18 results about "Tetranychus urticae" patented technology

The present invention relates to the preparation and use of novel trifluoroalkylthiophenylpyridine derivatives. Specifically, the present invention relates to compounds of formula (I-1) or formula (I-2) as trifluoroalkylthiophenylpyridine derivatives, or isotope labeled compounds thereof, or optical isomers, geometric isomers, tautomers or mixtures of isomers thereof, or agriculturally acceptable salts thereof, as well as the use of said compounds in the preparation of insecticides for pest control. These compounds are capable of achieving better pest control effects at lower dosages, especially against pests and mites such as Tetranychus cinnabarinus, Tetranychus urticae, and Panonychus citri.

The invention belongs to the technical field of gene detection, and particularly relates to a tetranychus urticae cytochrome P450 gene and application thereof to detection and / or monitoring of avermectin resistance of tetranychus urticae. The CYP392A13 gene shows up-regulated expression in a tetranychus urticae resistant population, and the correlation between the target gene and the avermectin resistance of the tetranychus urticae is further confirmed from the back side and the front side through RNAi and in-vitro metabolic activity tests. Therefore, the specific primer pair, the primer group, the kit and the method for detecting the abamectin resistance of the tetranychus urticae are researched and developed, if the expression quantity of a target gene in a population to be detected is obviously higher than that of a tetranychus urticae sensitive population, the population to be detected has the abamectin resistance; a key molecular tool is provided for establishing an abamectin resistance early warning system, and a drug resistance treatment strategy can be formulated in time.

The invention belongs to the technical field of pesticides, and relates to a pesticide composition and application thereof. The pesticide composition comprises an active component A and an active component B, the active component A is a compound shown in a formula (I), and the active component B is one or more selected from acaricides. The pesticide composition can effectively prevent and control multiple phytophagous pest mites, especially two-spotted spider mites, has a synergistic effect in a certain proportion range, reduces the use cost, reduces the dosage, prolongs the lasting period and delays the development of drug resistance.

The application discloses a method for green prevention and control of Tetranychus urticae by Neoseiulus barkeri mediated greenhouse 'natural enemy + crop + habitat regulation' integrated method, and belongs to the technical field of green prevention and control of greenhouse pests. The method takes N. barkeri as a core control factor, and organically integrates precise release of natural enemies, healthy cultivation of crops and dynamic regulation of greenhouse habitats. Specifically, the method comprises the following steps: installing a disinfection and pest prevention net before planting; cultivating crops in a healthy manner, and planting driving-avoiding and covering plants in intercropping; releasing 100-300 N. barkeri per square meter, and then releasing the N. barkeri once every 1-2 weeks; planting nectar plants, regulating temperature and humidity to 22-28 DEG C and 60%-75%, and hanging yellow and blue plates; regularly monitoring, and releasing natural enemies or selecting safe biological pesticides for auxiliary prevention and control when the control threshold is exceeded; and cleaning and disinfecting after pulling seedlings and rotating crops. The method realizes persistent colonization and population self-maintenance of natural enemies, significantly reduces the use of chemical pesticides, and improves crop yield and quality.

The invention relates to the technical field of RNA (Ribonucleic Acid) interference biopesticides, in particular to dsRNA for reducing the abamectin resistance level by targeting a gene CCE59 and application of the dsRNA. The dsRNA molecule is composed of a positive-sense strand and a complementary antisense strand, and the nucleotide sequence of the positive-sense strand is shown as SEQ ID NO.1. The dsRNA molecule further comprises any one of the following applications: (1) the dsRNA molecule is used for preventing and treating tetranychus urticae; (2) the composition is used for reducing the resistance of tetranychus urticae to abamectin; and (3) preparing a product for preventing the tetranychus urticae and / or reducing the resistance of the tetranychus urticae to abamectin. The invention mainly solves the problem that the drug resistance of pests is enhanced in the process of preventing and treating tetranychus urticae by using the traditional chemical pesticide. The design and synthesis of dsRNA are carried out, the silencing effect of the dsRNA on CCE59 is realized, the resistance level to abamectin is reduced, and the death of tetranychus urticae is promoted, so that the purpose of treating the tetranychus urticae is achieved.

The invention discloses a tetranychus urticae classification identification and automatic counting system and method based on deep learning, and relates to the technical field of agricultural pest monitoring. By integrating the optical microscope, the high-precision image acquisition equipment and the deep learning model, automatic detection and counting functions of mites are realized, and the technical problems of low efficiency, high subjectivity, poor accuracy and the like in a traditional manual detection method are solved; by adopting an improved YOLO series algorithm and a multi-scale feature fusion technology, the system can identify multiple development stages (including eggs, larvae, nymphs and adult mites) and predatory mites of tetranychus urticae at the same time, and the detection accuracy and efficiency are greatly improved.

