is characterized in that a sheath flow passageway is arranged at each side of a sample injection passageway of a cross-miniflow control chip; through adjusting static pressure difference between liquid storage tanks, cell suspension and sheath flow liquid simultaneously flow out of a sample reservoir and a sheath flow liquid reservoir; under the action of sheath flow, single cells in the cell suspension rank in a row to enter into a separation passageway from the sample injection passageway in turn, contact with a film dissolving agent in the process of movement and conduct rapid film dissolution at the inlet of the separation passageway; the film-dissolved cell contents fully enter into the separation passageway to be continuously separated at high speed under the action of an field stress generated at two ends of the separation passageway; and laser induces fluorescence detection. As the solution entering into the separation passageway is a mixed solution of the sheath flow liquid and normal saline in the cell suspension, the invention can also greatly reduce the concentration of the normal saline entering into the separation passageway and markedly lower band broadening caused by Joule heat when electrophoresis is carried out.

A blood perfusion separation detecting and imaging method for a bone surface capillary comprises the following steps: programming transmitting and receiving beams meeting the bone surface multimode leakage Lamb wave condition, constructing bone surface multimode leakage Lamb wave microbubble mother wavelets, then achieving mode separation detection of incidence, reflection and multi-mode leakage Lamb wave aliasing RF (Radio Frequency) echo signals by adopting the bone surface multimode leakage Lamb wave microbubble wavelet method, and finally achieving radiography imaging and blood perfusion parameter imaging of the transformation capillary for the bone surface multimode leakage Lamb wave microbubble wavelets. The blood perfusion separation detecting and imaging method can effectively improve the resolution and the radiography tissue ratio and suppress aliasing interference of the bone surface multimode leakage Lamb waves to radiography imaging of the bone surface tissue capillary, thereby achieving great significance on early diagnosis and evaluation of malignant bone tumors.

The invention discloses a basic orange II molecular imprinting solid-phase extraction filler, which is molecular imprinting polymer micro-particles which are prepared by taking basic orange II as template molecules, acrylamide as functional monomer, maleated rosin ethylene glycol acrylate as a cross-linking agent and azo-bis-iso-butyrynitrile as an initiator. The solid-phase extraction filler has a memory function to the stereo structure of basic orange II molecules, can realize the specificity, selective separation and enrichment of the basic orange II, is packed into the solid extraction column, can be used for detecting the selective separation, purification and enrichment of the basic orange II in sample extraction solution containing the basic orange II in foods, realizes high-sensitivity and high-selectivity detection for food samples containing the basic orange II, has stable recovery rate and small relative standard deviation and has the characteristics of simpler, quicker and more efficient operation and the like.

The invention discloses a solid-phase catecholamine extraction functional composite material with magnetic 3D graphene nanoparticles as a core. A functional polymer, which is arranged on the surface of the solid-phase catecholamine extraction functional composite, is mainly synthesized from ethyl orthosilicate, trimethoxysilylpropanethiol and atropic acid, has a specific affinity site for catecholamines and is of a crosslinked network-like structure. Accordingly, the invention also provides a corresponding preparation method. The solid-phase extraction functional composite material is used foradsorption and dynamic extraction and separation of catecholamines such as trace adrenaline, which are illegally added in the pretreatment of cosmetic samples with complex matrix, and can be appliedto rapid separation and detection of catecholamines. The preparation method is simple, the cost is low, the recovery rate is high, the relative standard deviation is small, and the method has the characteristics of less use of organic solvents and convenient, fast and efficient solid phase extraction operation.

The invention discloses a serum sugar spectrum parting method based on a micro-fluidic chip, and relates to the field of sugar analysis. The method comprises the steps of selecting a buffer system, selecting an electrophoresis chip, a serum sample, carrying out labeling and electrophoresis, and the like. By combination of a chip electrophoresis technology and a laser-induced fluorescence detection system, 12 N-sugar chains appearing in serum are separated within 8 minutes, so that the shortcoming that a conventional bio-chemical blood analysis method cannot be used to analyze an N-sugar spectrum is overcome. With high resolution and high separation and analysis capacity for the N-sugar spectrum of the serum, chip electrophoresis is expected to be one of analysis measures for quickly, conveniently and efficiently separating and detecting the N-sugar spectrum of the clinical serum sample.

