Device for pretreating enrofloxacin sample in aquaculture waste water as well as content measuring method

Abstract

The invention discloses a method for pretreating enrofloxacin in aquaculture waste water and measuring the content of the enrofloxacin, and belongs to the technical fields of analysis and detection oftrace organic matters in water environments. An aquaculture waste water pretreatment device is provided, and comprises a filtering and purifying column and a solid phase extraction column which are connected in series; the content measuring method comprises pretreatment, enrichment, elution and HPLC-MS / MS detection; and the veterinary medicine antibiotic enrofloxacin in the aquaculture waste water can be detected. The filtering and purifying column is connected in series with the solid phase extraction column, an interference substance on the measuring result of the veterinary medicine antibiotic enrofloxacin in a substrate is removed, and the pretreatment process is simplified. The self-filling type filtering and purifying column is used for automatically filling filtering fillers and the column body can be repeatedly used, so that the economic cost is reduced. The method for measuring the content of the veterinary medicine antibiotic enrofloxacin sample in the aquaculture waste water is established, so that the veterinary medicine antibiotic enrofloxacin can be separated and detected rapidly within short time, and the advantages of high operability and high efficiency are achieved.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of trace organic matter in water environment, in particular to a method for sample pretreatment and content determination of enrofloxacin in aquaculture wastewater. Background technique [0002] At present, veterinary antibiotics are widely used worldwide to treat animal diseases and promote healthy growth of animals. With the development of intensive farming in the livestock and poultry industry, veterinary antibiotics have been abused in large quantities. Animals have limited absorption of antibiotics, and about 75% of antibiotics are excreted in the feces and urine in the form of the original drug or metabolites. Many research results have shown that the sewage discharged from farms contains a large amount of veterinary drug antibiotic residues, and aquaculture wastewater is an important source of antibiotics in environmental water bodies. [0003] Enrofloxacin is a third-gener...

AI Technical Summary

Problems solved by technology

[0005] Chinese Patent Publication No. CN106370748A discloses the assay method and sample pretreatment method of fluoroquinolones in organic fertilizers; Determination method of class antibiotics; Chinese Patent Publication No. CN104730168A discloses a method for synchronous detection of residual tetracyclines, fluoroquinolones and sulfonamide antibiotics in environmental water, but there is no relevant report for the determination method of veterinary drug antibiotic enrofloxacin in aquaculture wastewater

Moreover, the composition of the aquaculture wastewater is complex, mainly including urine, residual feces, feed residues, washing water, etc., and sometimes domestic sewage generated during the production process. In addition, the aquaculture wastewater has a high concentration of organic matter, a large amount of suspended matter, deep color, and ammonia nitrogen content. High and contains a large number of bacteria, the matrix interferes seriously, and has a great impact on the measurement results

There is currently no applicable method for the determination of enrofloxacin directly using aquaculture wastewater as a sample, so it is necessary to establish a method for the determination of the content of veterinary drug antibiotic enrofloxacin in aquaculture wastewater, which can be used for the detection of actual aquaculture wastewater

Images