39 results about "Tetranychus urticae" patented technology

The invention belongs to the technical field of biological prevention and control of plant pests, and relates to application of streptomyces sdu1201 and / or secondary metabolites in prevention and control of plant pests. Research finds that the streptomyces sp. Sdu1201 and the secondary metabolite thereof have the effect of preventing and treating tetranychus urticae and root-knot nematode.

The invention relates to preparation and application of a novel trifluoroalkyl sulfur-containing phenyl heterocyclic ring derivative. Specifically, the invention relates to a compound as shown in formula (I) containing a trifluoroalkyl thiophenyl heterocyclic derivative, an isotope labeled compound of the compound, an optical isomer, a geometric isomer, a tautomer or an isomer mixture of the compound, or a pharmaceutically acceptable salt of the compound, and application of the compound in preparation of insecticides for pest control. The compound can achieve a better pest control effect at a lower dosage, especially for pests and pest mites such as tetranychus cinnabarinus, tetranychus urticae and panonychus citri.

The invention relates to the field of crop pest monitoring, in particular to a method for identifying or monitoring tetranychid damage based on hyperspectrum. In order to solve the problems that the tetranychus urticae is small in size, the harmful symptoms are not easy to discover, and detection and monitoring are difficult, the method has the advantages of a traditional imaging technology and a spectrum technology based on the characteristic that a hyperspectral imaging technology has an image-spectrum unification function, hyperspectral information of plant leaves damaged by the tetranychus urticae is obtained by utilizing a hyperspectral imager, and the method is used for detecting and monitoring the tetranychus urticae. The rapid monitoring and identification method is developed by carrying out classification research on the hyperspectral reflectivity data of the plants, and optional technical support and scientific guarantee are provided for agricultural production.

The invention discloses an agricultural synergist containing astragalus polysaccharide and application of the agricultural synergist, and belongs to the technical field of killing agents for biopesticides. The synergist comprises astragalus polysaccharide, a polysorbate / lecithin compound emulsifier, a carboxymethyl chitosan / siloxane penetrant and a modified accelerant synthesized by palladium catalysis. Polysaccharide is structurally modified through a blue light LED induced tetra (triphenylphosphine) palladium catalytic reaction, and the epidermal penetration efficiency of the biopesticide on tetranychus urticae is improved by 3.2 times in combination with the synergistic permeation effect of a siloxane derivative. The mite killing rate of the suspending agent prepared according to a preferable formula reaches 92.5% within 24 hours, and the lasting period is prolonged to 14 days. The system overcomes the defects of slow effectiveness and poor stability of the traditional biopesticide, by means of a targeted combination mechanism of natural components, the environment friendliness is kept while the original pesticide dosage is reduced by 50%, and the system is particularly suitable for the pest control requirement of organic agriculture.

The invention relates to the technical field of traditional Chinese medicinal material production, in particular to a green prevention and control method for main leaf pests of tetranychus urticae and tetranychus crataegi in the under-forest ecological planting process of polygonatum sibiricum, and the method comprises the steps of S1, egg overwintering prevention and control, S2, non-host plant repelling, S3, biological agent killing, S4, agronomic measure blocking, and S5, late autumn tannin insect prevention and egg inhibition. Aiming at the technical problem of prevention and control of leaf mites of polygonatum sibiricum, from the perspective of sustainable development of traditional Chinese medicine ecological agriculture, an ecological technology is used in comprehensive treatment of leaf pests of polygonatum sibiricum, a green prevention and control implementation path for tetranychus urticae and tetranychus crataegus is provided, pollution of agricultural chemicals can be reduced, and the environment-friendly prevention and control of leaf mites of polygonatum sibiricum is realized. Meanwhile, green forest ecological traditional Chinese medicine products are produced, the rhizome yield per unit area is increased by 9.26%, it is ensured that the first-grade product rate is higher than that of conventional cultivation by 13.58% or above, and economic benefits are improved.

