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A trichoderma ta-9 strain and its application in rice disease control

A technology for trichoderma dark green and rice disease, applied in the field of microorganisms, can solve the problems affecting food safety, decline, and the control effect of rice sheath blight resistance, and achieves high content, simple and easy fermentation process, and is beneficial to long-term The effect of time storage

Active Publication Date: 2017-11-17
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Jinggangmycin has been used in excessive single-use for a long time to control rice sheath blight, and it has been reported that rice sheath blight bacteria have developed drug resistance and the control effect has declined in some areas.
[0004] Rice false smut has occurred in all major rice areas in my country, and has become one of the important diseases on rice. Its pathogenic bacteria is Ustilaginoidea virens, which can invade grains to form rice bent balls and produce toxic to humans and animals. Toxins, reduce rice yield and quality, affect food safety

Method used

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  • A trichoderma ta-9 strain and its application in rice disease control
  • A trichoderma ta-9 strain and its application in rice disease control
  • A trichoderma ta-9 strain and its application in rice disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: A method for isolating and obtaining Trichoderma atroviride TA-9 strain.

[0027] The formula of PDA medium is: potato 200g / L, glucose 20g / L, water 1L, agar 20g / L (solid medium). Peel the potatoes and cut them into small pieces, add 1L of water and boil for 30 minutes, filter with 2 layers of gauze, add glucose to the filtrate, add water to make up to 1L, add agar to make a solid medium and boil, divide and heat at 121°C Sterilize.

[0028] Include the following steps:

[0029] (1) Strain isolation, purification and preservation. A total of 200 rice balls were collected in many rice fields in Nanjing City 7-10 days after harvesting and scattered in the field. After drying in the shade, they were half-buried in moist sterilized quartz sand and cultured at 28°C for 15-20 days. The parasitic fungi grow on the surface of the rice ball. Carefully pick the parasitic fungi on the surface of the rice ball and place them on a PDA plate containing 50 μg / mL chloramph...

Embodiment 2

[0034] Example 2: Preparation of chlamydospore and conidia fermented product of Trichoderma dark green TA-9 strain.

[0035] Move Trichoderma TA-9 into the PDA slant medium and culture it at 28-30°C for 7 days; wash the conidia on the PDA slant medium with sterile water, and adjust the concentration of the spore liquid after counting with a hemocytometer to 1.0×10 6 pcs / mL; Prepare liquid fermentation medium, its components are: 15g corn flour, 20g oat flour, adjust pH to 5.0, dilute to 1L with water, put each 200mL into 1L Erlenmeyer flask, sterilize with moist heat at 121℃ for 30min . Add 2mL of the above-mentioned conidia spore liquid to every 200mL of liquid fermentation medium, shake culture at 30°C and 160r / min for 7-9d under dark conditions, and break up the fermentation culture to obtain 5.4×10 7 Chlamydospores / mL and 1.6×10 8 The fermentation broth of conidia per mL can be diluted and added with surfactant for spray application.

Embodiment 3

[0036] Example 3: Control effect of Trichoderma dark green TA-9 fermentation product on rice sheath blight.

[0037] This experiment was carried out on potted rice. Transplant the seedlings of the well-raised rice variety King Kong 30 into pots (30 cm in diameter and 40 cm in height), plant 3 holes in each pot, and 5 seedlings in each hole. The fertilizer and water are managed according to the routine, and the fertilizer and water conditions of each pot are guaranteed to be consistent.

[0038] Inoculate the dish of Rhizoctonia solani wild-type strain RH-2 with a diameter of 5mm on a PDA plate, culture at 28°C for 3-4 days, cut up the medium and inoculate it into a Erlenmeyer flask equipped with sterilized matchsticks. Continue culturing at 28°C for 3-4 days, and shake and mix once a day during this period, so that the hyphae of Rhizoctonia solani can be attached to loose matchsticks.

[0039] The matchstick with Rhizoctonia solani was inserted into the leaf sheath of the pen...

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Abstract

A kind of Trichoderma atroviride TA‑9, which has been preserved in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.9698. The Trichoderma atroviride TA-9 of the present invention can be used as a biological pesticide to prevent and control various rice diseases. Trichoderma dark green TA‑9 showed strong hyperparasitic ability against Rhizoctonia solani and Ustilaginoidea virens. The present invention also relates to the high-yield chlamydospore and conidia liquid fermentation technology of Trichoderma dark viridans TA-9, after its fermented liquid is diluted 50 times, it can control rice sheath blight caused by Rhizoctonia solani and rice sheath blight caused by Chlorosclerotinum oryzae. Aspergillus has a good control effect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Trichoderma atroviride TA-9 and its application in rice disease prevention and control. technical background [0002] At present, the use of chemical pesticides is one of the main measures for crop disease control. Chemical pesticides have played an important role in improving the yield and quality of agricultural products, but the extensive use of chemical pesticides has also led to a series of problems such as environmental pollution, ecological damage, pesticide residues in agricultural products, and drug resistance of pathogenic bacteria. It has become the consensus of plant protection workers to make full use of comprehensive prevention and control measures to prevent and control plant diseases and reduce the use of chemical pesticides. The use of beneficial microorganisms in the environment to prevent and control plant diseases is one of the hot research directions....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 于俊杰刘永锋孟祥坤
Owner JIANGSU ACAD OF AGRI SCI
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