Screening and application of specific primers for scot-PCR of Ustilago cane whip

A sugarcane whip smut, specific technology, applied in the sugarcane whip smut SCoT-PCR specific primer screening and application field, can solve the problems that plant pathogenic fungi have not been reported, and achieve high polymorphism detection efficiency Effect
CN104673904BActive Publication Date: 2017-09-26SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2017-09-26

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Abstract

The invention belongs to the field of biotechnology, and specifically discloses a sugarcane smut SCoT-PCR (polymerase chain reaction) specific primer screening and an application. The primer sequence is shown as SEQ ID NO:1 to 10. Ten primers are used for amplifying ten sugarcane smut matching strains from different areas and hosts, totally, 86 bands are amplified, averagely, each primer amplifies 8.6 bands, the polymorphic rate is 59.3%. The screened specific primer has relatively high polymorphic detection efficiency, and can be used for genetic diversity analysis on sugarcane smut; and theoretical direction for selection of disease-resistant varieties of sugarcane is provided.
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Description

technical field

[0001] The invention relates to the field of biotechnology, and more specifically discloses the screening and application of SCoT-PCR specific primers for Ustilago cane whip. Background technique

[0002] sugar cane( Saccharum officinarum L.) is the most important sugar crop in the world and a renewable biomass energy crop with more development potential. by Ustilago cane whip ( Sporisorium scitamineum ) caused by sugarcane smut is an important sugarcane disease worldwide. At present, the main sugarcane varieties in my country are generally infected with smut, and the incidence rate in the field is high, and the economic loss is very serious. Breeding for disease resistance is the most effective and economical method to prevent and control sugarcane smut. However, the physiological races of S. smut in sugarcane areas in my country are not clear so far. There is a large blindness in breeding for resistance to smut in sugarcane. Breeding for resistance to s...

Claims

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