Application of fusaric acid to inhibition of ustilaginoidea virens

A technology of fusaric acid and rice smut fungus, which is applied in the application, chemicals for biological control, fungicides, etc., can solve the problem that the inhibitory and control effects of rice smut fungus have not yet been seen, and Jinggangmycin's prevention and control of rice smut The problem of poor effect and singleness can achieve the effect of being conducive to ecological balance and stability, not harming people and animals, and having a long lasting effect.

Active Publication Date: 2014-02-26
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, due to the single use of Jinggangmycin on a large scale all year round, it has been reported that rice smut bacteria are resistant to Jinggangmycin
In 2011, Xiao Youhua found that the relative control effect of 20% Jinggangmycin water-soluble powder on diseased ears was only 40% (test of rice false smut pesticide control, Zunyi Science and Technology, 2011, 2:7-9); and Zhang Min also reported that Jinggang Mymycin has very poor control effect on rice false smut (Causes of large-scale occurrence of rice false smut in Nanchong City and countermeasures, Sichuan Agricultural Science and Technology, 2011, 3: 46-47)
At present, there are commercial products using Fusarium metabolites (biotin) to make fusaric acid in China, but there are no reports on the use of Fusarium metabolites (biotin) to inhibit and control rice smut

Method used

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  • Application of fusaric acid to inhibition of ustilaginoidea virens
  • Application of fusaric acid to inhibition of ustilaginoidea virens
  • Application of fusaric acid to inhibition of ustilaginoidea virens

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Experimental program
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Effect test

Embodiment 1

[0020] 1. Indoor in vitro test

[0021] a) Inhibitory effect of fusaric acid on mycelia growth of different rice smut fungi

[0022] Fusaric acid provided by the present invention was purchased from Tokyo Chemical Industry Co., Ltd.

[0023] According to 5 different concentration gradients (Table 1), fusaric acid was diluted with sterile water, and mixed with potato sucrose medium (PSA medium, 20% potato juice, 2% sucrose, pH 6.0) to make drug-containing Medium.

[0024] Three strains of rice mistletoe from different regions (one in Shanghang County, Fujian Province, one in Nanjing County, and one in Hebei Province) were activated on the PSA medium, and the colonies of the tested bacteria were treated with a puncher with a diameter of 5 mm. Take the bacteria cake from the edge, pick the bacteria cake with an inoculation needle and place it in the middle of the drug-containing medium plate, and cultivate it in the dark at 28 °C for 25 days, then measure the colony diameter of...

Embodiment 2

[0040] 2. Field control experiment

[0041] a) Rice cultivation

[0042] After conventional seed soaking, disinfection and seedling raising, the rice is inserted into a cement pool in a greenhouse with controllable temperature and humidity. The row spacing of rice planting is 17-20 cm, and the conventional cultivation management is carried out. The rice variety is Liangyoupeijiu.

[0043] b) Refer to the EC of fusaric acid indoor in vitro test for Aspergillus oryzae 50 Value, design 3 different concentration gradients. Seven days before the rice was broken, three different concentration gradients of fusaric acid were sprayed on the flag leaves and ear bracts of the rice until dripping, and about 1×10 was artificially injected in the middle of the rice ear bracts the next day. 7 Oryza spp. spore liquid, inject 2 ml per plant. 6 d after inoculation and then applied once again. With water as the control, each treatment was repeated 3 times. The investigation was carried out...

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Abstract

The invention provides application of fusaric acid to inhibition of ustilaginoidea virens. A situation that fusaric acid is capable of effectively inhibiting mycelial growth and spore germination of the ustilaginoidea virens is found through a mycelial growth rate method and a spore germination bacterial colony inhibition method. A situation that the fusaric acid has a better control effect to ustilaginoidea virens is found through a field trial result. Dosages of effective constituents are 200mg / L, 300mg / L and 400mg / L, and average control effects of the effective constituents to the ustilaginoidea virens can respectively reach 48.40 percent, 54.92 percent and 63.08 percent. The first research of the application finds that the fusaric acid has strong inhibition function and good control effect to the ustilaginoidea virens, and provides a new scientific thought for further developing and researching and creating a novel agricultural bactericide and applying to the control of the ustilaginoidea virens.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to the application of fusaric acid in inhibiting rice smut bacteria. Background technique [0002] Rice false smut is caused by Chlorosclerotinum oryzae ( Ustilaginoidea virens (Cooke) Tak) fungal disease of panicles of rice. Because the disease occurs more frequently in the years when rice grows well, it is called "Valley of Harvest Years". The disease occurs in the rice producing areas of the world. Before the 1980s, the disease occurred sporadically and was a secondary disease. After 2000, with the popularization of hybrid rice varieties with dense panicle type, the improvement of fertilization level and the change of climatic conditions, rice false smut has risen from the original secondary disease to the main disease in rice areas. The disease mainly occurs at the heading stage of rice. The rice curve ball produced by the pathogen not only causes a signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/40A01P3/00
Inventor 陈福如甘林杨秀娟阮宏椿杜宜新石妞妞
Owner INST OF PLANT PROTECTION FAAS
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