The invention discloses dsRNA / fused dsRNA and application of the dsRNA / fused dsRNA in prevention and treatment of spider mites, the dsRNA is dsRpt3, and the fused dsRNA is obtained by fusing dsRNA of a plurality of genes of the same gene family of targeted spider mites; the gene family is selected from a proteasome ATP enzyme family and an apolipoprotein family; the nucleotide sequence of one chain of the fused dsRNA is as shown in any one of SEQ ID NO.1-4. The invention also discloses a preparation method of the fused dsRNA. Compared with the existing dsRNA for preventing and treating a single variety of spider mites, the dsRNA / fused dsRNA can prevent and treat various spider mites at the same time, is expected to be applied to preparation of biopesticides for preventing and treating spider mites, reduces the use of chemical pesticides, protects the environment, and reduces the damage of the chemical pesticides.

The invention discloses an Srp54k target gene for preventing and treating tetranychus crataegus and tetranychus urticae and application of the Srp54k target gene, dsRNA synthesized by the Srp54k target gene and application of the dsRNA. The nucleotide sequences of the Srp54k target gene are shown as SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide sequences of the dsRNA are shown as SEQ ID NO.3 and SEQ ID NO.4; after SRP54K is interfered, eggs of tetranychus crataegi and tetranychus urticae are caused, the survival rate is obviously influenced, and the prevention and treatment aim is achieved; the method is convenient to operate, good in effectiveness and sensitivity and high in insecticidal efficiency, has the advantages of being environmentally friendly and the like, and has a good application prospect.

The present invention relates to the preparation and use of novel trifluoroalkylthiophenylpyridine derivatives. Specifically, the present invention relates to compounds of formula (I-1) or formula (I-2) as trifluoroalkylthiophenylpyridine derivatives, or isotope labeled compounds thereof, or optical isomers, geometric isomers, tautomers or mixtures of isomers thereof, or agriculturally acceptable salts thereof, as well as the use of said compounds in the preparation of insecticides for pest control. These compounds are capable of achieving better pest control effects at lower dosages, especially against pests and mites such as Tetranychus cinnabarinus, Tetranychus urticae, and Panonychus citri.

The present invention discloses a gene mutation site of field mite resistance to abamectin and its application, belonging to the technical field of field resistance detection. The present invention uses the cytochrome P450 detoxifying enzyme CYP392A11 gene as a resistance detection marker for field mites to abamectin, and provides a primer pair for amplifying the CYP392A11 gene to verify whether there is a point mutation, thereby completing the rapid identification of field mite resistance to abamectin. The present invention detects the resistance level of field mites to abamectin through the single-base site mutation in the promoter region of CYP392A11 of field mites, and can quickly detect the resistance level of Tetranychus urticae to abamectin in the field, thereby providing technical support for the rational use of pesticides.

The invention belongs to the technical field of gene detection, and particularly relates to a tetranychus urticae cytochrome P450 gene and application thereof to detection and / or monitoring of avermectin resistance of tetranychus urticae. The CYP392A13 gene shows up-regulated expression in a tetranychus urticae resistant population, and the correlation between the target gene and the avermectin resistance of the tetranychus urticae is further confirmed from the back side and the front side through RNAi and in-vitro metabolic activity tests. Therefore, the specific primer pair, the primer group, the kit and the method for detecting the abamectin resistance of the tetranychus urticae are researched and developed, if the expression quantity of a target gene in a population to be detected is obviously higher than that of a tetranychus urticae sensitive population, the population to be detected has the abamectin resistance; a key molecular tool is provided for establishing an abamectin resistance early warning system, and a drug resistance treatment strategy can be formulated in time.

The invention discloses an eIF4A-1 target gene for preventing and treating tetranychus crataegi and tetranychus urticae, dsRNA synthesized by the eIF4A-1 target gene and application of the dsRNA. The nucleotide sequence of the eIF4A-1 target gene is as shown in SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO.3 and SEQ ID NO.4; the dsRNA solution with the target gene being EIF4A-1 is used for soaking eggs of tetranychus crataegi and tetranychus urticae to influence the survival rate, and the prevention and treatment purpose is achieved; the method is convenient to operate, good in effectiveness and sensitivity and high in insecticidal efficiency, has the advantages of being environmentally friendly and the like, and has a good application prospect.

