Anti-ustilaginoidea virens major gene and molecular marker thereof

A technology of molecular markers and rice false smut, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as gene linkage, achieve the effect of reducing breeding scale and improving breeding efficiency

Active Publication Date: 2013-04-03
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

The patent applicant used the backcross introduction line population composed of 213 lines derived from the disease-resistant parent Lemont and the susceptible parent Teqing to identify the resistance of each line to rice false smut through natural induced inoculation in the field for two consecutive years. Two new resistance loci, qFSR10.2 and qFSR10.5, were found on chromosome 10, and qFSR10.2 was located in the small interval RM222-RM216, but it may be linked to genes controlling heading date in rice

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  • Anti-ustilaginoidea virens major gene and molecular marker thereof
  • Anti-ustilaginoidea virens major gene and molecular marker thereof
  • Anti-ustilaginoidea virens major gene and molecular marker thereof

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with specific implementation examples. The following examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention. Without departing from the essence of the present invention, any replacement of the methods, steps or recipient parents of the present invention falls within the scope of the present invention. The experimental methods in the following implementation examples are conventional methods unless otherwise specified.

[0020] 1. Positioning population and identification of resistance

[0021] Teqing background backcross introduction line population consists of 213 lines, including 133 BC 2 f 5 and 80 BC 3 f 4 strain. Teqing is a high-yield indica rice variety widely promoted in my country, and Lemont is a high-quality, high-yield japonica rice variety popularized in the southern United States.

[0022] In the ric...

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Abstract

The invention relates to a molecular marker of a new anti-ustilaginoidea virens gene of rice, belonging to the field of rice breeding for disease resistance. According to the invention, each strain of Teqing background backcross introgression lines obtained by the cross and backcross between a susceptible parent Teqing and a disease-resistant parent Lemont is subjected to evaluation of disease resistance and molecular genetic linkage analysis and positioned to the anti-ustilaginoidea virens gene qFSR10.5 on the 10th chromosome of rice; and the molecular marker linked with the qFSR10.5 is RM258. By checking the RM258 banding pattern data and screening the ustilaginoidea virens resistance controlled by the qFSR10.5 resistance allele, the breeding efficiency of the anti-ustilaginoidea virens rice variety can be improved.

Description

technical field [0001] The invention relates to a molecular marker of a new rice smut resistance gene qFSR10.5, belongs to the field of rice disease resistance breeding, and is suitable for assisting selective breeding and aggregation breeding of the above-mentioned gene for rice smut disease by molecular markers. Background technique [0002] Rice smut (Ustilaginoidea virens (Cooke) Tak.) is an important fungal disease of rice. The fungus transforms rice grains into rice balls, covered with a large number of powdery, dark green chlamydospores. Since the 1980s, with the popularization of high-yield varieties bred by hybrid rice and indica-japonica crosses and the application of corresponding yield-increasing measures, rice false smut has risen from a minor disease to a major disease in my country, and is currently spreading across all major rice planting areas. Rice false smut significantly reduces rice yield and rice quality. In severe years, the yield loss rate reaches 30...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 周永力谢学文张帆徐建龙王韵黎志康
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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