Process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin

A technology of polyamide resin and tea polyphenols, which is applied in the direction of organic chemistry, can solve the problems of long production cycle, large amount of solvent, and difficult separation, and achieve high and stable purification and extraction rate, simple and convenient separation method, and excellent chromatographic performance Effect

Active Publication Date: 2020-05-08
海山都(上海)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, enterprises that carry out deep processing of tea and use tea polyphenols as raw materials for products have sprung up like bamboo shoots after rain. However, the quality of products produced by most enterprises is not high, and enterprises with strong competitive advantages such as product quality, scale, and cost are not. There are not many products that can apply high-purity and low-impurity tea polyphenols active substances to health care products. In this regard, many companies or researchers have made a lot of efforts in the extraction and purification of tea polyphenols. Research and exploration, but there are problems such as low extraction efficiency, poor purity; difficult separation, cumbersome methods; large amount of solvent, long production cycle; large investment in equipment, etc.
In addition, in the existing technology of extracting caffeine, chloroform is basically used for extraction, but chloroform has the adverse effect of causing cancer. Using chloroform as an extraction agent will affect the health effects of tea polyphenols, and some studies have shown that tea polyphenols The higher the content, the lighter the color, and the tea polyphenol product obtained after extraction with chloroform is brown, and the purity of the product is low
In addition, the tea polyphenols extracted in the traditional process may also have the problem of excessive heavy metal ions
These existing technical problems have greatly affected the quality of tea polyphenols and greatly limited the application of tea polyphenols

Method used

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  • Process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin
  • Process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin
  • Process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin

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Embodiment 1

[0025] A process for extracting and purifying high-quality tea polyphenols with a spherical polyamide resin, comprising the following method steps:

[0026] Extraction: crush the dried tea leaves through a 0.5mm sieve, extract with hot water for 1-1.5h, the number of extractions is three times, the water temperature is 80°C, and the solid-to-liquid ratio is W:V=5;

[0027] Filtration: The extract is passed through a plate-and-frame filter, a microporous filter, and an ultrafilter respectively. The mesh number of the plate-and-frame filter is 400 mesh, and the aperture of the microporous filter is 0.5um. The molecular weight is 100,000, and the tea extract fine filtrate is obtained by solid-liquid separation;

[0028] Chromatography: pump the fine filtrate of the tea extract obtained by filtration into a chromatographic column equipped with HST-PA spherical polyamide chromatographic resin at a flow rate of 3CV / h for chromatographic adsorption. The eluent washes away caffeine a...

Embodiment 2

[0031] A process for extracting and purifying high-quality tea polyphenols with a spherical polyamide resin, comprising the following method steps:

[0032] Extraction: crush the dried tea leaves through a 0.5mm sieve, extract with hot water for 1-1.5h, the number of extractions is three times, the water temperature is 90°C, and the solid-to-liquid ratio is W:V=6;

[0033] Filtration: The extract is passed through a plate-and-frame filter, a microporous filter, and an ultrafilter respectively. The mesh number of the plate-and-frame filter is 400 mesh, and the aperture of the microporous filter is 0.5um. The molecular weight is 100,000, and the tea extract fine filtrate is obtained by solid-liquid separation;

[0034] Chromatography: pump the fine filtrate of the tea extract obtained by filtration into a chromatography column equipped with HST-PA spherical polyamide chromatographic resin at a flow rate of 4CV / h for adsorption. The citric acid water has a pH value of 2.5, the e...

Embodiment 3

[0037] A process for extracting and purifying high-quality tea polyphenols with a spherical polyamide resin, comprising the following method steps:

[0038] Extraction: crush the dried tea leaves through a 0.5mm sieve, extract with hot water for 1-1.5h, the number of extractions is three times, the water temperature is 100°C, and the solid-to-liquid ratio is W:V=10;

[0039] Filtration: The extract is passed through a plate-and-frame filter, a microporous filter, and an ultrafilter respectively. The mesh number of the plate-and-frame filter is 400 mesh, and the aperture of the microporous filter is 0.5um. The molecular weight is 100,000, and the tea extract fine filtrate is obtained by solid-liquid separation;

[0040] Chromatography: Pump the fine filtrate of the tea extract obtained by filtration into a chromatography column equipped with HST-PA spherical polyamide chromatographic resin at a flow rate of 3CV / h for adsorption. The citric acid water has a pH value of 4.0, the...

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Abstract

The invention discloses a process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin. The process comprises the following steps: extracting and filtering tea leaves, loading the filtered tea extract solution into a chromatographic column filled with spherical polyamide chromatographic resin for chromatography, eluting caffeine and a pigment by using a citric acid aqueous solution as an eluent, then eluting tea polyphenol by using ultrapure water and finally eluting tea polyphenol by using an ethanol aqueous solution as a mobile phase, and finally, concentrating and crystallizing the collected tea polyphenol eluent to obtain a colorless and transparent high-quality tea polyphenol crystal finished product. According to the process for extracting and purifying high-quality tea polyphenol by using spherical polyamide resin, the extraction method is simple, eco-friendly, free of pollution and low in production cost, the requirements that the purity of the tea polyphenol is larger than 99% and the extraction rate is larger than 90% can be met only through one-step chromatographic purification, the prepared tea polyphenol is high in yield, high inextraction rate and high in quality, the requirements of more fields can be met, and therefore wider market requirements are met.

Description

technical field [0001] The invention relates to the technical field of extracting and purifying natural product active substances, in particular to a process for extracting and purifying high-quality tea polyphenols with a spherical polyamide resin. Background technique [0002] Tea polyphenols is the general term for polyphenols in tea, including flavanols, anthocyanins, flavonoids, flavonols, and phenolic acids. Tea polyphenols are one of the main components that form the color and aroma of tea, and are also one of the main components that have health care functions in tea. Tea polyphenols have a wide application value and are widely used in medicine, daily cosmetics, food and other fields. Studies have shown that the active substances of tea polyphenols have many unique functions such as scavenging free radicals, detoxification, anti-aging, anti-cancer, and lowering blood lipids; they also have anti-radiation effects, which can effectively prevent radioactive substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G99/00
CPCC07G99/00
Inventor 厉留柱宁勇
Owner 海山都(上海)生物技术有限公司
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