The invention belongs to the technical field of pesticides, and relates to a pesticide composition and application thereof. The pesticide composition comprises an active component A and an active component B, the active component A is a compound shown in a formula (I), and the active component B is one or more selected from acaricides. The pesticide composition can effectively prevent and control multiple phytophagous pest mites, especially two-spotted spider mites, has a synergistic effect in a certain proportion range, reduces the use cost, reduces the dosage, prolongs the lasting period and delays the development of drug resistance.

The application discloses a method for green prevention and control of Tetranychus urticae by Neoseiulus barkeri mediated greenhouse 'natural enemy + crop + habitat regulation' integrated method, and belongs to the technical field of green prevention and control of greenhouse pests. The method takes N. barkeri as a core control factor, and organically integrates precise release of natural enemies, healthy cultivation of crops and dynamic regulation of greenhouse habitats. Specifically, the method comprises the following steps: installing a disinfection and pest prevention net before planting; cultivating crops in a healthy manner, and planting driving-avoiding and covering plants in intercropping; releasing 100-300 N. barkeri per square meter, and then releasing the N. barkeri once every 1-2 weeks; planting nectar plants, regulating temperature and humidity to 22-28 DEG C and 60%-75%, and hanging yellow and blue plates; regularly monitoring, and releasing natural enemies or selecting safe biological pesticides for auxiliary prevention and control when the control threshold is exceeded; and cleaning and disinfecting after pulling seedlings and rotating crops. The method realizes persistent colonization and population self-maintenance of natural enemies, significantly reduces the use of chemical pesticides, and improves crop yield and quality.

The invention relates to the technical field of RNA (Ribonucleic Acid) interference biopesticides, in particular to dsRNA for reducing the abamectin resistance level by targeting a gene CCE59 and application of the dsRNA. The dsRNA molecule is composed of a positive-sense strand and a complementary antisense strand, and the nucleotide sequence of the positive-sense strand is shown as SEQ ID NO.1. The dsRNA molecule further comprises any one of the following applications: (1) the dsRNA molecule is used for preventing and treating tetranychus urticae; (2) the composition is used for reducing the resistance of tetranychus urticae to abamectin; and (3) preparing a product for preventing the tetranychus urticae and / or reducing the resistance of the tetranychus urticae to abamectin. The invention mainly solves the problem that the drug resistance of pests is enhanced in the process of preventing and treating tetranychus urticae by using the traditional chemical pesticide. The design and synthesis of dsRNA are carried out, the silencing effect of the dsRNA on CCE59 is realized, the resistance level to abamectin is reduced, and the death of tetranychus urticae is promoted, so that the purpose of treating the tetranychus urticae is achieved.

The invention discloses a KASP labeled primer for detecting the resistance of tetranychus urticae to carbamate insecticides and application of the KASP labeled primer, and relates to the technical field of agricultural biology. The KASP labeled primer group disclosed by the invention comprises a primer combination which is used for detecting the resistance mutations of TurtG119S, TurtF331W, TurtF331Y, TurtA201S, TurtT280A and TurtG328A of the carbamate insecticide. The KASP labeled primer group disclosed by the invention comprises a primer combination which is used for detecting the resistance mutations of TurtG119S, TurtF331W, TurtF331Y, TurtA201S, TurtT280A and TurtG328A. The group of KASP labeled primers has good specificity and high sensitivity, and can accurately detect the resistance mutation frequency of the tetranychus urticae to carbamate insecticides.