The invention discloses a core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material, and a preparation method and application thereof. According to the molecularly imprinted solid phase extraction magnetic material, surface-aminated Fe3O4 nano-particles serve as a magnetic core, and a rhodamine 6G molecularly imprinted polymer membrane serves as a shell. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has a memory function for a three-dimensional structure of rhodamine 6G molecules, and rhodamine 6G can be specifically and selectively separated and enriched. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material is applied to selective adsorption and dynamic extraction and separation of a trace amount of rhodamine 6G in the food sample analysis pretreatment; the rhodamine 6G can be quickly separated and detected; according to the method, the recovery rate is high and the relative standard deviation is small; the core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has the characteristics of small using amount of organic solvents, simplicity, quickness and high efficiency in operation, and the like.

The invention relates to a separation and measurement method of besifloxacin hydrochloride and an isomer of besifloxacin hydrochloride. The method is characterized by adopting a chiral chromatographic column which takes cyclodextrin as a filler and adopting a mobile phase system formed by a buffer salt solution and an organic solvent. After the method is adopted, the besifloxacin and the enantiomer of the besifloxacin are effectively separated from each other, and the separation degree of the besifloxacin and the enantiomer of the besifloxacin reaches up to more than 3.5, so that the quality of besifloxacin hydrochloride can be accurately and effectively controlled. Compared with the existing patents and literatures, the method does not need a complicated derivative process and an expensive derivatization reagent on the whole, and is simple in sample pretreatment process, short in analysis time and high in operability. After the method is used, the besifloxacin and the optical isomer of the besifloxacin are simply, rapidly and accurately separated and detected.

The invention discloses a method for pretreating a fluoroquinolone veterinary medicine antibiotic sample in aquaculture waste water and measuring the content of the fluoroquinolone, and belongs to thetechnical fields of analysis and detection of trace organic matters in water environments. An aquaculture waste water pretreatment device is provided, and comprises a filtering and purifying column and a solid phase extraction column which are connected in series; the content measuring method comprises pretreatment, enrichment, elution and HPLC-MS/MS detection; and the fluoroquinolone veterinarymedicine antibiotic in the aquaculture waste water can be detected. The filtering and purifying column is connected in series with the solid phase extraction column, an interference substance on the measuring result of the fluoroquinolone veterinary medicine antibiotic in a substrate is removed, and the pretreatment process is simplified. The self-filling type filtering and purifying column is used for automatically filling filtering fillers and the column body can be repeatedly used, so that the economic cost is reduced. The method for measuring the content of the fluoroquinolone veterinary medicine antibiotic sample in the aquaculture waste water is established, so that various fluoroquinolone veterinary medicine antibiotics can be separated and detected rapidly within short time, and the advantages of high operability and high efficiency are achieved.

The invention provides a method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting a high performance liquid chromatography. In the process of determining L-alanine isopropyl ester hydrochloride by adopting the high performance liquid chromatography, a chromatographic column taking octadecyl bonded silica gel as a filling agent is adopted, the related substances comprise impurities, namely L-alanine methyl ester hydrochloride and L-alanine ethyl ester hydrochloride, and sodium heptanesulfonate is used as an ion pair reagent. According to the invention, the analysis method is short in analysis time, good in peak shape symmetry, high in recovery rate and high in sensitivity; and by adopting the method, the related substances of the L-alanine isopropyl ester hydrochloride can be rapidly separated and detected, and the content of impurities (L-alanine methyl ester and L-alanine ethyl ester) in the L-alanine isopropyl ester hydrochloride can be accurately determined.

The invention discloses Sudan red I molecularly-imprinted solid phase extraction padding. The Sudan red I molecularly-imprinted solid phase extraction padding refers to molecularly-imprinted polymer particles which is prepared in a way that Sudan red I is taken as a template molecule, acrylamide is taken as a function monomer, maleated rosin glycol acrylate is taken as a cross-linking agent, and azoisobutyronitrile is taken as an initiating agent. The solid phase extraction padding has a memory function on a three-dimensional structure of a molecule of the sudan red I, the specificity, the selective separation and the enrichment of the sudan red I are realized, the padding is filled so as to form a solid phase extraction column, and the solid phase extraction column is used for detecting the selective separation, purification and the enrichment of the Sudan red I in an extraction solution containing a sudan red I food sample; and the high sensitivity and high selectivity detection on the food sample containing the sudan red I is realized, and the method provided by the invention has the characteristics of being high in recovery ratio, small in relative standard deviation, simple and rapid to operate, high in efficiency and the like.

