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Purification of cadaverine using high boiling point solvent

a high boiling point and solvent technology, applied in the field of purification methods of cadaverine, can solve the problems of high heating temperature, long evaporation/distillation time, and low recovery yield of cadaverine, and achieve the effects of low boiling point, low cadaverine recovery yield, and high boiling poin

Active Publication Date: 2017-05-18
CIBT AMERICA INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about a method to purify cadaverine from an aqueous solution containing impurities. The method involves mixing the solution with certain bases to create a new solution, and then distilling or evaporating the new solution to obtain pure cadaverine. One or more solvents are added to the evaporation / distillation system before the process stops, which includes high boiling point solvents. The technical effect of this invention is the ability to obtain pure cadaverine from an aqueous solution containing impurities.

Problems solved by technology

However, the cadaverine fermentation broth or enzymatic conversion solution may comprise involatile impurities that interfere with the cadaverine evaporation / distillation.
Thus, the cadaverine evaporation / distillation may require high heating temperature and long evaporation / distillation time, while the cadaverine recovery yield is still relatively low and may contain undesired contaminations.
Conventional bases such as sodium hydroxide, potassium hydroxide, sodium phosphate and sodium carbonate, on the one hand, are expensive, and on the other hand, lead to significant environmental burden due to the production of large amounts of soluble basic salts that make waste treatment difficult.

Method used

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  • Purification of cadaverine using high boiling point solvent
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  • Purification of cadaverine using high boiling point solvent

Examples

Experimental program
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example 1

Preparation of a Lysine Fermentation Broth

(1) LB Medium Slants:

[0128]LB medium slants were prepared using LB medium containing tryptone 1%, yeast extract 0.5%, and NaCl 1%, pH 7.2.

(2) Primary Seed Culture Medium (Shake Flask Seed Culture Medium)

[0129]A Corynebacterium culture expressing lysine was grown on the LB medium slants, and transferred into a 500 mL seed flask containing the 200 mL liquid medium (liquid broth medium containing beef extract 1%, tryptone 1%, yeast extract 0.5%, and NaCl 0.5%, pH 7.0), then cultured at 33° C. on 200 rmp shaking bed for 10-15 hours.

(3) Lysine Fermentation

[0130]A fermentation medium (5 L) was added to a 10 L fermentor. Then the seed liquid prepared above was transferred into the fermentor for fermentation. The fermentation medium contained: glucose 1.5%, molasses 0.2%, corn steep liquor 0.06%, soybean meal hydrolysate 0.05%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.01%, and magnesium sulfate 0.005%. After sterilization at 121° C. f...

example 2

Preparation of Lysine Decarboxylase (LDC)

(1) Seeds and Fermentation Medium:

[0132]Seed culture medium (g / L): Tryptone 10, beef extract 5, NaCl 5, corn steep liquor, 5, pH 7.2. A wild Hafnia alvei strain (Hafnia alvei 1.1009 from CGMCC, http: / / www.cgmcc.net / index.php / Contents / show / id / 460) expressing LDC was grown on slant, transferred to a triangle flask containing 30 mL seed medium, and cultured at 35° C. with shaking at 170 R / min for 15 hour.

(2) Culture Conditions

[0133]Fermentation medium (g / L): glucose 18, yeast extract 20, corn steep liquor 36.6, MgSO4 0.3, KH2PO4 0.1, NaCl 3, L-lysine 5, vitamin B6 1, and pH 6.5-7.0.

[0134]10% seed was transferred into a 250 mL flask containing 100 mL fermentation medium. In the cell growth phase, the temperature was controlled at 35° C. and the cells were cultured on a rotary shaker 200 r / min oscillation for 13 hours; then cultured statically for 5 hours. The obtained LDC fermentation broth was used directly in LDC catalyzed conversion of lysine ...

example 3

LDC Catalyzed Conversion of Lysine

(1) Enzymatic Conversion of Lysine Hydrochloride

[0135]100 L LDC fermentation broth (Example 2) was added in a 250 L reactor, acetic acid and sodium acetate were added to the reaction to final molar concentrations of 0.2 mol / L, respectively. Commercially available lysine hydrochloride was added to the reaction to control the lysine concentration at 3 g / L, and Tween-80 (total 0.15 kg) was added to the reaction. The reaction was stirred at 35° C. The reaction was complete within about 5 hours, with a lysine molar conversion rate of about 98%. The enzymatic conversion s was concentrated to obtain a cadaverine salt solution (about 5%), which was ready for optional further processing.

(2) Enzymatic Conversion of Lysine Sulfate

[0136]100 L LDC fermentation broth (Example 2) was added in a 250 L reactor, acetic acid and sodium acetate were added to the reaction to final molar concentrations of 0.2 mol / L, respectively. Commercially available lysine sulfate (65...

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Abstract

The present invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition comprising one or more involatile impurities, the method comprising: (1) mixing the aqueous cadaverine salt composition with one or more bases selected from the group consisting of Ca(OH)2, Mg(OH)2, CaO and MgO to provide an aqueous cadaverine composition; and (2) distilling or evaporating the aqueous cadaverine composition to produce purified cadaverine; wherein one or more solvents are added to the evaporation / distillation system before the evaporation / distillation stops, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation-in-Part of co-pending U.S. Ser. No. 14 / 764,109, filed on Jul. 28, 2015, a 35 U.S.C. §371 National Phase Entry Application of PCT / CN2013 / 071045. filed on Jan. 28, 2013, designating the United States and entitled “Purification of Cadaverine Using High Boiling Point Solvent”. The entire disclosure of each application is herein incorporated by reference.TECHNICAL FIELD OF THE INVENTION[0002]The invention relates to methods for purification of cadaverine from aqueous cadaverine salt compositions. More specifically, the invention relates to methods for purification of bio-based cadaverine from bio-based productions thereof.BACKGROUND[0003]Bio-based cadaverine is a valuable platform chemical involved in the production of partially or fully bio-based products, such as partially or fully bio-based Nylon 56 and Nylon 510. Bio-based cadaverine can be synthesized via lysine decarboxylation of lysine in microorganism...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07C209/84
CPCC07C209/84C07C211/09
Inventor LIU, XIUCAILIU, CHARLIEDAI, DUANFANGQIN, BINGBINGLI, NAIQIANG
Owner CIBT AMERICA INC
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