Application of fusaric acid to inhibition of ustilaginoidea virens
A technology of fusaric acid and rice smut fungus, which is applied in the application, chemicals for biological control, fungicides, etc., can solve the problem that the inhibitory and control effects of rice smut fungus have not yet been seen, and Jinggangmycin's prevention and control of rice smut The problem of poor effect and singleness can achieve the effect of being conducive to ecological balance and stability, not harming people and animals, and having a long lasting effect.
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Embodiment 1
[0020] 1. Indoor in vitro test
[0021] a) Inhibitory effect of fusaric acid on mycelia growth of different rice smut fungi
[0022] Fusaric acid provided by the present invention was purchased from Tokyo Chemical Industry Co., Ltd.
[0023] According to 5 different concentration gradients (Table 1), fusaric acid was diluted with sterile water, and mixed with potato sucrose medium (PSA medium, 20% potato juice, 2% sucrose, pH 6.0) to make drug-containing Medium.
[0024] Three strains of rice mistletoe from different regions (one in Shanghang County, Fujian Province, one in Nanjing County, and one in Hebei Province) were activated on the PSA medium, and the colonies of the tested bacteria were treated with a puncher with a diameter of 5 mm. Take the bacteria cake from the edge, pick the bacteria cake with an inoculation needle and place it in the middle of the drug-containing medium plate, and cultivate it in the dark at 28 °C for 25 days, then measure the colony diameter of...
Embodiment 2
[0040] 2. Field control experiment
[0041] a) Rice cultivation
[0042] After conventional seed soaking, disinfection and seedling raising, the rice is inserted into a cement pool in a greenhouse with controllable temperature and humidity. The row spacing of rice planting is 17-20 cm, and the conventional cultivation management is carried out. The rice variety is Liangyoupeijiu.
[0043] b) Refer to the EC of fusaric acid indoor in vitro test for Aspergillus oryzae 50 Value, design 3 different concentration gradients. Seven days before the rice was broken, three different concentration gradients of fusaric acid were sprayed on the flag leaves and ear bracts of the rice until dripping, and about 1×10 was artificially injected in the middle of the rice ear bracts the next day. 7 Oryza spp. spore liquid, inject 2 ml per plant. 6 d after inoculation and then applied once again. With water as the control, each treatment was repeated 3 times. The investigation was carried out...
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