Preparation method of high-purity porous spherical silica gel filler

Abstract

The invention discloses a preparation method of a high-purity porous spherical silica gel filler, comprising the following preparation steps: purification of raw material tetraethoxysilane, synthesisand post-treatment of polyethoxysilane, synthesis of the high-purity spherical silica filler, and washing aging, roasting and drying. The invention provides the preparation method of the high-purity porous spherical silica gel filler. The spherical silica gel prepared by the preparation method has good regularity, and the metal impurity content in the porous high-purity silica gel is determined byusing an ICP full spectrum direct reading plasma emission spectrometer, and is as follows: Al: 3.5 mg / kg, Ca: 1 mg / kg, Fe: 1.5 mg / kg, Mg: 2.0 mg / kg, and Na: 1 mg / kg. The metal impurity content is lower than that of domestic silica gel, and the chromatographic performance is good. Under normal-phase and reversed-phase conditions, the column efficiency is about 65,000, and the peak shape is symmetrical. The high-purity porous spherical silica gel filler can be widely used as a chromatographic filler matrix for rapid separation of chemical or biological macromolecules.

Description

technical field [0001] The invention relates to the technical field of silica gel adsorption packing, in particular to a preparation method of high-purity porous spherical silica gel packing. Background technique [0002] Silica gel is an inorganic matrix filler. The main chemical composition is silica. The structure of silica gel is extremely stable. It is widely found in minerals such as sand and granite. Natural silica exists in the form of crystalline and amorphous structures. In addition to the very high mechanical strength, good filling stability and excellent chemical stability of silica gel, the surface of silica gel has special surface chemical characteristics. The surface of silica gel is also rich in silanol, and the silicon atoms exposed on the surface of silica gel tend to For tetrahedral ligands with oxygen atoms, the coordination is usually completed by combining monovalent hydroxyl groups, that is, the formation of surface silanol groups. The existence of sil...

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Problems solved by technology

However, due to the existence of impurities such as silanol and metal ions that often remain in silica gel, the surface chemical properties of silica gel are affected. The metal impurities in the silica gel structure usually affect the acidity of silanol, forming a heterogeneous structure, and chelating with the system. Complexation occurs, even if pickling is performed twice, it is difficult to avoid the interference of metal impurities, especially in the process of bonding, this effect makes the bonding efficiency not high, and some metals may become small The active point or catalytic point of the filler matrix can produce catalytic effect and non-specific adsorption

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