Novel method for rapidly identifying resistance level of sugarcane smut

A technology for smut and sugarcane, applied in the field of rapid identification of sugarcane smut resistance level, can solve the problems of inconsistent emergence rate and growth of sugarcane, human factors and complex environmental conditions, and inaccurate resistance evaluation results, etc., to achieve Short appraisal period, shortened experiment period, and saves the effect of harvesting

Pending Publication Date: 2022-03-25
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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  • Claims
  • Application Information

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Problems solved by technology

In addition, in the field management process of sugarcane in the later stage of planting, human factors and complex environmental conditions usually lead to inconsistencies in the emergence rate and growth of sugarcane
However, sugarcane smut resistance traits are quantitative traits and are easily affected by the environment. Therefore, using traditional methods, the resistance performance of the tested materials in different batches and different years often fluctuates greatly, resulting in inaccurate resistance evaluation results. Reliable evaluation results can only be obtained by carrying out multiple multi-point tests

Method used

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  • Novel method for rapidly identifying resistance level of sugarcane smut
  • Novel method for rapidly identifying resistance level of sugarcane smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A novel method for rapidly identifying the resistance level of sugarcane smut, characterized in that it comprises the following steps:

[0033] (2) Collection of sugarcane smut spores:

[0034] From May to August every year, the sugarcane smut whips are collected in the heavily diseased sugarcane areas. After the sugarcane smut whips are dried in a ventilated and dry room, the smut spore powder is collected;

[0035] (2) Preparation before cultivation:

[0036] The planting bucket is made of PVC material for cultivation. The specific specifications of the planting bucket are as follows: the height of the planting bucket is 40cm, the diameter of the bucket mouth is 25cm, and the sealing thickness of the bottom of the bucket is about 0.4cm; mouth 5cm;

[0037] (3) Planting:

[0038] Select the tested sugarcane varieties, take the single bud seed stems of the tested sugarcane varieties, plant 4 sugarcane buds in each planting bucket, plant 3-4 buckets of each material, ...

Embodiment 2

[0044] On the basis of Example 1, the varieties of sugarcane are: Q171 and ROC22; the experiment is designed according to random blocks, and two control varieties Q171 (disease resistance) and ROC22 (susceptibility) are set up for disease resistance and disease resistance; it is easy to carry out Test for identification of sugarcane smut resistance.

Embodiment 3

[0046] On the basis of Example 1-2, the collected smut spore powder was sieved with a 120-mesh sieve, and then stored in a refrigerator at 0°C; it was convenient for sugarcane smut spores to be collected and stored.

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Abstract

The invention discloses a novel method for rapidly identifying the resistance level of sugarcane smut, which comprises the following steps: selecting a mature plant which grows vigorously, cutting off sugarcane tips, and binding the tail ends of stems with a plastic film; the smut fungus teliospore suspension with the concentration of 5 * 10 < 6 > spores/m L is prepared from smut fungus teliospore with the germination rate of 90% or above. A medical injector is adopted to inject about 25 microliters of smut fungus teliospore (the concentration is 5 * 10 < 6 > spores/mL) suspension into each sugarcane bud of a plant, and about 90 buds are inoculated to each sugarcane material. After inoculation, the sugarcane material is still planted in a greenhouse and normally managed, the morbidity condition of sugarcane smut is investigated regularly, the total morbidity plant number is counted, and the final morbidity of each treatment is calculated. Positive control is set in the test, and resistance evaluation is carried out by meeting sugarcane smut resistance grading standards.

Description

technical field [0001] The invention relates to the technical field of sugarcane, in particular to a novel method for rapidly identifying the resistance level of sugarcane smut. Background technique [0002] Sugarcane smut is a kind of important sugarcane disease caused by Trichosanthes spp., which has been widely prevalent in the sugarcane areas of mainland my country. Infection of sugarcane with smut can reduce cane stalk yield and sucrose content, resulting in serious economic losses. Since it is difficult to control smut by general chemical or physical methods, it is generally considered that breeding smut resistant sugarcane varieties is the most economical and effective way to prevent smut. The evaluation of smut resistance of excellent parents and materials of sugarcane is the material basis for cultivating smut resistant varieties. [0003] There are many ways to identify sugarcane smut resistance. The most widely used method in the world to identify sugarcane smut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 胡鑫刘家勇夏红明徐超华吴转娣赵丽萍李纯佳赵培方李旭娟赵俊田春艳
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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