The invention discloses a clobetasol propionate molecule capturer. According to a preparation method, a graphene oxide modified polyethylene filter board is taken an internal support body, and synthesis of a clobetasol propionate membrane on the surface of the internal support body is carried out. In synthesis of the molecularly imprinted polymer membrane, clobetasol propionate is taken as a template molecule, 3-aminopropyltriethoxysilane is taken as a functional monomer, ethyl orthosilicate is taken as a cross-linking agent, and glacial acetic acid is taken as a silane hydrolysis condensating agent. The clobetasol propionate molecule capturer is a novel molecular imprinting-solid phase extraction integral material, is adopted in pretreatment of selective adsorption and dynamic extraction separation of trace illegally added clobetasol propionate in cosmetic samples with complex matrix so as to realize rapid separation detection on clobetasol propionate. The invention also discloses the preparation method of the clobetasol propionate molecule capturer. The preparation method is simple; cost is relatively low; organic solvent using amount is low, solid phase extraction operation is simple and rapid, and is high in efficiency.

The invention discloses a PET developing agent precursor TsOP-(+)-DTBZ and a separation and measurement method of an optical isomer thereof and belongs to the field of analytical chemistry. The separation and measurement method is a normal-phase chromatography by means of a Pirkle type chiral stationary phase chromatographic column and with a normal-phase mixed solvent serving as a mobile phase for separation. By means of the method, the PET developing agent precursor and the optical isomer of the PET developing agent precursor can be easily, quickly and accurately separated and detected, and the mass of the precursor TsOP-(+)-DTBZ can be effectively controlled.

The invention relates to a rapid separation and detection method of a vinyl triazole product. The method comprises that diazoresin modified porous poly(glyceryl methacrylate) microspheres are used as a filler of a high performance liquid chromatography column; the filler is prepared by the steps of taking uniform-particle-size porous poly(glyceryl methacrylate) microspheres prepared by a multi-step swelling method as a basis, then hydrolyzing the polymer microspheres to obtain hydroxyl, and carrying out optical crosslinking reaction with diazoresin having diazonium groups. Due to the hydrophobicity of the chromatographic column filler, the overall retention time on the vinyl triazole product having hydrophobicity is reduced, and the retention time of a by-product acetic acid is enhanced with use of a secondary amino group of the modified diazoresin, so that the vinyl triazole and the by-product are separated. The method is used for separation and detection of the vinyl triazole product, has the advantages of rapid separation and detection, simple operation and good reproducibility, provides an effective separation means for triazole vinyl, and has relatively high practical value.

The invention discloses a high performance liquid detection method of triton X-100. A reversed-phase C18 chromatographic column and an ELSD detector are adopted, a mobile phase is a mixed solution ofmethanol and water, and isocratic elution is adopted. According to the method, 5-20 [mu]l of a sample is injected, triton X-100 can be effectively detected, the lowest detection concentration is 0.5 [mu]g/ml, the HPLC spectrum baseline is stable, and drifting does not occur. The method can be used for determining the content of triton X-100, has the advantages of high sensitivity, good linear relationship, good reproducibility, high precision and high accuracy, and has important research value in the aspects of industrial analysis, product formula research and the like.

The invention discloses a method for determining antibiotics in human urine through solid phase extraction-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of detection analysis, and the method mainly comprises the following steps: carrying out solid phase extraction treatment on a pretreated human urine sample to obtain a human urine concentrated sample, and carrying out high performance liquid chromatography-tandem mass spectrometry detection. According to the invention, a solid-phase extraction method is adopted to extract and purify a detected sample at the same time, so that the interference of high-concentration inorganic salt, protein, urea and other components in urine can be effectively removed, the matrix interference effect is reduced, and the detection result is more accurate; meanwhile, the method can simultaneously detect the residual conditions of 37 antibiotics in human urine at one time, and has the advantages of high precision, high sensitivity, high stability, high selectivity, low detection limit, more real and reliable detection result and the like.

The present invention relates to an abnormal prothrombin detection kit and an apparatus, and belongs to the technical field of medical devices. The abnormal prothrombin detection kit comprises: a detection strip provided with a plurality of holes having the same size; and a plurality of separately arranged tubes, wherein the tubes comprise a cleaning solution tube, a coated antibody tube, a labeled antibody tube, and a substrate solution tube, each tube has the same upper end portion, and each tube can be removably arranged in each hole of the detection strip. According to the present invention, the positions of the tubes on the detection strip can be arbitrarily changed according to the requirement so as to reduce the detection cost, and the multiple parallel detections can be achieved with only one detection strip.