Delftia lacustris and application thereof in rice false smut prevention and control

Abstract

The invention discloses delftia lacustris and application thereof in rice false smut prevention and control. The bacterial strain is named as delftia lacustris ZJU-B1 with the preservation number being CCTCC NO: M 2017641 and the preservation date being October 30, 2017. According to the delftia lacustris ZJU-B1 obtained through separation provided by the invention, the occurrence of ustilaginoidea virens can be effectively prevented and controlled, the disease occurrence rate is reduced, the yield and the safety of rice are remarkably improved, and the healthy and pollution-free production ofthe rice is realized.

Description

technical field

[0001] The invention relates to the technical field of crop disease prevention and control, in particular to Delftia chinensis and its application in the prevention and treatment of rice false smut. Background technique

[0002] Rice is the grain crop with the largest planting area and the highest yield, and more than 60% of the population in the country feeds on rice. In the process of rice production, disease is the main factor restricting the yield and quality of rice, often causing huge economic losses.

[0003] At present, rice false smut has been widely distributed in more than 40 countries in the world. Before the 1980s, the disease was a minor disease of rice. With the popularization of rice planting and the improvement of fertilization level, its damage area has been expanding continuously, and it has jumped to become the most important disease of rice in many places. During the curve ball formation process, the nutrition of the diseased ear and th...

Examples

Embodiment 1

[0033] Isolation and identification of embodiment 1 pond-living Delftia

[0034] 1. Isolation of endophytes from rice ears

[0035] Pick healthy rice grains from the diseased strains of rice koji in Wenling, disinfect them with 75% ethanol for about 1 minute, rinse them with sterilized water for 3 times to remove the residual disinfectant, and finally put them on sterile absorbent paper to absorb excess Then put it into a sterile mortar, add 6mL of BPS buffer to grind, take the supernatant and dilute it 10, 50, 100 times, take 200 μL of each dilution and spread it on the LB plate, and verify the last washing water at the same time Spread on LB plates, check whether the tissue surface is thoroughly disinfected, culture in dark at 30°C for 1-2 days, and record the colony shape, size and color.

[0036] The biological characteristics of the above-mentioned Delftia chinensis are: the colony isolated and purified on LB medium is round, with smooth surface, neat edges, shiny, opaqu...

Embodiment 2

[0057] Preparation and application of embodiment 2 biocontrol agents

[0058] 1. Preparation of biocontrol agents

[0059] Streak Delftia chinensis on the LB plate, pick a single colony into a conical flask filled with 50mL LB liquid medium, shake and culture at 180rpm at 30°C for about 18-22h, when the OD value at 600nm is measured When it is between 0.8-1.0, then add the seed solution to a 250mL Erlenmeyer flask with 100mL LB medium at an inoculum amount of 1%, and vibrate at 180rpm at 30°C for 24 hours, and the number of viable bacteria can reach 10 9 CFU / mL.

[0060] In terms of volume fraction, take 87.5% of the prepared fermentation broth, add 2% of dispersant SP-SC29, 33% of dispersant SP-SC, 0.5% of penetrant organosilicon, and 7% of bacterial liquid supernatant. After mixing, the raw Antibiotics, for field use.

[0061] 2. Effect of field application of biocontrol agents on rice false smut

[0062] Application location: Wenling Time: 5-7 days before the rice break...

technical field

[0001] The invention relates to the technical field of crop disease prevention and control, in particular to Delftia chinensis and its application in the prevention and treatment of rice false smut. Background technique

[0002] Rice is the grain crop with the largest planting area and the highest yield, and more than 60% of the population in the country feeds on rice. In the process of rice production, disease is the main factor restricting the yield and quality of rice, often causing huge economic losses.

[0003] At present, rice false smut has been widely distributed in more than 40 countries in the world. Before the 1980s, the disease was a minor disease of rice. With the popularization of rice planting and the improvement of fertilization level, its damage area has been expanding continuously, and it has jumped to become the most important disease of rice in many places. During the curve ball formation process, the nutrition of the diseased ear and th...

Examples

Embodiment 1

[0033] Isolation and identification of embodiment 1 pond-living Delftia

[0034] 1. Isolation of endophytes from rice ears

[0035] Pick healthy rice grains from the diseased strains of rice koji in Wenling, disinfect them with 75% ethanol for about 1 minute, rinse them with sterilized water for 3 times to remove the residual disinfectant, and finally put them on sterile absorbent paper to absorb excess Then put it into a sterile mortar, add 6mL of BPS buffer to grind, take the supernatant and dilute it 10, 50, 100 times, take 200 μL of each dilution and spread it on the LB plate, and verify the last washing water at the same time Spread on LB plates, check whether the tissue surface is thoroughly disinfected, culture in dark at 30°C for 1-2 days, and record the colony shape, size and color.

[0036] The biological characteristics of the above-mentioned Delftia chinensis are: the colony isolated and purified on LB medium is round, with smooth surface, neat edges, shiny, opaqu...

Embodiment 2

[0057] Preparation and application of embodiment 2 biocontrol agents

[0058] 1. Preparation of biocontrol agents

[0059] Streak Delftia chinensis on the LB plate, pick a single colony into a conical flask filled with 50mL LB liquid medium, shake and culture at 180rpm at 30°C for about 18-22h, when the OD value at 600nm is measured When it is between 0.8-1.0, then add the seed solution to a 250mL Erlenmeyer flask with 100mL LB medium at an inoculum amount of 1%, and vibrate at 180rpm at 30°C for 24 hours, and the number of viable bacteria can reach 10 9 CFU / mL.

[0060] In terms of volume fraction, take 87.5% of the prepared fermentation broth, add 2% of dispersant SP-SC29, 33% of dispersant SP-SC, 0.5% of penetrant organosilicon, and 7% of bacterial liquid supernatant. After mixing, the raw Antibiotics, for field use.

[0061] 2. Effect of field application of biocontrol agents on rice false smut

[0062] Application location: Wenling Time: 5-7 days before the rice break...

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