Drying and sealed preservation method of rice ustilaginoidea virens

Abstract

The invention discloses a drying and sealed preservation method of rice ustilaginoidea virens. The method technologically utilizes the property that the ustilaginoidea virens can survive in completely dried rice endosperm for a long period; rice is used as a carrier and the ustilaginoidea virens is artificially inoculated onto sterile rice grains and hyphae fully cover endosperm tissues of the rice grains; after the rice grains are completely dried under the condition of 28 DEG C, a drying preservation manner is adopted. The method comprises the following technical steps: 1) obtaining purified ustilaginoidea virens strains by utilizing a chlamydospore single-spore isolation method; 2) immersing the rice grains until the endosperm is fully filled with water; loading the endosperm into a test tube and sterilizing; 3) inoculating the endosperm onto the sterile rice grains and culturing to enable the hyphae fully cover the inner parts of the endosperm tissues; drying until the rice grains are completely dried, preserving the test tube into a dryer filled with allochroic silica gel and preserving for a long period; smearing vaseline for sealing. The drying and sealed preservation method has the advantages that the preservation time of the ustilaginoidea virens exceeds 5 years; the survival rate is high; the pollution rate of the strains is low; the operation is convenient and a small space is occupied; the cost is low and energy sources are not consumed; the pollution to environment and residues are not caused.

Description

technical field [0001] The invention relates to a long-term dry storage method for rice smut bacteria, which belongs to the research field of biological characteristics and control of microorganisms. Background technique [0002] Rice is the most important food crop, which has long been affected by rice diseases on yield and quality. With the long-term promotion and planting of hybrid rice with a relatively single genetic background and the large-scale application of nitrogen fertilizers, rice false smut has risen from a minor disease in the past to a major rice disease, affecting and restricting the development of the rice industry. The rice false smut can also produce Toxins that are toxic to humans and animals, so the occurrence of this disease has a relatively serious impact on the yield and quality of rice. However, due to the conventional storage method of rice smut fungus, it is easy to be contaminated by other microorganisms during long-term storage, and the surviva...

AI Technical Summary

Problems solved by technology

However, due to the conventional storage method of rice smut fungus, it is easy to be contaminated by other microorganisms during long-term storage, and the survival time is short, which restricts the correlation between the biological characteristics, genetic diversity analysis and pathogenicity change characteristics of the rice smut fungus in different years. Research

Images