Reagent kit of detecting human immunodeficiency virus type 1 by fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)

A human immunodeficiency, fluorescent quantitative technology, applied in the field of fluorescent quantitative RT-PCR detection kits for human immunodeficiency virus type 1, can solve problems such as incurable, no effective vaccine, etc., to reduce false negative results and avoid false negatives and false positives, improving reliability and accuracy

Inactive Publication Date: 2013-04-17
DAAN GENE CO LTD
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even with the development of medical technology, the spread of AIDS has been effective

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent kit of detecting human immunodeficiency virus type 1 by fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)
  • Reagent kit of detecting human immunodeficiency virus type 1 by fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)
  • Reagent kit of detecting human immunodeficiency virus type 1 by fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Detection method of human immunodeficiency virus type 1 kit

[0039] (1) Specimen collection, transportation and preservation

[0040] Specimen collection:

[0041] Serum-Use a disposable sterile syringe to draw 2 ml of the subject's venous blood, inject it into a sterile dry glass tube, and place it at room temperature (22~25℃) for 30~60 minutes. The blood sample can spontaneously and completely agglutinate and separate the serum, or use it directly Centrifuge at 1500rpm for 5 minutes in a horizontal centrifuge; aspirate the upper layer of serum and transfer it to a 1.5ml sterile centrifuge tube.

[0042] Plasma-Use a disposable sterile syringe to draw 2 ml of the subject's venous blood, and inject it into a glass tube containing EDTA-2Na (disodium ethylenediaminetetraacetic acid) or sodium citrate anticoagulant, and then gently invert the glass tube to mix immediately 5-10 times, fully mix the anticoagulant and venous blood. After 5-10 minutes, the plasma can be s...

Embodiment 2

[0081] Example 2: Use of quality control materials and positive quantitative reference materials in the human immunodeficiency virus type 1 kit

[0082] The quality control materials and positive quantitative reference materials in the human immunodeficiency virus type 1 kit include HIV-1 strong positive quality control materials, HIV-1 borderline positive quality control materials, negative control materials and HIV-1 positive quantitative reference materials 1- 4. Used for quality control in clinical trials, the operation method is the same as the sample to be tested.

[0083] Quality Control:

[0084] Negative control: FAM detection pathway amplification curve has no logarithmic growth period, VIC detection pathway amplification curve is logarithmic growth period;

[0085] HIV-1 positive quality control products: the FAM detection pathway amplification curve has a significant logarithmic growth period, showing a typical S-type amplification curve, and the HIV-1 strong positive qual...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a reagent kit of detecting human immunodeficiency virus type 1 by fluorescence quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and in particular relates to a reagent kit of rapidly and quantitatively detecting infection of human immunodeficiency virus type 1 by one-step real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR). The reagent kit is high in sensitivity and specificity, is used for quantitatively detecting human immunodeficiency virus type 1 nucleic acid RNA (Ribonucleic Acid) in a serum or plasma sample and is suitable for auxiliary diagnosis of the infection of the human immunodeficiency virus type 1 and curative effect survey of drug therapy of a human immunodeficiency virus type 1 infected person.

Description

Technical field [0001] The invention relates to a kit for detecting human immunodeficiency virus type 1 by fluorescent quantitative RT-PCR, in particular to a one-step real-time fluorescent reverse transcription-polymerase chain reaction (RT-PCR) technique for rapid and quantitative detection of human immunodeficiency virus Kit for type 1 infection. The kit has high sensitivity and specificity. The kit of the invention can realize the quantitative detection of human immunodeficiency virus type 1 nucleic acid RNA in serum or plasma samples, and is suitable for auxiliary diagnosis and diagnosis of human immunodeficiency virus type 1 infection. Monitoring the efficacy of drug therapy in patients with human immunodeficiency virus type 1 infection. Background technique [0002] Human immunodeficiency virus (HIV) is the pathogen of acquired immunodeficiency syndrome (AIDS). HIV is the first new retrovirus isolated from the blood of a male homosexual patient with chronic lymphadenopat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 李明丁渭陈华云
Owner DAAN GENE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap