EST-SSR core primer group developed on basis of transcriptome sequence of towel gourd and application thereof

A technology of transcriptome sequence and core primers, which is applied in the field of EST-SSR core primer sets, can solve problems such as the weakness of loofah research, and achieve the effects of promoting healthy development, easy statistics, and good repeatability

Active Publication Date: 2014-08-27
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the weak basic theoretical research on loofah, there are currently two prominent problems in loofah breeding and variety protection: 1. How to accurately identify the genetic diversity of loofah germplasm resources, so as to efficiently guide breeders to select parents; 2. How to identify loofah varieties accurately and quickly, so as to better promote the development of loofah variety appr

Method used

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  • EST-SSR core primer group developed on basis of transcriptome sequence of towel gourd and application thereof
  • EST-SSR core primer group developed on basis of transcriptome sequence of towel gourd and application thereof
  • EST-SSR core primer group developed on basis of transcriptome sequence of towel gourd and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1. Development of core primer set for loofah EST-SSR

[0023] 1. Materials

[0024] Forty-six core germplasm resources (Table 1) were screened out from the loofah germplasm resources preserved in our unit for the evaluation of loofah EST-SSR core primer sets and the identification of resource genetic diversity. The 46 resource materials of loofahs include 36 ribbed loofahs and 10 ordinary loofahs; 25 of them are from China, 8 from Thailand, 7 from Malaysia, and 6 from unknown sources.

[0025] Table 1 is used for loofah resource genetic diversity analysis material information in the present invention

[0026]

[0027]

[0028] Note: - stands for unknown source.

[0029] 2. Extraction of loofah genomic DNA

[0030] Using the improved simple SDS method to extract loofah leaf genomic DNA, the steps are as follows:

[0031] (1) Take an appropriate amount of fresh leaves, put them into a 2ml centrifuge tube, add liquid nitrogen, grind the leaves into powder with a ...

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Abstract

The invention discloses an EST-SSR core primer group developed on the basis of the transcriptome sequence of towel gourd. The EST-SSR core primer group comprises 50 pairs of primers with nucleotide sequences as shown in SEQ ID No. 1-100 in a sequence table. The 50 pairs of EST-SSR core primers have the advantages of abundant polymorphism, stable amplification, good repeatability, convenience in statistics, etc.; meanwhile, since the primers are originated from the expressed gene of towel gourd and can reflect variation of functional sequences in a genome, so germplasm genetic diversity of towel gourd can be better distinguished. The core primer group can be used in fields like identification of the variety of towel gourd, genetic genealogical analysis of varieties and analysis of germplasm genetic diversity, is beneficial for protection of legal interests of breeders, producers and consumers and promotes sound development of genetic breeding and production of towel gourd.

Description

technical field [0001] The invention relates to an EST-SSR core primer set, in particular to a set of EST-SSR core primer sets developed based on loofah transcriptome sequences and applications thereof. Background technique [0002] Luffa is an annual climbing herb of Cucurbitaceae (Cucurbitaceae) Luffa spp. As an important vegetable crop, it has a wide planting area in the north and south of my country. In addition to being a vegetable, loofah is also an important medicinal plant. Modern pharmacology has proved that loofah contains a variety of active ingredients such as alkaloids, flavonoids, sterols, and glycosides, which have antifungal, antibacterial, and anti-inflammatory effects. Recent findings have demonstrated that loofah seeds contain ribosome-inactivating proteins that can inhibit the growth of the HIV virus and are therefore a potential drug for the treatment of AIDS. [0003] In recent years, as people pay more and more attention to nutrition and health care,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 吴海滨罗剑宁龚浩何晓莉罗少波郑晓明张长远
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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