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Synergistic application of farnesol to triazole fungicides in the control of smut

A technology of farnesol and smut, applied in the directions of fungicides, applications, biocides, etc., can solve the problems of long residence period and environmental pollution of pesticides, and achieve the effect of improving the bactericidal ability

Active Publication Date: 2020-07-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its long retention period in water and soil, serious pesticide residues can easily cause environmental pollution

Method used

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  • Synergistic application of farnesol to triazole fungicides in the control of smut
  • Synergistic application of farnesol to triazole fungicides in the control of smut
  • Synergistic application of farnesol to triazole fungicides in the control of smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] On the basis of the preliminary test, the farnesol and 10% difenoconazole microemulsion were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.05μM, 0.08μM, 0.1μM, 0.2μM, 0.3μM , 0.4μM, 0.5μM; 10% difenoconazole microemulsion (diluted): 1×10 5 Times, 5×10 4 Times, 3.34×10 4 times, 2×10 4 Times, 1.25×10 4 Times, 1×10 4 Times, 6.67×10 3 Times, 5×10 3 times). Pick a little sugarcane smut basidiospores from the plate and culture them overnight in 6mL YEPS culture medium as seed liquid, collect the bacteria by centrifugation the next day, and resuspend the bacteria with YEPS liquid medium (OD 600 0.2) for use. Draw 300 μL of mother solutions of different concentrations into 2 mL sterilized centrifuge tubes, add an equal amount of basidiospore suspension and mix well as a single use group; draw 250 μL of mother solutions of different concentrations of farnesol and 250 μL of azoxystrobin mother solutions and mix them in 5 m...

Embodiment 2

[0040] On the basis of the preliminary test, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.5 μM, 10% difenoconazole microemulsion: dilute 5000 times). Pick a little sugarcane smut basidiospores from the plate and culture overnight in 6mL YEPS culture solution as seed solution, transfer 500 μL to 50mL YEPS culture solution for expansion culture (to OD) the next day 600 0.2), draw 500 μL of different concentrations of mother solution into 5 mL sterilized centrifuge tubes, add an equal volume of bacterial solution and mix; then dilute 5000 times 10% difenoconazole microemulsion to 5 mL with 250 μL farnesol + 250 μL Add an equal volume of bacterial solution to a sterilized centrifuge tube and mix well; add 500 μL YEPS to a 5 mL sterilized centrifuge tube, add an equal volume of bacterial solution and mix well, and place in a shaker at 28°C and 200 rpm / min for 24 hours. Then use fr...

Embodiment 3

[0045] On the basis of the preliminary test, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a mother solution with a certain concentration gradient for later use (farnesol: 0.08 μM, 10% difenoconazole microemulsion: dilute 2×10 4 times). Pick a little sugarcane smut basidiospores from the plate and culture overnight in 6mL YEPS culture solution as seed solution, transfer 500 μL to 50mL YEPS culture solution for expansion culture (to OD) the next day 600 0.2) for use. Control the concentration of farnesol to 0.08μM, and dilute the concentration of difenoconazole to 2×10 4 times, and then set a gradient dilution sequence to 0.5 times, 0.4 times, 0.25 times, 0.1 times, 0.05 times of the mother solution. Take the same 250 μL of farnesol and the same volume of different concentrations of difenoconazole and mix them in a centrifuge tube, and use 500 μL of farnesol and azoxystrobin mother solutions as a single use group; 500 μL of YEPS as a blank cont...

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Abstract

The invention discloses new applicationfor fungicidal synergism of farnesol and triazole fungicides in prevention and treatment of smut of sugarcane, corn and other cropscaused by sporisorium and ustilago. Farnesol and triazole fungicides have obvious synergistic effects,can greatly reduce the use of chemical pesticides, have the prevention and treatment effect obviously superior to that of a single agent, and thus have a good application prospect.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the synergistic effect of farnesol and triazole fungicides, and is mainly used in the prevention and treatment of sugarcane smut and corn smut and the smut produced by Spore Sporisorium ) and Ustilago ( Ustilago ) caused by other crop smut, etc. Background technique [0002] Sugarcane is the main raw material of my country's sugar industry, and corn is the main food crop. They are also important feed crops and biomass energy materials. Disease infestation is a serious problem that plagues its development all the time. [0003] Sugarcane smut is caused by Ustilago sporogenes ( Sporisorium ) caused by infection. Corn smut is caused by the smut genus ( Ustilago ) caused by infection. These two pathogenic bacteria are dimorphic fungi and also belong to semi-specialized parasites. Haploids do not cause disease. They form dikaryotic mycelium through sexual cooperatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N31/02A01N43/653A01N25/04A01P3/00
CPCA01N25/04A01N31/02A01N43/653
Inventor 常长青仇善旭蔡恩平吴熔熔王雨婷李玲玉姜子德
Owner SOUTH CHINA AGRI UNIV
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