Reagent kit for extracting virus DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) by using paramagnetic particle method and use method of reagent kit

A technology of kit and magnetic bead method, which is applied in the field of molecular biology, can solve the problems of high price, achieve low cost, complete DNA or RNA fragments, and realize the effect of automatic nucleic acid extraction

Inactive Publication Date: 2013-07-24
福州泰普生物科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although nucleic acid isolation technology has been greatly developed at present, there are some shortcomings: for example,
These kits have

Method used

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  • Reagent kit for extracting virus DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) by using paramagnetic particle method and use method of reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the kit that adopts magnetic bead method to extract viral DNA or RNA, it comprises lysate, cleaning solution 1, cleaning solution 2, cleaning solution 3, eluent and magnetic bead suspension; Wherein,

[0053] The preparation method of the lysate is: weigh 472.6g guanidine isothiocyanate, 12.1g Tris, dissolve with deionized water, adjust the pH to 6.0~6.8, add 70ml Triton X-100, 20ml 0.5M EDTA-2Na and 50ml Acryl Carrier, after mixing, dilute to 1000ml with deionized water.

[0054] The configuration method of cleaning solution 1 is: weigh 382.2g of guanidine hydrochloride and 29.4g of sodium citrate, dissolve them in deionized water, adjust the pH to 6.0~6.8, add 350ml of absolute ethanol, mix well, and rinse with deionized water Dilute to 1000ml.

[0055] Cleaning solution 2: ethanol aqueous solution with a volume fraction of 75%;

[0056] Cleaning solution 3: 0.01 mol / L Tris aqueous solution, pH value is 4.0~5.0;

[0057] Eluent: deionized water;

[0...

Embodiment 2

[0059] Embodiment 2, a kit for extracting viral DNA or RNA using the magnetic bead method, which includes a lysate, a cleaning solution 1, a cleaning solution 2, a cleaning solution 3, an eluent and a magnetic bead suspension; wherein,

[0060] The preparation method of the lysate is: weigh 716.5g guanidine hydrochloride, 14.7g sodium citrate, dissolve with deionized water, adjust the pH to 6.0~6.8, add 150ml Triton X-100, 20ml 0.5M EDTA-2Na and 50ml Acryl Carrier, after mixing, dilute to 1000ml with deionized water.

[0061] The configuration method of cleaning solution 1 is: weigh 590.1g guanidine isothiocyanate and 6.1g Tris, dissolve with deionized water, adjust the pH to 6.0~6.8, add 600ml of absolute ethanol, mix well, and use deionized Dilute water to 1000ml.

[0062] Cleaning solution 2: ethanol aqueous solution with a volume fraction of 75%;

[0063] Cleaning solution 3: 0.03 mol / L Tris aqueous solution, pH value is 4.0~5.0;

[0064] Eluent: deionized water;

[00...

Embodiment 3

[0066] Embodiment 3, the kit that adopts magnetic bead method to extract viral DNA or RNA, it comprises lysate, cleaning solution 1, cleaning solution 2, cleaning solution 3, eluent and magnetic bead suspension; Wherein,

[0067] The preparation method of the lysate is: weigh 590.5g guanidine isothiocyanate, 23.5g sodium citrate, dissolve with deionized water, adjust the pH to 6.0~6.8, add 100ml Triton X-100, 20ml 0.5M EDTA-2Na and 50ml Acryl Carrier, after mixing, dilute to 1000ml with deionized water.

[0068] The configuration method of cleaning solution 1 is: weigh 447.6g of guanidine hydrochloride and 9.7g of Tris, dissolve them in deionized water, adjust the pH to 6.0~6.8, add 500ml of absolute ethanol, mix well, and dilute to volume with deionized water to 1000ml.

[0069] Cleaning solution 2: ethanol aqueous solution with a volume fraction of 75%;

[0070] Cleaning solution 3: 0.02 mol / L Tris aqueous solution, pH value is 4.0~5.0;

[0071] Eluent: deionized water; ...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a reagent kit for extracting virus DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) based on a magnetic bead method and a use method of the reagent kit. The reagent kit comprises lysate, a washing liquid 1, a washing liquid 2, a washing liquid 3, an eluent and a magnetic bead suspension. A buffer solution system of the reagent kit can remove protein, pigment, greases and other inhibitory impurities in specimens to the largest extent by matching with a special nanometer magnetic beads, so that the extracted DNA or RNA has the advantages of complete fragment, high yield, high purity, stability and reliability and low cost.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a kit for extracting viral DNA or RNA by a magnetic bead method and a use method thereof. Background technique [0002] Nucleic acid research is an important topic in modern biology and medical research. As the carrier of genetic information, nucleic acid is the material basis of gene expression. In addition to playing a very important role in the normal growth, development, and reproduction of organisms, it is also related to abnormal conditions of life, such as tumor occurrence, radiation, etc. Injuries, genetic diseases, etc. are also closely related. Therefore, nucleic acid is an important topic in modern biology and medical research. Whether it is research on the structure and function of nucleic acids, or research on genetic engineering, protein engineering, etc., it is first necessary to separate and purify nucleic acids. [0003] In the early days...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 李旭新杨玉芬
Owner 福州泰普生物科学有限公司
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