Lysinibacillus fusiformis H16 and application thereof
A lysinic bacillus, spindle-shaped technology, applied in the field of microorganisms, can solve the problems of harm, reduction of rice yield and quality, seed setting rate and thousand-grain weight reduction, etc.
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Embodiment 1
[0018] Screening and Identification of Example 1 Bacterial Strain H16
[0019] Multiple bacterial strains obtained from the soil of the China Rice Research Institute through enrichment culture, isolation and purification were carried out for the antagonism experiment of rice false smut, and a bacterial strain was successfully screened through the antagonism experiment, which was named strain H16. Colony morphology such as figure 1 shown. The colonies of the strain H16 are round, smooth, off-white, flat, transparent, 1-2mm in diameter, with neat edges.
[0020] Strain H16 was identified as a Lysinibacillus fusiformis strain by 16s sequencing and comparative analysis, and the phylogenetic tree is as follows: figure 2 shown.
Embodiment 2
[0021] Embodiment 2 Antagonism test
[0022] (1) Strain culture
[0023] A, the cultivation of rice smut bacteria strain YY7850:
[0024] Inoculate the rice smut strain YY7850 preserved in our laboratory onto a potato sucrose agar medium (PSA) plate, cultivate at a constant temperature of 28°C, and inoculate it into a potato sucrose liquid medium (PS) after the colony grows, 150rpm, 28°C Cultivate at a constant temperature for 7 days, filter through eight layers of gauze to obtain a spore suspension, and set aside.
[0025] Potato sucrose agar medium (PSA): The medium adopts the PSA agar medium of Beijing Suo Laibao Technology Co., Ltd., and the PSA agar medium is prepared according to the amount of 47.0 g of PSA agar medium per liter of water. Sterilize with damp heat for 20 minutes, take out and shake well, cool and store at 4°C for later use.
[0026] Potato sucrose liquid medium (PS): Add 200g of potatoes to 1000mL of water and boil for 20-30min, filter through eight la...
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