Method for rapidly detecting multiple biogenic amines in dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy

A high-efficiency thin-layer chromatography and surface-enhanced Raman technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to distinguish chromatographic separation results, and achieve the effect of broadening the scope of application

Active Publication Date: 2017-05-24
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because it is difficult to distinguish the interference of adjacent substances in the chromatographi

Method used

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  • Method for rapidly detecting multiple biogenic amines in dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy
  • Method for rapidly detecting multiple biogenic amines in dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy
  • Method for rapidly detecting multiple biogenic amines in dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of nano-silver colloid: take 5 mL of commercially available nano-silver particle suspension (Aladdin Reagent Company) and centrifuge at 4000r / min for 10 min, discard 4.5 mL of supernatant, and place the remaining 0.5 mL of liquid in a mixing chamber Vibrate fully on the device for 1min to resuspend to obtain 10 times concentrated nano-silver colloid;

[0022] (2) Preparation of standard solutions of biogenic amines: use methanol as solvent to prepare standard solutions of tyramine, cadaverine and putrescine with a concentration of 0.05 mg / mL;

[0023] (3) High-efficiency thin-layer chromatographic separation: use a thin-layer chromatography spotting instrument to accurately spot the 2-4 μL standard solution of biogenic amines prepared in step (2). After the spotting is completed, develop with a developing solution, and develop up to 50 mm to take out the silica gel plate; derivatized by dipping with 0.1 mg / mL fluorescamine in acetone solution, removed th...

Embodiment 2

[0027] Weigh 10g of cheese sample, add 10mL of 10% trichloroacetic acid aqueous solution and mix it into a homogenate with a homogenizer. Add 0.1mL 0.25g / mL NaOH solution to the homogenate, shake and mix well, place it in a centrifuge at 3000r / min for 5min, take 2mL supernatant, and filter it with a 0.45 μm fiber membrane to get rid of the main matrix interference cheese samples.

[0028] Using 0.5 MPa nitrogen gas as the carrier, use a 100 μL syringe (CAMAG) to accurately spot 2-4 μL of each cheese sample and biogenic amine standard on a 10×10 cm thin-layer plate with Linomat 5, and the spotted strips 6mm long, the strips are 8mm from the bottom, 12mm from the left end, and the strips are spaced 1.7mm apart. After sample pointing, use the ADC-2 (CAMAG) developing instrument to develop. Before developing, inject 10 mL of mobile phase into another tank to make the cylinder reach a saturated state. Take 10 mL of the optimized developing solution (methanol / acetone / ammonia water...

Embodiment 3

[0032] Weigh 10g of yogurt, add 10mL of 10% trichloroacetic acid aqueous solution and mix it into a homogenate with a homogenizer. Add 0.1mL of 0.25g / mL NaOH solution to the homogenate, shake and mix well, place it in a centrifuge at 3000r / min for 5min, take 2mL of the supernatant, and filter it with a 0.45 μm fiber membrane to get rid of the main matrix interference yogurt samples.

[0033]Using 0.5 MPa nitrogen gas as the carrier, use a 100 μL syringe (CAMAG) to accurately spot 2-4 μL of each yogurt sample and biogenic amine standard on a 10×10 cm thin-layer plate with Linomat 5. The strip length is 6mm, the strip is 8mm from the bottom, 12mm from the left end, and the strip spacing is 1.7mm. After sample pointing, use the ADC-2 (CAMAG) developing instrument to develop. Before developing, inject 10 mL of mobile phase into another tank to make the cylinder reach a saturated state. Take 10 mL of developing solution (methanol / acetone / ammonia water (25%)=3 / 7 / 0.5 (v / v)), develo...

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Abstract

The invention provides a method for rapidly detecting multiple biogenic amines in a dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy, and belongs to the technical field of food detection. The method comprises the steps of preparation of nano silver colloid, preparation of samples, separation based on high performance thin layer chromatography, HPTLC-SERS detection and the like. The HPTLC-SERS detection method for identifying structures of the multiple biogenic amines (tyramine, putrescine and cadaverine) is established, and has the advantages of economical efficiency, rapidness, simplicity and convenience; furthermore, by virtue of the selectivity enhancement function of a new synergist, the application range of HPTLC-SERS detection is expanded, and a new visual field is opened for directly and rapidly detecting a chromatoplate for chemical derivatization.

Description

technical field [0001] The invention relates to a method for rapid detection of multiple biogenic amines in dairy products by combining high-efficiency thin-layer chromatography and adjustable surface-enhanced Raman spectroscopy, in particular to a fluorescently labeled biogenic amine molecule and a synergist used to suppress fluorescence derivative reagents The masking effect caused by the invention belongs to the technical field of food detection. Background technique [0002] Biogenic amines are the products of amino acid decarboxylation and are an important indicator for evaluating the degree of food spoilage. Foods containing high levels of biogenic amines pose a serious risk to consumers and their potential toxicity can cause consumer allergies. Therefore, developing a method for rapid detection of biogenic amine residues in food has become an urgent problem to be solved in the supervision of the toxicity hazards of biogenic amines in the food industry. [0003] In t...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 陈益胜王杨徐学明杨哪吴凤凤
Owner JIANGNAN UNIV
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