Method for improving yield of fruiting bodies of cordyceps takaomontana

Abstract

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for increasing the fruiting body yield of Cordyceps kaohsiung.

[0002] Background technique

[0003] Cordyceps takaomontana Yakush. & Kumaz. 1941 (= Isaria tenuipes peck (1879) =Isaria japonica Yasuda (1915) =Paecilomyces tenuipes (Peck) Samson (1974) = Paecilomyces japonica ) is an excellent Cordyceps resource, researches have found that it has obvious effects in anti-cancer, anti-depression, anti-insect, anti-bacterial, anti-virus and regulation of immunity. In particular, it has been discovered in recent years that the anticancer activity of one of its anticancer components, acetylepoxytrichothecene diol (4β-acetoxyscirpene-3α, 15-diol=AETD), is about greater than that of currently used clinical cancer patients. Cisplatin is 4-6 times stronger. Pharmacological tests show that the oral dose of Cordyceps Kaohsiung Cordyceps toxicity to mice is 70g / kg; the intra...

Examples

Embodiment 1

[0019] A method for increasing the fruiting body yield of Cordyceps kaoxiong, comprising the following steps:

[0020] (1) Preparation of fermentation broth of Zizania smut fungus GZUIFR-DX091

[0021] Use PDA medium to activate the Zizania smut GZUIFR-DX091 strain, pick 3 rings of the activated strain with an inoculation loop and insert it into the Zizania smut fermentation medium (boil 200g of potatoes for 30min and filter the juice, add 20g of glucose, Add water to 1000ml, 121°C, sterilize for 25min) into a 250ml Erlenmeyer flask with a liquid volume of 50ml, place it at 28°C, 160r / min for 5 days, centrifuge the culture solution at 5000r / min for 5min, and take the supernatant Liquid, promptly obtain the fermented liquid of Zizania zizania smut fungus GZUIFR-DX091 bacterial strain;

[0022] (2) Yield-increasing cultivation of Bombyx mori infected by Cordyceps Kaohsiung

[0023] a. Cordyceps Kaohsiung Cordyceps GZUIFR-PT211 strain infects silkworms: take the injection metho...

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for increasing the fruiting body yield of Cordyceps kaohsiung.

[0002] Background technique

[0003] Cordyceps takaomontana Yakush. & Kumaz. 1941 (= Isaria tenuipes peck (1879) =Isaria japonica Yasuda (1915) =Paecilomyces tenuipes (Peck) Samson (1974) = Paecilomyces japonica ) is an excellent Cordyceps resource, researches have found that it has obvious effects in anti-cancer, anti-depression, anti-insect, anti-bacterial, anti-virus and regulation of immunity. In particular, it has been discovered in recent years that the anticancer activity of one of its anticancer components, acetylepoxytrichothecene diol (4β-acetoxyscirpene-3α, 15-diol=AETD), is about greater than that of currently used clinical cancer patients. Cisplatin is 4-6 times stronger. Pharmacological tests show that the oral dose of Cordyceps Kaohsiung Cordyceps toxicity to mice is 70g / kg; the intra...

Examples

Embodiment 1

[0019] A method for increasing the fruiting body yield of Cordyceps kaoxiong, comprising the following steps:

[0020] (1) Preparation of fermentation broth of Zizania smut fungus GZUIFR-DX091

[0021] Use PDA medium to activate the Zizania smut GZUIFR-DX091 strain, pick 3 rings of the activated strain with an inoculation loop and insert it into the Zizania smut fermentation medium (boil 200g of potatoes for 30min and filter the juice, add 20g of glucose, Add water to 1000ml, 121°C, sterilize for 25min) into a 250ml Erlenmeyer flask with a liquid volume of 50ml, place it at 28°C, 160r / min for 5 days, centrifuge the culture solution at 5000r / min for 5min, and take the supernatant Liquid, promptly obtain the fermented liquid of Zizania zizania smut fungus GZUIFR-DX091 bacterial strain;

[0022] (2) Yield-increasing cultivation of Bombyx mori infected by Cordyceps Kaohsiung

[0023] a. Cordyceps Kaohsiung Cordyceps GZUIFR-PT211 strain infects silkworms: take the injection metho...