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Three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and putrescine and cadaverine detection method based on substrate

A three-dimensional flexible and detection method technology, applied in Raman scattering, material excitation analysis, etc., can solve problems such as lack of reliable solutions, achieve controllable morphology and structure, enhance SERS activity, and have good application prospects

Pending Publication Date: 2022-04-15
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In view of this, the preparation of PVDF-based SERS substrates for the detection of putrescine and cadaverine has good application prospects, but there is a lack of reliable solutions

Method used

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  • Three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and putrescine and cadaverine detection method based on substrate
  • Three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and putrescine and cadaverine detection method based on substrate
  • Three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and putrescine and cadaverine detection method based on substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] A three-dimensional flexible SERS substrate, which is prepared by the following method:

[0082] 1) Prepare clustered ZnO nanorods on PVDF membrane to obtain ZnO-P array substrate:

[0083] 1-1) Set the size to 0.6×0.6cm 2 The PVDF membrane was immersed in 25-30mM zinc acetate dihydrate to react for 10-20min, and then dried with nitrogen;

[0084] 1-2) heating the PVDF membrane obtained in step 1-2) at 140-150° C. for 4-5 hours;

[0085] 1-3) Immerse the PVDF membrane obtained in step 1-3) in a mixture of 25-30 mM urotropine and zinc nitrate hexahydrate, and heat at 80-90°C for 4-5 hours to obtain clustered cone-shaped oxidation Zinc nanorod array (ZnO / P), that is, ZnO-P array substrate. Scanning electron microscope observation was carried out to the ZnO-P array substrate obtained, the result is as follows figure 2 As shown in a, the diameters of the bottom and top are 70±5nm and 25±5nm, respectively, and the length is 730±50nm.

[0086] 2) Coating gold film on Zn...

Embodiment 2

[0107] A method for detecting putrescine and cadaverine is provided, which uses the MBA-Big-AuNP-Au-ZnO-P array substrate prepared in the embodiment combined with Raman signal detection to realize the determination of the total concentration of putrescine and cadaverine.

[0108] In this embodiment, the method directly detects the total concentration of putrescine and cadaverine in solid or liquid samples, specifically taking pork as an example for illustration, and the detection method includes the following steps:

[0109] S1, constructing a standard curve in advance, the standard curve characterizes the relationship between the Raman signal intensity and the total concentration of putrescine and cadaverine:

[0110] S1-1, using 10 -2 The standard amine solution of M is diluted to prepare a series of standard amine solutions with different concentrations;

[0111] S1-2. For each concentration of standard amine solution, first react with the MBA-Big-AuNP-Au-ZnO-P array subst...

Embodiment 3

[0117] The detection method for putrescine and cadaverine provided in this embodiment is to detect the total concentration of putrescine and cadaverine in the gas generated after the sample deteriorates. Similarly, pork is used as an example for illustration. The detection method includes the following step:

[0118] S1. Construct a standard curve in advance, which represents the relationship between the Raman signal intensity and the total concentration of putrescine and cadaverine; the specific steps are the same as in Example 2, and will not be repeated here.

[0119] S2. Put 5-10g of fresh pork in a centrifuge tube, heat at 37°C for 0.5-1h, and then deteriorate at room temperature for 1-5h; 4, 5 hours, also used the comparison sample (non-deteriorating treatment) at the same time;

[0120] S3. Provide a microfluidic device, wherein a microwell array is provided at the bottom of the sample pool of the microfluidic device;

[0121] S4. Place the MBA-Big-AuNP-Au-ZnO-P array s...

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Abstract

The invention discloses a three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and a putrescine and cadaverine detection method based on the substrate. The substrate is prepared by the following method: 1) preparing clustered ZnO nanorods on a PVDF (Polyvinylidene Fluoride) film; 2) coating the ZnO nanorod with a gold film; 3) after modification by polyethyleneimine, fixing gold nanoparticles on the surface of the ZnO nanorod; and (4) Au < 3 + > is reduced, AuNPs further grows, and the three-dimensional flexible SERS substrate is obtained. According to the preparation method, the 3D rose dendritic nanostructure is prepared through in-situ chemical growth of AuNPs on the surface of the ZnO nanorod, and the SERS activity of the heterostructure is remarkably enhanced through the LSPR effect and the CT effect; the p-MBA modified 3D rose dendritic SERS substrate can realize high detection sensitivity and rapid detection of putrescine and cadaverine in a sample, and has a good application prospect in instant detection of meat products.

Description

technical field [0001] The invention relates to the field of surface-enhanced Raman spectroscopy, in particular to a three-dimensional flexible SERS substrate and a detection method for putrescine and cadaverine based on the substrate. Background technique [0002] Putrescine and cadaverine are two typical toxic biogenic amines, mainly produced by microorganisms in spoilage meat products through decarboxylation of corresponding amino acids, which can cause serious health problems such as coughing, shortness of breath, nausea, vomiting, and even death . Therefore, the rapid, sensitive and quantitative detection of putrescine and cadaverine in meat products is of great significance. Traditional analytical methods such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) have high sensitivity and are also the "gold standard" for molecular quantitative analysis. However, these methods mainly rely on large-scale commercial and expens...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 张志强孙姣姣尹焕才刘聪黎海文张涛蒋克明周武平
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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