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Ustilaginoidea virens PCR detection primers and application thereof

A technology for detecting primers and Aspergillus oryzae, which is applied in the field of PCR detection primers for Aspergillus oryzae, which can solve the problems of low specificity of detection primers and single detection target, and achieve the effects of good specificity, single detection target and strong stability

Inactive Publication Date: 2016-09-28
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of traditional oryza koji PCR specificity detection target is single, detection primer specificity is not strong, a kind of rice koji fungus specific nested PCR detection primer with strong specificity and high sensitivity is provided, the present invention Uses of the primer are also provided

Method used

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  • Ustilaginoidea virens PCR detection primers and application thereof
  • Ustilaginoidea virens PCR detection primers and application thereof
  • Ustilaginoidea virens PCR detection primers and application thereof

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Embodiment 1

[0024] 1. Aspergillus oryzae whole genome prediction, specific detection target screening and primer design

[0025] 1.1 Whole genome prediction of Aspergillus oryzae

[0026] According to the HWD-2 genome of Aspergillus oryzae sequenced strain obtained by sequencing (Jia et al., 2015), the AUGUSTUS (Version 2.5.5) software was used to use the Fusarium graminearum genome as a reference model for the gene analysis of Aspergillus oryzae. prediction. The final prediction and analysis of Aspergillus oryzae contains 8231 genes, and the corresponding DNA sequence and protein sequence are obtained.

[0027] 1.2 Construction of local database for comparison and screening of specific target genes of Aspergillus oryzae

[0028] In order to reduce the amount of computer calculations, reduce the computer configuration requirements and save time, two databases are used, one is a database containing 43 species predicted protein sequences (Table 1), and the other is NCBI's NR database, with BLASTP ...

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Abstract

The invention discloses a group of ustilaginoidea virens PCR detection primers. Specific detection targets of ustilaginoidea virens are found through a method for comparing genomes, and the detection primers are designed. Compared with traditional ustilaginoidea virens PCR detection primers, the detection primers have the advantages of being good in specificity, high in stability and high in sensitivity, and the false positive phenomenon is well controlled in the detection process; meanwhile, ustilaginoidea virens can be accurately detected, and good tools are provided for field detection of ustilaginoidea virens, circulation research of ustilaginoidea virens diseases, prediction of the ustilaginoidea virens diseases and the like.

Description

Technical field [0001] The invention belongs to the field of biology, and specifically relates to a PCR detection primer for Aspergillus oryzae and its use. Background technique [0002] Rice false smut (Rice false smut) is an important rice fungal disease caused by Ustilaginoidea virens, which occurs in all rice regions around the world. In recent years, with the changes in rice cultivation patterns, the increase in the amount of nitrogen fertilizer applied in the paddy field, and the large-scale promotion of new high-yielding dense panicle hybrid rice, rice smut disease has gradually risen to the main disease of rice. The large-scale occurrence of rice smut not only affects the yield and quality of rice, but the rice aspergillin produced in rice smut has a toxic effect on humans and animals and seriously affects food security. [0003] Current research suggests that Aspergillus oryzae overwinters in soil or diseased bodies with chlamydospores and sclerotia, germinates to form co...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895
Inventor 郑露汤近天黄俊斌张耀
Owner HUAZHONG AGRI UNIV
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