The invention discloses a 3-substituted-4-pyridyl-1, 2, 5-thiadiazole compound with a novel structure or a salt of the 3-substituted-4-pyridyl-1, 2, 5-thiadiazole compound. The structural general formula of the compound is shown as a formula (I). The compound disclosed by the invention has an excellent prevention and treatment effect on agricultural pest mites, particularly tetranychidae (such as tetranychus cinnabarinus and tetranychus urticae), acaridae and the like, shows higher biological activity and better selectivity compared with known analogues containing benzene rings, and has the advantages of simple and convenient preparation method and good environmental compatibility. The invention also comprises a preparation method of the compound, an agricultural composition containing the compound and application of the compound to prevention and control of pest mites.

The invention relates to preparation and application of a novel trifluoroalkyl-containing thiophenyl pyridine derivative. Specifically, the invention relates to a compound as shown in a formula (I-1) or (I-2) or an isotope labeled compound of the compound, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture of the compound, or a pharmaceutically acceptable salt of the compound, the invention also relates to application of the compound in preparation of insecticide for pest control. The compound can achieve a better pest control effect at a lower dosage, especially for pests and pest mites such as tetranychus cinnabarinus, tetranychus urticae and panonychus citri.

The invention relates to the technical field of RNA interference biopesticides, in particular to a target gene and dsRNA for preventing and treating tetranychus urticae and application of the target gene and dsRNA. The target gene is selected from at least one of nucleotide sequences of SEQ ID NO.1 to SEQ ID NO.7; the dsRNA molecule is composed of a positive-sense strand and a complementary antisense strand, wherein the positive-sense strand is selected from at least one of nucleotide sequences of SEQ ID NO.8-SEQ ID NO.14. The dsRNA molecule has a nucleotide sequence of SEQ ID NO.12-SEQ ID NO.14. Some gene loci having important influence on the growth and development of the tetranychus urticae in the genome of the tetranychus urticae serve as RNAi target genes, dsRNA molecules are designed for each target gene, and the dsRNA molecules can achieve efficient prevention and control of the tetranychus urticae, are good in sensitivity and high in insecticidal efficiency, have the advantages of being environmentally friendly and the like, and have good application prospects in prevention and control of the tetranychus urticae and control of the tetranychus urticae and control of the tetranychus urticae. Good application prospects are realized.

The invention belongs to the technical field of gene detection, and particularly relates to a tetranychus urticae cytochrome P450 gene and application thereof to detection and / or monitoring of avermectin resistance of tetranychus urticae. The CYP392A13 gene shows up-regulated expression in a tetranychus urticae resistant population, and the correlation between the target gene and the avermectin resistance of the tetranychus urticae is further confirmed from the back side and the front side through RNAi and in-vitro metabolic activity tests. Therefore, the specific primer pair, the primer group, the kit and the method for detecting the abamectin resistance of the tetranychus urticae are researched and developed, if the expression quantity of a target gene in a population to be detected is obviously higher than that of a tetranychus urticae sensitive population, the population to be detected has the abamectin resistance; a key molecular tool is provided for establishing an abamectin resistance early warning system, and a drug resistance treatment strategy can be formulated in time.

The invention discloses an agricultural synergist containing cinnamyl aldehyde and application of the agricultural synergist, and belongs to the technical field of killing agents for biopesticides. The synergist is prepared from the following components in parts by mass: 20 to 45 parts of cinnamyl aldehyde, 5 to 15 parts of silver nanoparticles, 2 to 8 parts of a surfactant, 20 to 40 parts of a gamma-cyclodextrin metal organic framework carrier, 5 to 15 parts of methyl silicone oil, 2 to 3 parts of polydimethylsiloxane, 5 to 10 parts of polyvinyl alcohol and 120 to 170 parts of deionized water. The synergist is applied to preparation of insecticides, the dosage form is a suspending agent and the like, and the synergist is used for killing tetranychus urticae. Through cooperation of multiple components, the utilization rate of cinnamyl aldehyde is increased by more than 60%, the insecticidal activity is increased by 30% or above, the stability is high, residues are low, and green agriculture is promoted.

The invention relates to the technical field of etoxazole resistance monitoring, in particular to application of a resistance gene haplotype CHS1-mut2 to accurate monitoring of etoxazole resistance. The nucleotide sequence table of the haplotype CHS1-mut2 is as shown in SEQ ID No 1. According to the application of the resistance gene haplotype CHS1-mut2 in accurately monitoring the etoxazole resistance provided by the invention, whether etoxazole has a good mite killing effect when facing tetranychus urticae or not can be detected by detecting the resistance mutation frequency of a medicament to etoxazole.

