Wettable powder of trichoderma asperellum DQ1 composition and application thereof

A technology of wettable powder and Trichoderma aculeatus, which is applied in application, biocide, animal repellent, etc., can solve the problems of environmental pollution, toxicity to humans and animals, and residues, so as not to pollute the environment, and promote absorption and growth , Improving the effect of rice product quality

Inactive Publication Date: 2018-06-22
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An object of the present invention is to provide a kind of wettable powder of Trichoderma aculeatus DQ1 composition, the wettable powder of this Trichoderma aculeatus DQ1 composition solves the problem that the chemical pesticides currently used produce when c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The wettable powder of the Trichoderma aculeatus DQ1 composition is prepared by the following preparation method:

[0018] Step 1: Inoculate Trichoderma aculeatus DQ1 strain on PDA slant medium, culture at 28°C for 72 hours, wash the conidia with sterilized distilled water to make a spore liquid, the number of spores in the spore liquid is 10 6 Pieces / mL, then add 1% (volume ratio) to sterilized PD culture solution, put it in 28℃, 120rpm culture for 36h to obtain first-level seed culture solution.

[0019] Step 2: Mix the bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio). After stirring, fill it into a 200mL plastic bottle. The filling amount is 1% of the height of the plastic bottle. / 2, then autoclave at 121°C for 20 minutes, put the first-level seed culture solution into a plastic bottle according to 1% of the inoculum amount after cooling, shake it up and down evenly, place it at a temperature of 28°C and a humidity of 80% and incubate for ...

Embodiment 2

[0025] The wettable powder of the Trichoderma aculeatus DQ1 composition is prepared by the following preparation method:

[0026] Step 1: Inoculate Trichoderma aculeatus DQ1 strain on PDA slant medium, culture at 28°C for 96 hours, wash the conidia with sterilized distilled water to make a spore liquid, the number of spores in the spore liquid is 10 6 Pieces / mL, then add 1% (volume ratio) into the sterilized PD culture solution, and incubate at 28°C and 120rpm for 40h.

[0027] Step 2: Mix the bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio). After stirring, fill it into a 200mL plastic bottle. The filling amount is 1% of the height of the plastic bottle. / 2, then autoclave at 121℃ for 20 minutes, put it in a plastic bottle at 1% of the inoculum after cooling, shake it up and down, place it at a temperature of 28℃, and a humidity of 80% for 7 days; According to the mass ratio: 1:800, add sterile water, stir evenly to make a spore suspension, and the...

Embodiment 3

[0033] The wettable powder of the Trichoderma aculeatus DQ1 composition is prepared by the following preparation method:

[0034] Step 1: Inoculate the DQ1 strain of Trichoderma aculeatus in PDA slant medium, culture at 28°C for 84 hours, wash the conidia with sterilized distilled water to make a spore liquid, the number of spores in the spore liquid is 10 6 Pieces / mL, then add 1% (volume ratio) into the sterilized PD culture solution, and incubate at 28°C and 120rpm for 48h.

[0035] Step 2: Mix the bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio). After stirring, fill it into a 200mL plastic bottle. The filling amount is 1% of the height of the plastic bottle. / 2, then autoclave at 121°C for 20 min. After cooling, insert 1% of the inoculum into a plastic bottle, shake it up and down, place it at a temperature of 28°C and a humidity of 80%, and incubate for 7 days; The seeds are added to sterile water at a mass ratio of 1:1000 and stirred evenly to...

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Abstract

The invention relates to wettable powder of a trichoderma asperellum DQ1 composition and application thereof. The wettable powder of the trichoderma asperellum DQ1 composition is prepared from the following methods: one, preparing the trichoderma asperellum DQ1 strain as spore liquid to culturing; two, manufacturing the spore suspension, wherein the spore suspension is used as solid tray fermentation seed liquid; three, mixing the sterilized fermentation substrate and the solid tray fermentation seed liquid, culturing, air-drying, and crushing to obtain the trichoderma asperellum DQ1 spore powder; four, mixing the trichoderma asperellum DQ1 spore powder, the validamycin with raw powder content of 60%, and 5000UI/microgram bacillus thuringiensis in mass ratio of 10:(0.5-1):(0.5-1) to prepare the trichoderma asperellum DQ1 composition; and five, preparing the trichoderma asperellum DQ1 as the wettable powder. The wettable powder disclosed by the invention can effectively play the pesticide effect of the biopesticide, and can inhibit and kill banded sclerotial blight and ustilaginoidea virens and other plant diseases and insect pests, is non-toxic to the human and the animal and freefrom environment pollution.

Description

1. Technical Field [0001] The invention relates to a biological pesticide for the prevention and control of rice diseases and insect pests, in particular to a wettable powder of Trichospora aculeatus DQ1 composition and its application. 2. Background technology [0002] In recent years, there have been many types of rice diseases and insect pests in my country, with serious damage, especially rice sheath blight, rice smut, rice leaf roller and stem borer, which seriously threaten the safety of rice production in my country. However, the current prevention and control of rice pests and diseases mainly rely on chemical means. It is well known that chemical pesticides have residues in crops, air, water and soil, causing excessive pesticide residues and environmental pollution, and the use of a single agent can easily cause pests and diseases to develop resistance and kill them. Some beneficial microorganisms in the soil reduce the soil beneficial microorganisms, reduce the soil orga...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/14A01P3/00A01P7/04
CPCA01N25/14A01N63/30
Inventor 刘铜侯巨梅刘震
Owner HAINAN UNIVERSITY
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