The invention belongs to the technical field of microbial pesticides, and discloses a bacillus thuringiensis ZYLT064 with efficient insecticidal activity on cotton aphid and tetranychus urticae and application of the bacillus thuringiensis ZYLT064. The preservation number of the strain in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20242335; the strain can generate insecticidal active substances of bacillus thuringiensis and trans-aconitic acid, fermented supernate of the strain shows remarkable insecticidal activity on cotton aphids and tetranychus urticae, the fatality rate of the cotton aphids biologically tested by feeding in a laboratory reaches 87%, the fatality rate of the tetranychus urticae is 80.9%, and the fatality rate of the tetranychus urticae biologically tested by potting in a greenhouse reaches 72.5%. Therefore, the strain disclosed by the invention has the potential of being developed into a multifunctional biological control product and has a wide application prospect.

The invention discloses a tetranychus urticae classification identification and automatic counting system and method based on deep learning, and relates to the technical field of agricultural pest monitoring. By integrating the optical microscope, the high-precision image acquisition equipment and the deep learning model, automatic detection and counting functions of mites are realized, and the technical problems of low efficiency, high subjectivity, poor accuracy and the like in a traditional manual detection method are solved; by adopting an improved YOLO series algorithm and a multi-scale feature fusion technology, the system can identify multiple development stages (including eggs, larvae, nymphs and adult mites) and predatory mites of tetranychus urticae at the same time, and the detection accuracy and efficiency are greatly improved.

The invention discloses a 3-substituted-4-pyridyl-1, 2, 5-thiadiazole compound with a novel structure or a salt of the 3-substituted-4-pyridyl-1, 2, 5-thiadiazole compound. The structural general formula of the compound is shown as a formula (I). The compound disclosed by the invention has an excellent prevention and treatment effect on agricultural pest mites, particularly tetranychidae (such as tetranychus cinnabarinus and tetranychus urticae), acaridae and the like, shows higher biological activity and better selectivity compared with known analogues containing benzene rings, and has the advantages of simple and convenient preparation method and good environmental compatibility. The invention also comprises a preparation method of the compound, an agricultural composition containing the compound and application of the compound to prevention and control of pest mites.

Contact insecticide and acaricide suitable for organic agriculture for the management of mites and soft-bodied insects, and its method of production. This invention relates to the creation of a formulation applicable in both organic and conventional agriculture for the protection of plants against mites and soft-bodied insect pests of crops. The action of the formulation is due to the inseparable combination of the active substance, which is the potassium salts of fatty acids, with mixtures of mono- and diglycerides of fatty acids as well as vegetable oils that increase the solubility of the formulation in the spray liquid and its effectiveness. The acaricidal and insecticidal action of the final formulation is further enhanced by the addition of acyclic sesquiterpenes and caraway essential oil, which cause intense mobility of the mites / insects, thereby increasing the contact time of the pests with the active ingredient, leading to their immediate death. This invention represents an advancement in current technology by proposing a combination of active ingredients that act synergistically, ensuring the effectiveness of the formulation even at low concentrations of the active substance. The low concentration of the active ingredient, combined with the use of natural and environmentally friendly components, results in the proposed formulation with an improved toxicological and environmental profile and minimizes the risk of phytotoxicity often observed from the use of plant protection products with high concentrations of potassium salts of fatty acids. This patent also describes: a) the method of production of the active ingredient and the final formulation (additional examples of proposed compositions are provided in the supplementary section), b) the proposed method of application of the formulation in the field, c) laboratory studies conducted on the effectiveness of the proposed formulation against the agricultural pest Tetranychus urticae (two-spotted spider mite) and the study of its compatibility with the beneficial predator Phytoseiulus persimilis.

The invention belongs to the technical field of biological prevention and control, and particularly relates to an application of a Tilletia foetida Kuhn bacterial suspension in prevention and control of tetranychus urticae Koch. The Tilletia foetida Kuhn bacterial suspension can be applied to prevention and control of tetranychus urticae Koch, and the Tilletia foetida Kuhn bacterial suspension can be applied to prevention and control of tetranychus urticae Koch. The Tilletia foetida suspension can be used for directly or indirectly preventing and treating tetranychus urticae.

