Device for pretreating fluoroquinolone veterinary medicine antibiotic sample in aquaculture waste water as well as content measuring method

Abstract

The invention discloses a method for pretreating a fluoroquinolone veterinary medicine antibiotic sample in aquaculture waste water and measuring the content of the fluoroquinolone, and belongs to thetechnical fields of analysis and detection of trace organic matters in water environments. An aquaculture waste water pretreatment device is provided, and comprises a filtering and purifying column and a solid phase extraction column which are connected in series; the content measuring method comprises pretreatment, enrichment, elution and HPLC-MS / MS detection; and the fluoroquinolone veterinarymedicine antibiotic in the aquaculture waste water can be detected. The filtering and purifying column is connected in series with the solid phase extraction column, an interference substance on the measuring result of the fluoroquinolone veterinary medicine antibiotic in a substrate is removed, and the pretreatment process is simplified. The self-filling type filtering and purifying column is used for automatically filling filtering fillers and the column body can be repeatedly used, so that the economic cost is reduced. The method for measuring the content of the fluoroquinolone veterinary medicine antibiotic sample in the aquaculture waste water is established, so that various fluoroquinolone veterinary medicine antibiotics can be separated and detected rapidly within short time, and the advantages of high operability and high efficiency are achieved.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of trace organic matter in water environment, in particular to a method for sample pretreatment and content determination of fluoroquinolone veterinary drug antibiotics in aquaculture wastewater. Background technique [0002] At present, veterinary antibiotics are widely used worldwide to treat animal diseases and promote healthy growth of animals. With the development of intensive farming in the livestock and poultry industry, veterinary antibiotics have been abused in large quantities. Animals have limited absorption of antibiotics, and about 75% of antibiotics are excreted in the feces and urine in the form of the original drug or metabolites. Many research results have shown that the sewage discharged from farms contains a large amount of veterinary drug antibiotic residues, and aquaculture wastewater is an important source of antibiotics in environmental water bodies. [0003...

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Problems solved by technology

[0005] Chinese Patent Publication No. CN106370748A discloses the determination method and sample pretreatment method of fluoroquinolones in organic fertilizers. However, there is no relevant report on the determination method of fluoroquinolone veterinary drug antibiotics in aquaculture wastewater

[0006] Compared with other environmental water bodies, the concentration of fluoroquinolone veterinary drug antibiotics in aquaculture wastewater is higher, and the composition of aquaculture wastewater is complex and the matrix interference is serious, which has a great impact on the determination results. The direct use of aquaculture wastewater as a sample for the determination of fluoroquinolone veterinary drugs is currently still There is no applicable method, so it is necessary to establish a sample pretreatment and content determination method for fluoroquinolone veterinary drug antibiotics in aquaculture wastewater, which can be used for the detection of actual aquaculture wastewater

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