Quantitative detection kit of hepatitis B virus (HBV) nucleic acid
A hepatitis B virus, detection kit technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as poor accuracy and low sensitivity, and achieve simple operation, low cost and good repeatability. Effect
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[0023] Real-time quantitative PCR detection example of HBV DNA
[0024] (1) Reagent preparation
[0025] a. Preparation of PCR reaction solution
[0026] The PCR buffer composition is 30mmol / L Tris-HCl (PH8.3), 10mmol / L (NH4) 2 SO 4 , 30mmol / LKCl, 4.5mmol / LMgCl 2 , 0.15mmol / L dNTPs. The concentration of the upstream primer and downstream primer for amplifying the target gene is 20 pmol, the concentration of the probe for target gene detection is 8 pmol, the concentration of the upstream primer and downstream primer of the internal standard is 8 pmol, and the concentration of the internal standard probe is 8 pmol.
[0027] b. Take the corresponding amount of Lysis Conjugate Solution and Internal Standard according to the proportion (400 μl of Lysis Conjugate Solution per person + 0.1 μl of internal standard per person), mix well to form Lysis Conjugate Solution-mix, centrifuge briefly and set aside.
[0028] c. According to the amount of the sample to be tested, negative c...
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