The invention relates to preparation and application of a novel trifluoroalkyl-containing thiophenyl pyridine derivative. Specifically, the invention relates to a compound as shown in a formula (I-1) or (I-2) or an isotope labeled compound of the compound, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture of the compound, or a pharmaceutically acceptable salt of the compound, the invention also relates to application of the compound in preparation of insecticide for pest control. The compound can achieve a better pest control effect at a lower dosage, especially for pests and pest mites such as tetranychus cinnabarinus, tetranychus urticae and panonychus citri.

The invention relates to the technical field of RNA interference biopesticides, in particular to a target gene and dsRNA for preventing and treating tetranychus urticae and application of the target gene and dsRNA. The target gene is selected from at least one of nucleotide sequences of SEQ ID NO.1 to SEQ ID NO.7; the dsRNA molecule is composed of a positive-sense strand and a complementary antisense strand, wherein the positive-sense strand is selected from at least one of nucleotide sequences of SEQ ID NO.8-SEQ ID NO.14. The dsRNA molecule has a nucleotide sequence of SEQ ID NO.12-SEQ ID NO.14. Some gene loci having important influence on the growth and development of the tetranychus urticae in the genome of the tetranychus urticae serve as RNAi target genes, dsRNA molecules are designed for each target gene, and the dsRNA molecules can achieve efficient prevention and control of the tetranychus urticae, are good in sensitivity and high in insecticidal efficiency, have the advantages of being environmentally friendly and the like, and have good application prospects in prevention and control of the tetranychus urticae and control of the tetranychus urticae and control of the tetranychus urticae. Good application prospects are realized.

The invention belongs to the technical field of gene detection, and particularly relates to a tetranychus urticae cytochrome P450 gene and application thereof to detection and / or monitoring of avermectin resistance of tetranychus urticae. The CYP392A13 gene shows up-regulated expression in a tetranychus urticae resistant population, and the correlation between the target gene and the avermectin resistance of the tetranychus urticae is further confirmed from the back side and the front side through RNAi and in-vitro metabolic activity tests. Therefore, the specific primer pair, the primer group, the kit and the method for detecting the abamectin resistance of the tetranychus urticae are researched and developed, if the expression quantity of a target gene in a population to be detected is obviously higher than that of a tetranychus urticae sensitive population, the population to be detected has the abamectin resistance; a key molecular tool is provided for establishing an abamectin resistance early warning system, and a drug resistance treatment strategy can be formulated in time.

The invention discloses an agricultural synergist containing cinnamyl aldehyde and application of the agricultural synergist, and belongs to the technical field of killing agents for biopesticides. The synergist is prepared from the following components in parts by mass: 20 to 45 parts of cinnamyl aldehyde, 5 to 15 parts of silver nanoparticles, 2 to 8 parts of a surfactant, 20 to 40 parts of a gamma-cyclodextrin metal organic framework carrier, 5 to 15 parts of methyl silicone oil, 2 to 3 parts of polydimethylsiloxane, 5 to 10 parts of polyvinyl alcohol and 120 to 170 parts of deionized water. The synergist is applied to preparation of insecticides, the dosage form is a suspending agent and the like, and the synergist is used for killing tetranychus urticae. Through cooperation of multiple components, the utilization rate of cinnamyl aldehyde is increased by more than 60%, the insecticidal activity is increased by 30% or above, the stability is high, residues are low, and green agriculture is promoted.

The invention relates to the technical field of etoxazole resistance monitoring, in particular to application of a resistance gene haplotype CHS1-mut2 to accurate monitoring of etoxazole resistance. The nucleotide sequence table of the haplotype CHS1-mut2 is as shown in SEQ ID No 1. According to the application of the resistance gene haplotype CHS1-mut2 in accurately monitoring the etoxazole resistance provided by the invention, whether etoxazole has a good mite killing effect when facing tetranychus urticae or not can be detected by detecting the resistance mutation frequency of a medicament to etoxazole.

