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Vector material for immobilization of cellulase and preparation method of vector material

A technology of cellulase and carrier materials, applied in the direction of immobilization on or in inorganic carriers, immobilization on/in organic carriers, biochemical equipment and methods, etc., can solve the problem of intense reaction, loss of enzyme activity, enzyme Problems such as low leakage rate

Inactive Publication Date: 2017-12-01
苏州凯邦生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The covalent binding method is to immobilize the enzyme on a suitable carrier through a covalent bond method. The enzyme and the carrier produce a chemical interaction, the binding force is strong, the enzyme leakage rate is low, and it has good stability and recycling performance. Intense, easy to lose enzyme activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method for a carrier material for cellulase immobilization, comprising the following steps:

[0024] (1) Dissolve ethyl silicate in ethanol solution, add nano-Fe 3 o 4 Ultrasonic dispersion of particles for 20min, nano-Fe 3 o 4 The volume ratio of the used mass of particles to ethyl silicate is 30g / L, and then a mixture of glacial acetic acid and absolute ethanol is added dropwise during vigorous stirring. After the dropwise addition is completed, continue stirring for 4 hours, then let it stand for 60 hours, and dry it. Nano-SiO obtained after grinding 2 / Fe 3 o 4 composite materials;

[0025] (2) Dissolve polyacrylonitrile in DMSO, add nano-SiO 2 / Fe 3 o 4 Composite material, polyacrylonitrile and nano-SiO 2 / Fe 3 o 4 The mass ratio of the composite material is 4:1, ultrasonic dispersion is performed for 1 hour, a silane coupling agent is added to stir and mix evenly, and then sprayed to obtain polyacrylonitrile composite microspheres. The pa...

Embodiment 2

[0030] A preparation method for a carrier material for cellulase immobilization, comprising the following steps:

[0031] (1) Dissolve ethyl silicate in ethanol solution, add nano-Fe 3 o 4 Ultrasonic dispersion of particles for 45 minutes, nano-Fe 3 o 4 The volume ratio of the used mass of particles to ethyl silicate is 45g / L, and then a mixture of glacial acetic acid and absolute ethanol is added dropwise during vigorous stirring. After the dropwise addition is completed, continue to stir for 5h, then stand still for 72h, and dry. Nano-SiO obtained after grinding 2 / Fe 3 o 4 composite materials;

[0032] (2) Dissolve polyacrylonitrile in DMSO, add nano-SiO 2 / Fe 3 o 4 Composite material, polyacrylonitrile and nano-SiO 2 / Fe 3 o 4 The mass ratio of the composite material is 4:1, ultrasonic dispersion is performed for 2 hours, a silane coupling agent is added to stir and mix evenly, and then sprayed to obtain polyacrylonitrile composite microspheres. The particle si...

Embodiment 3

[0037] A preparation method for a carrier material for cellulase immobilization, comprising the following steps:

[0038] (1) Dissolve ethyl silicate in ethanol solution, add nano-Fe 3 o 4 Ultrasonic dispersion of particles for 20min, nano-Fe 3 o 4 The volume ratio of the used mass of particles to ethyl silicate is 45g / L, and then a mixture of glacial acetic acid and absolute ethanol is added dropwise during vigorous stirring. After the dropwise addition is completed, continue stirring for 4h, then stand still for 72h, and dry. Nano-SiO obtained after grinding 2 / Fe 3 o 4 composite materials;

[0039] (2) Dissolve polyacrylonitrile in DMSO, add nano-SiO 2 / Fe 3 o 4 Composite material, polyacrylonitrile and nano-SiO 2 / Fe 3 o 4 The mass ratio of the composite material is 4:1, ultrasonic dispersion is performed for 1 hour, a silane coupling agent is added to stir and mix evenly, and then sprayed to obtain polyacrylonitrile composite microspheres. The particle size of...

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Abstract

The invention discloses a vector material for immobilization of cellulase and a preparation method of the vector material. The preparation method comprises steps as follows: prepared polyacrylonitrile composite microspheres are added to DMAC (dimethylacetamide), soaked for 4-6 h, uniformly mixed with lithium chloride and then mixed with chitin and a catalyst uniformly, nitrogen is introduced, and the mixture is stirred at the room temperature for 2-4 h, then heated, stirred to react at the temperature of 100-120 DEG C for 18-24 h, cooled to the room temperature, washed with absolute ethanol and deionized water sequentially, vacuumized and dried. The vector material has good mechanical strength, better stability and relatively high binding force with enzymes and is longer in service life due to the fact that enzyme molecules cannot easily fall from a vector in use. The invention further provides a use method of the vector material for immobilization of the cellulase.

Description

technical field [0001] The invention relates to the field of enzyme preparations, in particular to a carrier material for cellulase immobilization and a preparation method thereof. Background technique [0002] Cellulase is a multi-component complex enzyme system, mainly including exo-β-glucanase, endo-β-glucanase and β-glucosidase. degraded into glucose. Most of the components of cellulase are glycoproteins, and the sugar groups in the structure have a certain protective effect on cellulase from proteolysis. The molecular structures and folding methods of cellulase from different sources are different, and their generality is generally composed of three parts: the spherical catalytic active region with catalytic function, the binding region with the function of binding cellulose, and the connecting region. A highly glycosylated peptide acid at one end. Current research suggests that for effective enzymatic hydrolysis of cellulose materials, cellulase must first be adsorb...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08B01J13/06B01J13/04
CPCB01J13/04B01J13/043B01J13/06C12N9/2437C12N9/2445C12N11/08C12N11/14C12Y302/01004C12Y302/01021C12Y302/01091
Inventor 冯海燕
Owner 苏州凯邦生物技术有限公司
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