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PopW antibacterial protein and pseudomonas fluorescens mixed biological preparation PopW-PF1

A technology of Pseudomonas fluorescens and biocontrol preparations, applied in biocides, bactericides, biocides, etc., can solve the problems of high cost and narrow antibacterial spectrum

Inactive Publication Date: 2011-02-16
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Technical problem The purpose of the present invention is to provide and develop an antibacterial protein for the defects of the existing biocontrol agents that have a relatively high control effect on common field diseases, such as narrow antibacterial spectrum and high cost, and sometimes only for single diseases. The biocontrol preparation mixed with biocontrol bacteria can control various diseases in the field at the same time, with a control effect of more than 50%, which is significantly higher than that of antibacterial protein and biocontrol bacteria alone, and has the function of increasing production

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  • PopW antibacterial protein and pseudomonas fluorescens mixed biological preparation PopW-PF1
  • PopW antibacterial protein and pseudomonas fluorescens mixed biological preparation PopW-PF1
  • PopW antibacterial protein and pseudomonas fluorescens mixed biological preparation PopW-PF1

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Embodiment Construction

[0016] The method that biocontrol strains are used to prepare biocontrol agents is the conventional bacterial culture method:

[0017] 1) Inoculate the Pseudomonas fluorescens 4AT8 into the KB culture medium and culture them at 28°C and 180rpm respectively. After 16 to 20 hours, take samples and measure the OD value at 600nm in the ultra-clean workbench. The culture medium is used for zero adjustment; the formula of KB used is King's B: peptone 20g / L, K 2 HPO 4 1.5g / L, MgSO 4 .7H 2O 1.5g / L, add water to dissolve, finally adjust the volume to 1000mL, adjust the pH to 7.0-7.2, and autoclave after subpackaging;

[0018] 2) When the OD value is between 0.5 and 0.8, add the seed bacteria solution to the KB culture solution at a volume ratio of 1:100, shake and cultivate at 180 rpm at 28°C for 20 to 24 hours, and the total concentration of viable bacteria in the finished product is 1× 10 9 CFU / ml.

[0019] The method for PopW antibacterial protein preparation is:

[0020] Th...

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Abstract

The invention relates to a PopW antibacterial protein and biological bactericide mixed biological preparation PopW-PF1, belonging to the technical field of plant protection. The total concentration of viable bacteria in a biological bactericide prepared from pseudomonas fluorescens is 1*10<9>CFU / ml; PopW antibacterial protein has the concentration of 30mg / ml and is diluted into 300-30000 times for use; and in the mixed biological preparation PopW-PF1, the PopW antibacterial protein and the biological bactericide have mixed volume ratio of (30000-30):1, and diluted into 50-100 times when in use. The mixed biological preparation PopW-PF1 has control effect reaching above 50 percent on viral diseases on vegetable crops of pepper, tomato and the like and grain crops of rice, bean and the like, and better control effect on other diseases on the ground such as leaf mold and ustilaginoidea virens, wherein the increase production effect reaches 10 percent.

Description

1. Technical field [0001] The invention relates to PopW-PF1, a mixed biocontrol preparation of PopW antibacterial protein and Pseudomonas fluorescens, which can improve the disease resistance of crops, a kind of antibacterial protein PopW from Ralstonia solanacearum ZJ3721 and a biocontrol bacterium for preventing and controlling plant diseases Pseudomonas fluorescens belongs to the field of biological control and is specially used for the pollution-free control of various vegetables and food crops. 2. Background technology [0002] There are now more than 850 species of plant viruses (including viroids) known in the world, including 1 order, 10 families, 47 genera. Plant virus disease is a very serious plant disease, and it is very difficult to prevent and control. It is known as "plant cancer". The outbreak of plant virus disease is very widespread and the disaster is very serious. Almost all vegetables, food crops, fruit trees, etc. are infected with viral diseases, whi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01P3/00A01N63/02C12R1/39C12P21/00A01P1/00C12N1/20
Inventor 郭坚华刘红霞曹静
Owner NANJING AGRICULTURAL UNIVERSITY
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