High-accuracy quantitative detection method and kit for HIV-1 nucleic acid

Abstract

The invention provides a high-accuracy quantitative detection kit for HIV-1 nucleic acid. The kit is applied to the field of biomedical clinical diagnosis. The gene amplification locus of the kit is positioned in the 5'LTR conserved region of an HIV genome; the kit comprises a nucleic acid extraction kit based on a magnetic bead method and an HIV nucleic acid amplification kit, wherein the nucleic acid extraction kit based on the magnetic bead method comprises a lysed combined liquid, a rinsing liquid A, a rinsing liquid B, a rinsing liquid C, eluant and a magnetic bead liquid; the HIV nucleic acid amplification kit comprises an RT-PCR reaction liquid, an enzyme mixed liquid, an HIV-interior standard, an HIV quantitative reference product 1-4, a negative quality control product, a critical positive quality control product and a strong positive quality control product. The kit provided by the invention is simple and quick to operate, low in cost, high in detection sensitivity, good in repeatability, high in primer and probe gene type coverage rate, high in conservative property and strong in specificity, an efficient internal standard system is introduced, and the problems of mutual inhibition and interference due to the simultaneous amplification of a target gene and the internal standard are solved.

Description

technical field [0001] The invention relates to a method for highly precise nucleic acid quantitative detection of AIDS (HIV-1) in the field of biomedical clinical diagnosis and a kit thereof. Background technique [0002] Human immunodeficiency virus (HIV) is a pathogenic microorganism that causes a chronic fatal disease AIDS (Acquired immune deficiency syndrome, AIDS). Because AIDS is contagious and highly lethal, it has become one of the major diseases that have been focused on prevention and control worldwide. Since the first cases of AIDS were first reported in 1981, AIDS has killed more than 25 million people so far. Since the first AIDS case was discovered in China in 1985, the prevalence of AIDS has been on the rise. In recent years, the number of AIDS infections and morbidity in China has also increased rapidly. The current situation of AIDS in China, according to the data shown by the United Nations agency in China, there are currently about 840,000 people livin...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method and kit for highly accurate nucleic acid quantitative detection of AIDS (HIV-1), which fundamentally solves the problems of incomplete genotype coverage and missed detection in the existing HIV-1 nucleic acid quantitative PCR technology The primers and TaqMan fluorescent probes are located in the 5'LTR region of the HIV genome. The primers and probes have a wide range of genotype coverage, high detection sensitivity, strong specificity, and stability Good sex and other advantages

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