The invention discloses a remote I/O (input/output) control system of a Modbus fieldbus DI-16-route monitor, which is characterized in that a process of carrying out message transmission (which is implemented by using a fieldbus in an RTU (remote terminal unit) mode of a Modbus master/slave station protocol) between a master station and a slave station comprises the following steps of: sending a request message frame by a master station (6) U0; reading the request message frame by terminals J1 and U3, a transceiver drive communication circuit (5), a transceiver message control circuit (4) and a slave station (3) U1; acquiring on-off data by a subprogram of the U1; sending response message frames by the terminal J1, an on-off acquisition circuit (1), a photoncoupled isolating circuit (2) and the slave station (3) U1; and reading the response message frames by the transceiver message control circuit (4), the transceiver drive communication circuit (5), the terminal J1 and the master station (6) U0. The DI-16-route monitor is a slave station product for filed on-off acquisition, and provided with a PLC (programmable logical controller) product which is in a structure of integrating a power supply (with a visual diagnosis function and an anti-electromagnetic interference capability), a CPU (central processing unit) and I/O nodes, and compact in appearance, and easy to install, wiring and debug.