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A kind of smut haploid strain um02 and its application

A technology of smut and UM02, which is applied in the direction of fungi, microorganisms, microorganisms, etc., can solve the problems of poor test accuracy, consistency and repeatability, uncertain results of artificial inoculation, and corn plant rot, etc., to achieve Strong pathogenicity, easy harvesting and transportation, and high success rate of inoculation

Active Publication Date: 2021-05-04
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "A Production Method and Product of Corn Mushrooms" (CN10540409593A) discloses a method of injecting an aqueous solution mixed with corn smut chlamydospores or basidiospores into the top of corn ears for inoculation to produce corn mushrooms. Although this method is simple to operate, However, because chlamydospores are easily mixed with other pathogenic bacteria and are not easy to find, direct inoculation with smut chlamydospore aqueous solution often causes the whole corn plant to rot and cause the test to fail; In order to reproduce in combination, two kinds of sexually compatible basidiospores must be mixed and inoculated together into the plant to be successful. However, the existing method is to directly make the basidiospore mixture after the chlamydospore germination into the inoculum for inoculation. The randomness of the mixture does not necessarily guarantee that the inoculum contains two sexually compatible haploid strains, which leads to great uncertainty in the results of artificial inoculation and the accuracy, consistency and repeatability of the test. very bad

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  • A kind of smut haploid strain um02 and its application
  • A kind of smut haploid strain um02 and its application
  • A kind of smut haploid strain um02 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1 Isolation, screening and identification of Ustilago maize haploid strain of the present invention

[0039] Proceed as follows:

[0040] (1) Collect teliospores and prepare teliospore suspension: Naturally air-dried corn smut galls were collected in Hainan in December 2016, and a small amount of teliospores were directly picked from the corn smut galls and placed in a sterilized 2ml centrifuge tube, then add 1ml of sodium hypochlorite solution with a weight percentage of 1%, mix it upside down for 2min, centrifuge at 12000rpm for 1min, remove the supernatant, add sterilized water to wash the precipitate 3 times, and finally dilute the precipitate with sterile water. Promptly get teliospore suspension, its final concentration is 10 3 spores / ml.

[0041](2) Teliospore culture: Take 200 μl of the teliospore suspension obtained in step (1) and spread it on PDA medium, and cultivate it at 25° C. for 2 days, and then small colonies visible to the naked eye can b...

Embodiment 2

[0051] Example 2 Taxonomic identification of the haploid strain UMO2 of the present invention

[0052] (1) Morphological characteristics of the strain: the spores of the strain are short rod-shaped, without septum, and the length is 15-25 μm.

[0053] (2) Culture characteristics of the bacterial strain: on the PDA plate medium, the bacterial colonies are milky white, with wrinkles on the surface, moister, similar to yeast colonies, but more viscous than bacteria and yeast colonies when picked.

[0054] (3) Molecular identification of haploid bacterial strain UM02 of the present invention:

[0055] Using the genomic DNA of the UM02 bacterial strain as a template, the fungal genome ITS segment was amplified by PCR using fungal universal primers ITS1 and ITS4, and the primer sequences were:

[0056] ITS1: 5'-TCCGTAGGTGAACCTGCGG-3';

[0057] ITS4: 5'-TCCTCCGCTTATTGATATGC-3'.

[0058] The PCR reaction system (25 μL), wherein: ITS1 (10 μmol / L) 0.5 μL, ITS4 (10 μmol / L) 0.5 μL, Es ...

Embodiment 3

[0060] Embodiment 3 The preparation of the haploid strain UMO2 inoculation mother solution of the present invention

[0061] Proceed as follows:

[0062] (1) Strain activation: Inoculate the UM02 bacterial strain preserved at low temperature on PDA plate medium (the preparation method is: peel 200g of potatoes and cut them into small pieces, put them into 800ml of distilled water and boil for 20min, filter with 4 layers of gauze, Add 20 g of glucose and 20 g of agar, boil to 1000 ml, sterilize at 121° C. for 20 min), and cultivate for 1 day at 25° C. in the dark to obtain activated strains.

[0063] (2) Bacteria preparation: use an inoculation needle to pick an appropriate amount of the activated UMO2 strain obtained in step (1) and spread it evenly on the PDA plate medium, and culture it at 25°C in the dark for 3 days to obtain UMO2 bacteria.

[0064] (3) Preparation of inoculated mother solution: scrape off all the thallines obtained in step (2) from the PDA plate culture m...

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Abstract

The invention discloses a haploid strain UM02 of Ustilago maydis, and its preservation number is CGMCC No.15387. The haploid strain UM02 of maize smut haploid strain UM02 of the present invention is combined with the haploid strain matched with its mating type to have strong pathogenicity, high success rate of inoculation, accurate and reliable identification results of maize variety resistance to maize smut resistance, and Good consistency; when used in the production of corn mushrooms, not only the yield of corn mushrooms is high, but also wrapped in bracts, which is convenient for harvesting and transportation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a haploid strain U02 of Ustilago maize, and also relates to the application of the strain. Background technique [0002] Corn smut (or corn smut) is a corn disease caused by corn smut (Ustilago maydis, or called corn smut, corn smut, etc.), mainly occurs in warm and dry areas, And the spring sowing area is more serious than the summer sowing area, the general incidence rate is 5-10%, and serious ones can cause 30-80% yield loss, which is a problem to be solved urgently. [0003] In addition to causing harm to corn, the galls formed on the ears of corn after being infected by smut fungus are also a delicious food. The Aztecs in Mexico have eaten them for more than 600 years. They are called "Mexican truffles." "In our country, it is called corn mushroom, corn black mold, corn smut, corn black mold, stick bag, rentou, black rice or black gallbladder, etc. Corn mushrooms c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/04C12Q1/18C12R1/645
CPCC12N1/14C12Q1/04C12Q1/18C12N1/145C12R2001/645
Inventor 张家齐石洁张海剑杨硕郭宁金戈刘树森
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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