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Biosensor for organophosphorus pesticide rapid detection and detecting method thereof

A rapid technology for organophosphorus pesticides, applied in the direction of material electrochemical variables, etc., can solve the problems of unfavorable primary screening work, etc., and achieve the effects of narrowing the quantitative detection range, simple preparation, and small diffusion barriers

Inactive Publication Date: 2014-03-26
CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for biosensors that immobilize enzymes on electrodes, substrate solutions need to be prepared for each detection, which is not conducive to rapid on-site preliminary screening.

Method used

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  • Biosensor for organophosphorus pesticide rapid detection and detecting method thereof
  • Biosensor for organophosphorus pesticide rapid detection and detecting method thereof
  • Biosensor for organophosphorus pesticide rapid detection and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: A method for preparing a glassy carbon electrode immobilized with poly-m-phenylenediamine tyrosinase (Tyr) and substrate (sodium 1,2-naphthoquinone-4-sulfonate)

[0054] A kind of preparation method of the glassy carbon electrode of immobilizing tyrosinase (Tyr) and substrate (1,2-naphthoquinone-4-sodium sulfonate) with poly-m-phenylenediamine is as follows:

[0055] a. Polish the glassy carbon electrode with a diameter of 2mm (used as a working electrode) with alumina polishing powder with a particle diameter of 1μm, 0.3μm and 0.05μm in sequence, and then use distilled water, ethanol, and distilled water to ultrasonically clean it for 3 minutes to clean it up after drying;

[0056] b. Drop-coat the newly prepared 10 μL 0.05mol / L sodium 1,2-naphthoquinone-4-sulfonate solution on the surface of the bare glassy carbon electrode obtained in step a, and let it dry naturally;

[0057] c. Drop-coat 10 μL of 20 mg / mL tyrosinase and 5 μL of 2.5 mg / mL glutaraldehyde ...

Embodiment 2

[0060] Example 2: A biosensor for rapid detection of organophosphorus pesticides

[0061] A biosensor for rapid detection of organophosphorus pesticides is called GC-NQS-Tyr-1,3-DAB biosensor. The sensor uses a saturated calomel electrode (SCE) as a reference electrode, and a platinum sheet electrode as a counter electrode. On the glassy carbon electrode that has tyrosinase (Tyr) and substrate (1,2-naphthoquinone-4-sodium sulfonate) immobilized with polym-phenylenediamine prepared in embodiment 1 as working electrode, this working electrode Tyrosinase (Tyr) and substrate (NQS) were electropolymerized and immobilized on the glassy carbon electrode with poly-m-phenylenediamine, a non-conductive polymer.

Embodiment 3

[0062] Embodiment 3: a kind of organophosphorus pesticide rapid detection method,

[0063] A rapid detection method for organophosphorus pesticides. In the method, the aforementioned biosensors for rapid detection of organophosphorus pesticides are put into phosphate buffer solution with pH=6.5, and chronoamperometry is used to scan with current-time method on an electrochemical workstation to study tyrosine The electrochemical behavior of the acidase glassy carbon electrode comprises the following steps:

[0064] First, measure the blank curve before adding organophosphorus pesticide, which is in 30mL0.05mol / L phosphate buffer (passed through O 2 15min) to scan and stabilize at E=+0.10V for 90s (at this time, the substrate 1,2-naphthoquinone-4-sulfonic acid sodium (NQS) becomes 1,2-naphthalenediol-4-sulfonic acid Sodium (H 2 NQS)); at E=-0.15V for 15s(H 2 NQS is converted to NQS); recovery 60s at E=+0.10V (NQS is converted to H 2 NQS);

[0065] After the blank curve is m...

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Abstract

The invention discloses a biosensor for organophosphorus pesticide rapid detection and a detecting method of the biosensor. A saturated calomel electrode is used as a reference electrode; a platinum plate electrode is a counter electrode; a glass carbon electrode fixed with tyrosinase and 1,2-naphthoquinone-4-sodium sulfonate substrate is used as a working electrode; the working electrode is prepared by electrically and chemically polymerizing and fixing the tyrosinase and the 1,2-naphthoquinone-4-sodium sulfonate substrate on the glassy carbon electrode with a non-conducting polymer. Therefore, the biosensor can detect the organophosphorus pesticide more reliably, sensitively and rapidly. The biosensor is simple to prepare, reusable, high in utilization rate, and suitable for field operation, can be used to detect the organophosphorus pesticide without preparation of enzyme and substrate solution every time, can screen out a large number of unqualified samples in short time, shortens the range of quantitative detection, and is an effective supplement for the complicated laboratory method.

Description

Technical field: [0001] The invention relates to the fields of food and agriculture, in particular to a biosensor for rapid detection of organophosphorus pesticides and a detection method thereof. Background technique: [0002] With the development of agricultural industrialization, the production of agricultural by-products is increasingly dependent on exogenous substances such as pesticides, hormones and antibiotics. The amount of pesticides used in food, fruits, vegetables, and tea remains high in China, and the unreasonable use of these substances will inevitably lead to excessive pesticide residues in agricultural and sideline products, affecting the food safety of consumers. In recent years, human health has been seriously threatened due to problems caused by food safety, such as pesticide residues in fruits and vegetables due to excessive use or non-compliant use of pesticides, causing acute or chronic poisoning of people. At present, dozens of highly toxic pesticide...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 潘文龙柳亚玲陈泳杜志云李正全朱黎明韦文蔚叶汝汉仇镇武林晨尹冲
Owner CHINA NAT ANALYTICAL CENT GUANGZHOU
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