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Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases

A technology of TA-9 and Trichoderma dark green, applied in the field of microorganisms, can solve the problems of affecting food safety, the effect of drug resistance of rice sheath blight, the decline and the like, and the fermentation process is simple and feasible, which is conducive to long-term storage. , the effect of high content

Active Publication Date: 2015-05-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Jinggangmycin has been used in excessive single-use for a long time to control rice sheath blight, and it has been reported that rice sheath blight bacteria have developed drug resistance and the control effect has declined in some areas.
[0004] Rice false smut has occurred in all major rice areas in my country, and has become one of the important diseases on rice. Its pathogenic bacteria is Ustilaginoidea virens, which can invade grains to form rice bent balls and produce toxic to humans and animals. Toxins, reduce rice yield and quality, affect food safety

Method used

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  • Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases
  • Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases
  • Trichoderma atroviride Ta-9 strain and application thereof in prevention and control of rice diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Method for isolating and obtaining Trichoderma atroviride TA-9 strain.

[0027] The PDA medium formula is: potato 200g / L, glucose 20g / L, water 1L, agar 20g / L (solid medium). Peel the potato and cut into small pieces, add 1L of water and boil for 30min, filter with 2 layers of gauze, add glucose to the filtrate, add water to make up to 1L, if you need to make a solid medium, add agar and boil it. After aliquoting, 121℃ humid and hot Sterilize.

[0028] It includes the following steps:

[0029] (1) Strain isolation, purification and preservation. Collected in many rice fields in Nanjing City, there are 200 rice curds scattered in the fields 7-10 days after harvest. After being dried in the shade, they are half buried in humid sterilized quartz sand and cultivated at 28°C for 15-20 days. The parasitic fungus grows on the surface of the rice curve ball. Carefully pick the parasitic fungus on the surface of the rice curve ball onto a PDA plate containing 50μg / mL chlor...

Embodiment 2

[0034] Example 2: Preparation of chlamydospore and conidia fermentation product of Trichoderma aeruginosa TA-9 strain.

[0035] Transfer Trichoderma dark green TA-9 to the PDA slant medium and incubate at 28-30℃ for 7 days; wash the conidia on the PDA slant medium with sterile water, and adjust the concentration of the spore solution after counting with a hemocytometer To 1.0×10 6 Pieces / mL; prepare liquid fermentation medium, its components are: 15g corn flour, 20g oat flour, adjust the pH to 5.0, make the volume to 1L with water, put each 200mL into a 1L triangular culture flask, and sterilize with moist heat at 121°C for 30 minutes . Add 2 mL of the above-mentioned conidia spore liquid to every 200 mL of liquid fermentation medium, and shake culture for 7-9 days at 30°C and 160r / min under dark conditions. After the fermentation culture is broken up, the content of 5.4×10 is obtained. 7 Chlamydospores / mL and 1.6×10 8 The fermentation broth of conidia per mL can be diluted and a...

Embodiment 3

[0036] Example 3: The control effect of the fermented product of Trichoderma dark green TA-9 on rice sheath blight.

[0037] This experiment was carried out on potted rice. The seedlings of the bred rice variety Jingang 30 were transplanted into pots (diameter 30cm, height 40cm), 3 holes per pot, 5 seedlings per hole, fertilizer and water management in accordance with conventional management, and ensure that the fertilizer and water conditions of each pot are consistent.

[0038] The 5mm diameter Rhizoctonia solani wild-type strain RH-2 was inoculated onto a PDA plate, cultured at 28°C for 3-4 days, the medium was chopped and inoculated into an Erlenmeyer flask containing sterilized matchsticks. Continue to incubate at 28°C for 3-4 days, during which time, shake and mix once a day to allow Rhizoctonia solani hyphae to attach to loose matchsticks.

[0039] The matchsticks with Rhizoctonia solani were inserted into the sheaths of the last 3-4 leaves of the rice at the tillering stage,...

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Abstract

Trichoderma atroviride Ta-9 has the collection number of CGMCC No. 9698 and is collected at the CGMCC (China General Microbiological Culture Collection Center). The trichoderma atroviride Ta-9 disclosed by the invention can be used for preventing and controlling a variety of rice diseases as a biological pesticide. The trichoderma atroviride Ta-9 shows relatively strong superparasitism ability to rhizoctonia solani and ustilaginoidea virens. The invention further relates to trichoderma atroviride Ta-9 high-yield chlamydospore and conidiospore liquid fermentation technologies. After fermentation liquor of the trichoderma atroviride Ta-9 is diluted by 50 times, the diluted fermentation liquor has relatively good prevention and control action on both rice sheath blight disease caused by rhizoctonia solani and green smut caused by ustilaginoidea virens.

Description

Technical field [0001] The invention belongs to the field of microorganisms, and relates to a Trichoderma atroviride TA-9 strain and its application in the prevention and control of rice diseases. technical background [0002] At present, the use of chemical pesticides is one of the main measures for the prevention and control of crop diseases. Chemical pesticides have played an important role in improving the yield and quality of agricultural products, but the extensive use of chemical pesticides has also led to a series of problems such as environmental pollution, ecological damage, pesticide residues in agricultural products, and resistance to pathogens. Making full use of comprehensive prevention and control measures to prevent and control plant diseases and reduce the use of chemical pesticides has become the consensus of plant protection workers. The use of beneficial microorganisms in the environment to prevent and control plant diseases is one of the hot research directi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 于俊杰刘永锋孟祥坤
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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