The invention provides an injected amino acid derivative micromolecule gel material and application thereof, and belongs to the field of biomedical engineering in material chemistry. A preparation method comprises the following steps that first, phenylalanie methyl ester (D/L type) is regarded as a reaction zymolyte, and is modified by 1,4-naphthalic acid (NDC) and diethylene glycol, and amino acid derivative micromolecule gelator NDC-L or / and D-PhOMe, NDC-L or / and D-PhOH; and then, the gelator is added into polyethylene glycol, and heated to be completely dissolved, then equivoluminal deionized water is added, and after mixing, the injected amino acid derivative micromolecule gel material is obtained for several-minute standing. According to the injected amino acid derivative micromolecule gel material and the application thereof, a procedure is relatively simple, operation is convenient, the prepared gel material after freezing and drying is of a three-dimension (or nostoc commune) structure, biocompatibility is good, degradation is easy, the slow-release effect of medicine vitamin B12 is excellent, the gel material can be regarded as a medicine support or vector used for clinic treatment, and great practical prospects are achieved.