Ribose culture medium applicable to ustilaginoidea virens spore production and application method of ribose culture medium

Abstract

The invention discloses a ribose culture medium applicable to ustilaginoidea virens spore production and an application method of the ribose culture medium. Ribose which is not sterilized at high temperature is adopted to prepare a potato ribose culture medium as a spore production culture medium for culturing and preparing ustilaginoidea virens thin-wall conidia. The preparation and application method of the culture medium comprises the following operation steps: 1) preparing a basic culture medium; 2) filtering and sterilizing ribose; 3) preparing a ribose culture medium plate; 4) planting ustilaginoidea virens; 5) performing constant-temperature culture; and 6) collecting a new generation of spores. The ribose culture medium has the characteristics and advantages that the time for obtaining a great amount of spores can be shortened, the continuous spore production time is long, operation techniques are simple, convenient and rapid, no oscillation culture equipment is needed, a relatively great number of spores of the new generation can be produced, and a spore liquid is relatively pure.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it relates to a ribose culture medium suitable for spore production of rice smut fungus and an application method thereof. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut (caused by Ustilaginoideavirens (Cooke) Takahashi infection) has become a major disease of rice ...

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Problems solved by technology

However, there are also some obvious deficiencies in the technology of preparing spores with this medium. After the sporulation peak, a large number of non-sporulation aerial hyphae grow quickly on the plate colony, resulting in a sharp decline in the number of spores, which cannot be produced in a long period of time. Stable provision of spores for use; moreover, there are still more fragments of mycelium in the new spore liquid collected for preparation of culture results

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