Sudan red I molecularly-imprinted solid phase extraction padding, solid phase extraction column as well as preparation method and application of padding

A solid-phase extraction column and molecular imprinting technology, which can be used in the analysis of materials, chemical instruments and methods, and material separation, and can solve the problem of low separation selectivity

Inactive Publication Date: 2013-04-24
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The packing materials of conventional solid phase extraction columns mainly include bonded silica gel solid phase extraction materials, inorganic matrix solid phase extraction materials and organic polymer solid phase extraction materials, and their separation selectivity is not high.

Method used

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  • Sudan red I molecularly-imprinted solid phase extraction padding, solid phase extraction column as well as preparation method and application of padding

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of Sudan Red Ⅰ Molecularly Imprinted SPE Cartridge

[0028] (1) Preparation of Molecularly Imprinted Polymer Microparticles Containing Sudan I

[0029] Weigh 0.2mmol of Sudan Red I and 4mmol of acrylamide, dissolve them in 3mL of ethyl acetate, place them in the dark for 2h, add 2mmol of maleic rosin ethylene glycol acrylate, 0.3g of azobisisobutyronitrile, 3mL of soybean oil, and 10mL of acetic acid Ethyl ester, ultrasonically dissolved to form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.15%; take 145 mL of solution B and place it in a three-necked flask, and install a stirrer and a water separator (note Filled with distilled water), condenser and thermometer, transfer solution A into solution B under stirring, stir at a constant speed (250r / min) at room temperature for 20min; heat to make the reaction system warm up slowly to 75-78°C, and react for 1h; Discard the solutio...

Embodiment 2

[0040] 1. Preparation of Sudan Red Ⅰ Molecularly Imprinted SPE Cartridge

[0041] (1) Preparation of Molecularly Imprinted Polymer Microparticles Containing Sudan I

[0042] Weigh 0.4mmol Sudan Red Ⅰ, 8mmol acrylamide, dissolve in 6mL ethyl acetate, place in the dark for 2h, add 4mmol maleic rosin glycol acrylate, 0.6g azobisisobutyronitrile, 6mL soybean oil, 20mL acetic acid Ethyl ester, ultrasonically dissolved to form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.15%; take 300 mL of solution B and place it in a three-necked flask, and install a stirrer and a water separator (note Filled with distilled water), condenser and thermometer, transfer solution A into solution B under stirring, stir at a constant speed (250r / min) at room temperature for 20min; heat to make the reaction system warm up slowly to 75-78°C, and react for 1h; Discard the solution in the water separator, slowly raise the temp...

Embodiment 3

[0053] 1. Preparation of Sudan Red Ⅰ Molecularly Imprinted SPE Cartridge

[0054] (1) Preparation of Molecularly Imprinted Polymer Microparticles Containing Sudan I

[0055] Weigh 0.6mmol of Sudan Red I and 12mmol of acrylamide, dissolve them in 10mL of ethyl acetate, place them in the dark for 12h, add 6mmol of maleic rosin ethylene glycol acrylate, 0.9g of azobisisobutyronitrile, 10mL of soybean oil, and 30mL of acetic acid Ethyl ester is dissolved by ultrasonic to form a uniform organic phase solution A; solution B is an aqueous solution of sodium lauryl sulfate with a mass concentration of 0.15%; take 450 mL of solution B and place it in a three-necked flask, and install a stirrer and a water separator (note Filled with distilled water), condenser and thermometer, transfer solution A into solution B under stirring, stir at a constant speed (250r / min) at room temperature for 20min; heat to make the reaction system warm up slowly to 75-78°C, and react for 1h; Discard the so...

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Abstract

The invention discloses Sudan red I molecularly-imprinted solid phase extraction padding. The Sudan red I molecularly-imprinted solid phase extraction padding refers to molecularly-imprinted polymer particles which is prepared in a way that Sudan red I is taken as a template molecule, acrylamide is taken as a function monomer, maleated rosin glycol acrylate is taken as a cross-linking agent, and azoisobutyronitrile is taken as an initiating agent. The solid phase extraction padding has a memory function on a three-dimensional structure of a molecule of the sudan red I, the specificity, the selective separation and the enrichment of the sudan red I are realized, the padding is filled so as to form a solid phase extraction column, and the solid phase extraction column is used for detecting the selective separation, purification and the enrichment of the Sudan red I in an extraction solution containing a sudan red I food sample; and the high sensitivity and high selectivity detection on the food sample containing the sudan red I is realized, and the method provided by the invention has the characteristics of being high in recovery ratio, small in relative standard deviation, simple and rapid to operate, high in efficiency and the like.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a Sudan red I molecularly imprinted solid-phase extraction filler, a solid-phase extraction column and a preparation method and application thereof. Background technique [0002] Sudan red is a lipophilic azo compound, mainly including Ⅰ, Ⅱ, Ⅲ and Ⅳ four common types. It is a kind of synthetic azo industrial dyes, mainly used for the coloring of oil paint, floor wax, shoe polish, engine oil, gasoline and other chemical products. . Studies have shown that Sudan red produces corresponding amines and naphthols under the action of reductases in humans and animals, among which aniline has a strong carcinogenic effect on the human body, can induce liver cell gene mutation, and increase human cancer Naphthol also has carcinogenic, teratogenic, sensitizing, and mutagenic potential toxicity, and has a strong stimulating effect on eyes, skin, and mucous membranes. Absorption...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/281B01J20/30G01N30/02G01N30/60C08F220/56C08F2/44C08J9/28
Inventor 李小燕黄春梅雷福厚李梅苏晓濛刘敏李鹏飞
Owner GUANGXI UNIV FOR NATITIES
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