Apparatus for continuous production and extraction of cadaverine via immobilized cells

A technology for immobilized cells and extraction of pentamethylenediamine, which is applied in the field of continuous production and extraction of pentamethylenediamine by immobilized cells, and in the field of compound production and extraction devices, can solve the problems of complex separation, high cell toxicity, and non-reusable cells. Achieve stable product quality, high equipment utilization, and high production efficiency

Active Publication Date: 2017-02-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional whole cell transformation to produce pentamethylenediamine with high concentration is more toxic to cells, the cells cannot be reused, and the separation is more complicated

Method used

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  • Apparatus for continuous production and extraction of cadaverine via immobilized cells
  • Apparatus for continuous production and extraction of cadaverine via immobilized cells
  • Apparatus for continuous production and extraction of cadaverine via immobilized cells

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Citing the patented method and steps of immobilizing cells, the immobilized cells are evenly placed in the immobilized cell column 25 of the pentamethylenediamine conversion integrated tank 21 . In order to ensure that the immobilized cells have a larger contact area, the immobilized cell bacteria column 25 is assembled from multi-layer uniform stainless steel wire mesh column boxes. The temperature detection feedback system 23 regulates the power of the heater 61 to ensure that the temperature of the lysine salt solution entering the pentamethylenediamine conversion integrated tank 21 is 37° C., and the pH detection feedback system 22 adjusts the third solenoid valve 32 and the fourth solenoid valve 33 to ensure that each The ph of the lysine salt solution entering the pentamethylenediamine conversion integrated tank 21 for the first time is the same. Pyridoxal phosphate is added to the feed liquid at a ratio of 3:1000 (mmol:ml), and the flow rate of the raw material t...

Embodiment 2

[0048] When the solution containing pentamethylenediamine flows through the calcium phytate column, the calcium phytate column can adsorb the pentamethylenediamine in the solution on the calcium phytate column, reducing the concentration of pentamethylenediamine in the solution and lowering the pH of the solution at the same time. However, the adsorption is not very stable. After experiments, it was found that part of the pentamethylenediamine adsorbed by the calcium phytate column can be eluted with a solution. It can also re-adsorb pentamethylenediamine to achieve the purpose of recycling. First, use the same concentration and volume of pentamethylenediamine conversion solution to flow through the calcium phytate column to absorb pentamethylenediamine, and then use solutions with pH of 4.5, 7.0, and 13.4 to elute the pentamethylenediamine on the calcium phytate column at the same flow rate. Pentylenediamine. The concentration of the solution before and after passing through...

Embodiment 3

[0052] First turn on the three extraction centrifuges in order, and adjust the frequency of the motor to 45hz. After the extraction centrifuges are stable and normal, first pump the heavy phase from the heavy phase inlet at a flow rate of 100ml / min, and wait for the liquid to flow out from the heavy phase outlet. Immediately pump the light phase from the light phase inlet at a flow rate of 100ml / min, wait for 15-20 minutes after the output of the heavy and light phases is stable, start recording the volumes of the heavy and light phases, and re-collect the heavy and light phases from the outlet Liquid and measure the volume. After running for a period of time, turn off the pump at the entrance of the heavy and light two phases at the same time. When there is no liquid flowing out of the heavy and light two phases, turn off the motor of the centrifugal extractor, and finally let off a small amount of liquid in the centrifugal extractor. . Measure the volume of feed liquid (heav...

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Abstract

The invention discloses an apparatus for continuous production and extraction of cadaverine via immobilized cells. The apparatus comprises a feed conveying part, a cadaverine converting part, a pH value adjusting part, a column adsorption and elution part and a centrifugal extraction part, wherein a feed tank, an integrated cadaverine conversion tank and a calcium phytate column are in circulation connection via pipelines; a pH value adjusting liquid tank is connected on a pipeline located between the feed tank and integrated cadaverine conversion tank via a pipeline; an eluate storage tank is connected with the calcium phytate column via a pipeline; and a centrifugal extraction machine is connected with the calcium phytate column. Compared with the prior art, the apparatus for continuous production and extraction of cadaverine via the immobilized cells perfectly overcome problems in continuous production and extraction of cadaverine, is applicable to large-scale production and operation of cadaverine, and has the characteristics of simple operation, high equipment utilization rate, stable product quality, sufficient utilization of raw materials, easiness in promotion, etc.

Description

technical field [0001] The invention relates to a compound production and extraction device, in particular to a device for continuous production and extraction of pentamethylenediamine by immobilized cells, belonging to the technical field of industrial processing and production. Background technique [0002] Pentylenediamine (cadaverine, also known as cadaverine) is a colorless viscous fuming liquid with a special smell. Melting point: 9°C, soluble in water, ethanol, etc. Pentylenediamine monomer is an intermediate with important and extensive industrial uses, widely used in various polyamide products (nylon), chelating agents and additives, etc. With the improvement of environmental awareness and the rise of bio-economy, pentamethylenediamine as a platform compound has attracted more and more attention. The mass-produced pentamethylenediamine by biological method can replace the traditional biogenic amine produced by chemical industry—hexamethylenediamine, and can synthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00C07C209/86C07C211/09
CPCC07C209/86C12M25/00C12M41/12C12M41/26C12M41/32C12M47/10C07C211/09
Inventor 陈可泉何育美马伟超王璟齐雁斌陈西程琪欧阳平凯
Owner NANJING UNIV OF TECH
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