The invention discloses dsRNA (double-stranded ribonucleic acid) targeting a tetranychus urticae bunchy protein gene CS640 and application of the dsRNA. The dsRNA consists of a nucleotide sequence as shown in SEQ ID No.6 and a reverse complementary sequence of the nucleotide sequence as shown in SEQ ID No.6. Specific dsRNA is synthesized and delivered into tetranychus urticae eggs by a drop method, so that the development process of the tetranychus urticae can be effectively blocked, and a remarkable lethal effect is caused; and the synthesized specific dsRNA is harmless to the natural enemy organism neoseiulus barkeri, and has good safety. Therefore, the gene has important application value as a mite RNAi prevention and control target.

The invention provides cladosporium cladosporioides HKLJ-2 and application of the cladosporium cladosporioides HKLJ-2 in prevention and control of tetranychus urticae, and relates to the technical field of biocontrol bacteria. The cladosporium cladosporioides HKLJ-2 strain is separated from a tetranychus urticae body dead on an angelica sinensis plant, is identified as cladosporium cladosporioides, is named as cladosporium cladosporioides HKLJ-2, is preserved in the China Center for Type Culture Collection (Wuhan University) on September 10, 2024, has the preservation number of CCTCC NO: M 20241952, and has the preservation number of CCTCC NO: M 20241952. The spore liquid has strong pathogenicity to tetranychus urticae adults in a normal temperature environment, is expected to be developed into biocontrol bacteria of tetranychus urticae, and provides more choices for biological control of tetranychus urticae.

The invention belongs to the technical field of agricultural biology, and particularly relates to a nucleic acid pesticide for preventing and controlling tetranychus urticae and application of the nucleic acid pesticide. Research finds that after the expression quantity of the Pb1 gene of the tetranychus urticae is reduced by an RNA interference technology, the growth and development abnormity and the death rate of the tetranychus urticae are obviously improved, the medial lethal concentration of the tetranychus urticae is 4.86 ng / mu L, and the prevention and control effects on eggs, larvae and adults are 92.76%, 84.24% and 96.14% respectively when the double-stranded RNA of Pb1 is sprayed on the surfaces of plants, so that the application of the Pb1 gene of the tetranychus urticae is promoted, and the application of the Pb1 gene of the tetranychus urticae is promoted. Therefore, the target gene has great potential in the prevention and control of the tetranychus urticae, and meanwhile, a new way is provided for the prevention and control of the tetranychus urticae by utilizing the double-stranded RNA.

The invention relates to the technical field of gene editing, in particular to a gRNA composition of a targeted CYP392E1 gene for preventing and treating tetranychus urticae and application of the gRNA composition. The CYP392E1 gene of which the nucleotide sequence is shown as SEQ ID NO.1 and any one of the following applications of gRNA of which the nucleotide sequence is shown as SEQ ID NO.2: 1) preventing and treating tetranychus urticae, and / or preparing a medicine for preventing and treating tetranychus urticae; and 2) reducing the resistance of the tetranychus urticae to the acaricide, and / or preparing a drug for reducing the resistance of the tetranychus urticae to the acaricide. The gRNA is designed by applying a gene knockout technology, especially aiming at a detoxification enzyme gene for regulating detoxification metabolism in a pest mite body, a compound consisting of the gRNA composition and cas9 protein is delivered into a tetranychus urticae body by utilizing a microinjection technology, and the gRNA composition loses the function of a target gene, reduces the detoxification metabolism capability of a medicament, and can be used for treating the pest mite body. The resistance reversion of the two-spotted spider mites is promoted, so that the aim of preventing and treating the two-spotted spider mites is

The application belongs to the technical field of biological control of plant diseases and insect pests, and relates to application of streptomyces sdu1201 and / or secondary metabolites thereof in control of plant pests. The application finds that the streptomyces sdu1201 and the secondary metabolites thereof have the effects of controlling the two-spotted spider mite and the root-knot nematode.