A preparing method of a hyperparasitic fungal inoculant for sclerotinia sclerotiorum

A technology of fungal agent and sclerotinia, applied in botany equipment and methods, biocides, fungicides, etc., can solve the problems of food safety impact, unsatisfactory control effect, high cost, etc.

Inactive Publication Date: 2015-01-28
JIANGSU TIANXIANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of Sclerotinia mainly relies on chemical pesticides, but chemical control is not only costly, pollutes the environment, but also has unsatisfactory control effects; at the same time, food safety is also seriously affected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation culture:

[0024] Collect rhizosphere soil samples, add sclerotia to 30 grains / 100 g soil samples and mix well, trap parasites at a constant temperature of 25°C; take them out after 3 weeks, rinse with tap water, sterilize the surface with 1% NaCIO for 1 minute, and fully sterilize with sterile water. After rinsing, put it on a filter paper sheet placed in a petri dish, and after 2 weeks of moisturizing culture at 25°C, check the hyperparasites on the surface of the sclerotium under a stereoscope, pick out monospores and culture them on a PDA plate, and purify and separate them.

[0025] Liquid fermentation:

[0026] Test tube culture: use solid potato grape agar (PDA) medium, inoculate the hyperparasitic fungal strain on the test tube medium, and culture at 26°C for 4 days;

[0027] Expansion culture: use liquid potato dextrose (PD) medium to inoculate the hyperparasitic fungal strains in the test tubes into Erlenmeyer flasks, place them on a shaker at 26°C ...

Embodiment 2

[0032] The process steps are as in Example 1, the rhizosphere soil sample is collected in the separation and cultivation step, sclerotia is added in 40 grains / 100 g of the soil sample and mixed evenly, parasitic bacteria are trapped at a constant temperature of 25°C, and taken out after 3.5 weeks. In the liquid fermentation step, the fermentation culture uses glucose and urea as carbon and nitrogen sources respectively, and the C / N ratio is 12:1. In the liquid fermentation step, the culture in the Erlenmeyer flask is added to the fermentation broth at a ratio of 7%. In the preparation step of the bacterial agent, the bacterial slurry obtained by liquid fermentation is mixed with diatomaceous earth at a ratio of 1.2:1 (V / W). The effective amount of live bacteria is 4-5 billion / g.

Embodiment 3

[0034] The process steps are as in Example 1, the rhizosphere soil sample is collected in the separation and cultivation step, 50 grains / 100 grams of soil sample are added to the sclerotia and mixed evenly, the parasitic bacteria are trapped at a constant temperature of 25°C, and taken out after 4 weeks. In the liquid fermentation step, the fermentation culture uses glucose and urea as carbon and nitrogen sources respectively, and the C / N ratio is 15:1. In the liquid fermentation step, the culture in the Erlenmeyer flask was added to the fermentation broth at a ratio of 8%. In the preparation step of the microbial agent, the bacterial slurry obtained from liquid fermentation is mixed with diatomaceous earth at a ratio of 1.5:1 (V / W). The effective amount of live bacteria is 3-4 billion / gram.

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Abstract

A preparing method of a hyperparasitic fungal inoculant for sclerotinia sclerotiorum is disclosed. The method includes following three steps: a step of separation and culturing, a step of liquid fermentation and a step of inoculant preparation. A hyperparasitic fungus having antagonistic effects for the sclerotinia sclerotiorum is screened, separated from soil and cultured. The effective number of living bacteria of the hyperparasitic fungus for the sclerotinia sclerotiorum is increased by changing a sclerotium adding amount, culturing time, a liquid fermentation carbon nitrogen ratio, a fermentation broth inoculation concentration, and a ratio of a fungus liquid to diatomite. A specific antagonistic microbe is screened and cultured, and the fungal inoculant is prepared to control sclerotinia sclerotiorum disease. The hyperparasitic fungus in the fungal inoculant is high in activity. The fungal inoculant can overcome a plurality of disadvantages of chemical pesticides, namely poor control effects, high cost, environment pollution, influences on food safety, and the like.

Description

technical field [0001] The invention relates to a method for preparing a fungal inoculum, in particular to a method for preparing a hyperparasitic fungus inoculum of Sclerotinia sclerotiorum. Background technique [0002] Sclerotinia sclerotiorum is a worldwide important plant pathogen that harms crops and vegetables. It can infect a variety of crops, fruit trees, vegetables and commercial crops, especially causing serious losses in yield and quality of rapeseed, soybeans, sunflowers, peanuts, celery and cucumbers. This pathogen survives the winter and summer in the soil by producing sclerotia, and becomes an important source of primary infection in the next season. At present, the prevention and control of Sclerotinia mainly relies on chemical pesticides. However, chemical control is not only costly, pollutes the environment, but also has unsatisfactory control effects; at the same time, food safety is also seriously affected. A large number of foreign research results ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00
Inventor 胡勤星李世东李延禄彭毅
Owner JIANGSU TIANXIANG BIO TECH
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