The invention discloses dsRNA (double-stranded ribonucleic acid) targeting a tetranychus urticae bunchy protein gene CS640 and application of the dsRNA. The dsRNA consists of a nucleotide sequence as shown in SEQ ID No.6 and a reverse complementary sequence of the nucleotide sequence as shown in SEQ ID No.6. Specific dsRNA is synthesized and delivered into tetranychus urticae eggs by a drop method, so that the development process of the tetranychus urticae can be effectively blocked, and a remarkable lethal effect is caused; and the synthesized specific dsRNA is harmless to the natural enemy organism neoseiulus barkeri, and has good safety. Therefore, the gene has important application value as a mite RNAi prevention and control target.

The invention provides cladosporium cladosporioides HKLJ-2 and application of the cladosporium cladosporioides HKLJ-2 in prevention and control of tetranychus urticae, and relates to the technical field of biocontrol bacteria. The cladosporium cladosporioides HKLJ-2 strain is separated from a tetranychus urticae body dead on an angelica sinensis plant, is identified as cladosporium cladosporioides, is named as cladosporium cladosporioides HKLJ-2, is preserved in the China Center for Type Culture Collection (Wuhan University) on September 10, 2024, has the preservation number of CCTCC NO: M 20241952, and has the preservation number of CCTCC NO: M 20241952. The spore liquid has strong pathogenicity to tetranychus urticae adults in a normal temperature environment, is expected to be developed into biocontrol bacteria of tetranychus urticae, and provides more choices for biological control of tetranychus urticae.

The invention relates to the technical field of gene editing, in particular to a gRNA composition of a targeted CYP392E1 gene for preventing and treating tetranychus urticae and application of the gRNA composition. The CYP392E1 gene of which the nucleotide sequence is shown as SEQ ID NO.1 and any one of the following applications of gRNA of which the nucleotide sequence is shown as SEQ ID NO.2: 1) preventing and treating tetranychus urticae, and / or preparing a medicine for preventing and treating tetranychus urticae; and 2) reducing the resistance of the tetranychus urticae to the acaricide, and / or preparing a drug for reducing the resistance of the tetranychus urticae to the acaricide. The gRNA is designed by applying a gene knockout technology, especially aiming at a detoxification enzyme gene for regulating detoxification metabolism in a pest mite body, a compound consisting of the gRNA composition and cas9 protein is delivered into a tetranychus urticae body by utilizing a microinjection technology, and the gRNA composition loses the function of a target gene, reduces the detoxification metabolism capability of a medicament, and can be used for treating the pest mite body. The resistance reversion of the two-spotted spider mites is promoted, so that the aim of preventing and treating the two-spotted spider mites is

The application belongs to the technical field of biological control of plant diseases and insect pests, and relates to application of streptomyces sdu1201 and / or secondary metabolites thereof in control of plant pests. The application finds that the streptomyces sdu1201 and the secondary metabolites thereof have the effects of controlling the two-spotted spider mite and the root-knot nematode.

The application belongs to the technical field of molecular biology, and particularly relates to application of reducing SlASR3 gene expression in optimization of tomato traits. The technical problem to be solved by the application is to provide more technical means for improvement and promotion of tomato traits. The technical scheme of the application is application of a substance for reducing SlASR3 gene expression in increasing plant cuticular hair, plant avoidance to Tetranychus urticae Koch or improvement of plant fruit resistance to cold damage stress. The application obtains a gene editing strain of knocking out tomato SlASR3 through gene editing technology, the number of VI type cuticular hair of the leaf of the strain is greatly increased, and the resistance of the tomato plant to Tetranychus urticae Koch is enhanced; meanwhile, the tolerance of the tomato fruit to cold damage is greatly improved.

The present application relates to trifluoroethyl sulfide derivatives and its preparation method and application. It is synthesized by sulfurylation, reduction, nucleophilic substitution and other reactions with 4-chloro-2-methyl aniline as raw material. The high activity trifluoroethyl sulfide intermediate. Based on the intermediate synthesized in the early stage, the urea and hydrazone modification routes are designed respectively: the urea group is introduced by classical reactions such as isocyanate method and urea condensation method to construct trifluoroethyl sulfide-urea bifunctional derivative; the hydrazone group is introduced by condensation reaction of carbonyl compound and phenylhydrazine to prepare hydrazone derivative containing trifluoroethyl sulfide structure and heterocyclic product. And it has good prevention and control effect on two-spotted spider mites, and has good agricultural application prospect.