The present invention relates generally to decellularized 
extracellular matrix of conditioned body tissues. The decellularized 
extracellular matrix contains a biological material, preferably 
vascular endothelial growth factor (VEGF), produced by the conditioned 
body tissue that is in an amount different than the amount of the biological material that the 
body tissue would produce absent the conditioning. The invention also relates to methods of making and methods of using said decellularized 
extracellular matrix. Specifically, the invention relates to treating defective, diseased, damaged or ischemic cells, tissues or organs in a subject by administering, injecting or implanting the decellularized 
extracellular matrix of the invention into a subject in need thereof. The invention is further directed to a tissue regeneration 
scaffold for implantation into a subject inflicted with a 
disease or condition that requires tissue or organ repair, regeneration and / or strengthening. Additionally, the invention is directed to a 
medical device, preferably a 
stent or an 
artificial heart, having a surface coated or covered with the decellularized 
extracellular matrix of the invention or having a component comprising the decellularized 
extracellular matrix of the invention for implantation into a subject, preferably a human. Methods for making the tissue regeneration 
scaffold and methods for manufacturing a coated or covered 
medical device having a component comprising decellularized extracellular matrix of conditioned body tissues are also provided.