Ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system

A technology for modifying electrodes and carbon nanotubes, which is applied in the field of application systems for chemical instrument analysis to achieve the effects of good electrical conductivity, promotion of electrocatalytic oxidation, and enhancement of electrochemical signals

Inactive Publication Date: 2012-10-17
ZHEJIANG UNIV
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Problems solved by technology

So far, there is no report on the simultaneous determination of folic acid and methotrexate by ion chromatography-carbon nanotube modified electrode electrochemical detection method

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  • Ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system
  • Ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system
  • Ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system

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Embodiment Construction

[0030] specific implementation plan

[0031] Below in conjunction with accompanying drawing, the present invention will be further specifically described:

[0032] The bare glassy carbon electrode is sequentially made of 0.3 μm, 0.05 μm α-Al 2 o 3 Polished and ultrasonically cleaned in absolute ethanol and deionized water for 5 min successively, Ag / AgCl (saturated KCl solution) was used as the reference electrode, and a platinum wire electrode was used as the counter electrode. First, the MWNTs were treated with concentrated HNO 3 The solution was heated to reflux for 8 hours, and then the resulting black suspension was filtered, washed thoroughly with deionized water, and finally dried under an infrared lamp to obtain carboxylated carbon nanotubes. Accurately weigh 50 mg of carboxylated carbon nanotubes, add them to 5.00 mL of thionyl chloride, heat to reflux at 45 °C for 24 h under nitrogen protection, wash with tetrahydrofuran and dry in vacuo to obtain acid-chlorinated ...

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Abstract

The invention relates to an application system of chemical apparatus analysis, and especially relates to an ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system used for simultaneously detecting folic acid and methotrexate. The invention relates to an ion chromatography-carbon nanotube-modified electrode electrochemical detection analysis system. According to the invention, the carbon nanotubes are positively charged through covalent modification, and are absorbed on the surface of bare glass carbon electrode, such that novel carbon nanotube membrane-modified electrode is formed. The modified electrode is adopted as a working electrode, such that methotrexate and folic acid in anion states can be easily absorbed. Compared to bare glass carbon electrode, electrocatalytic oxidation of methotrexate and folic acid are greatly promoted. According to the invention, the modified electrode is applied in combination with ion chromatography in an ion chromatography electrochemical detector, such that methotrexate and folic acid rapid separation and detection are realized. Therefore, a problem that separation cannot be realized by using merely electroanalysis, and defects of low sensitivity, high interference and complicated operation of other detection methods, are overcome. The system is suitable for detections of low-concentration methotrexate and folic acid in complex substrate biological samples.

Description

technical field [0001] The invention relates to a system for using chemical instrument analysis. In particular, it relates to an ion chromatography-carbon nanotube modified electrode electrochemical detection and analysis system for simultaneous detection of folic acid and methotrexate. [0002] Background technique [0003] Folate (Flolic acid, FA) is chemically called "pteroyl glutamic acid", which belongs to the B group of water-soluble vitamins. It participates in many important reactions in the body and is an important substance for maintaining life activities. It widely exists in green vegetables and is one of the necessary substances for the growth and division of human cells. It is mainly involved in the synthesis of nucleic acids and the synthesis of proteins from amino acids, and also participates in the synthesis of important compounds such as hemoglobin, choline, creatine and adrenaline. . Methotrexate (Methotrexate, MTX) is a homologue of folic acid, which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/26
Inventor 朱作艺习玲玲
Owner ZHEJIANG UNIV
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