Lysinibacillus fusiformis H16 and application thereof

A lysinic bacillus, spindle-shaped technology, applied in the field of microorganisms, can solve the problems of harm, reduction of rice yield and quality, seed setting rate and thousand-grain weight reduction, etc.

Inactive Publication Date: 2021-06-25
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the reproduction process of rice false smut bacteria, a large amount of nutrients are consumed in diseased grains, which not only harms the grain itself, but also affects the nutrient transport of adjacent grains, resulting in an increase in empty grain rate, a decrease in seed setting rate and 1,000-grain weight, resulting in a reduction in yield
Rice smut not only forms rice bent balls on the ears of rice, which seriously affects the growth of grains, reduces the yield and quality of rice, and the rice smut toxin produced by the pathogenic fungus Chlorosclerotinum oryzae is also toxic to humans and animals.

Method used

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  • Lysinibacillus fusiformis H16 and application thereof
  • Lysinibacillus fusiformis H16 and application thereof
  • Lysinibacillus fusiformis H16 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Screening and Identification of Example 1 Bacterial Strain H16

[0019] Multiple bacterial strains obtained from the soil of the China Rice Research Institute through enrichment culture, isolation and purification were carried out for the antagonism experiment of rice false smut, and a bacterial strain was successfully screened through the antagonism experiment, which was named strain H16. Colony morphology such as figure 1 shown. The colonies of the strain H16 are round, smooth, off-white, flat, transparent, 1-2mm in diameter, with neat edges.

[0020] Strain H16 was identified as a Lysinibacillus fusiformis strain by 16s sequencing and comparative analysis, and the phylogenetic tree is as follows: figure 2 shown.

Embodiment 2

[0021] Embodiment 2 Antagonism test

[0022] (1) Strain culture

[0023] A, the cultivation of rice smut bacteria strain YY7850:

[0024] Inoculate the rice smut strain YY7850 preserved in our laboratory onto a potato sucrose agar medium (PSA) plate, cultivate at a constant temperature of 28°C, and inoculate it into a potato sucrose liquid medium (PS) after the colony grows, 150rpm, 28°C Cultivate at a constant temperature for 7 days, filter through eight layers of gauze to obtain a spore suspension, and set aside.

[0025] Potato sucrose agar medium (PSA): The medium adopts the PSA agar medium of Beijing Suo Laibao Technology Co., Ltd., and the PSA agar medium is prepared according to the amount of 47.0 g of PSA agar medium per liter of water. Sterilize with damp heat for 20 minutes, take out and shake well, cool and store at 4°C for later use.

[0026] Potato sucrose liquid medium (PS): Add 200g of potatoes to 1000mL of water and boil for 20-30min, filter through eight la...

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Abstract

The invention discloses lysinibacillus fusiformis H16 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Lysinibacillus fusiformis H16, and the preservation number of the Lysinibacillus fusiformis H16 is CCTCC (China Center for Type Culture Collection) NO: M 2021179. The Lysinibacillus fusiformis H16 disclosed by the invention can inhibit the growth of the Ustilaginoidea virens strain YY7850, which is an example that the Lysinibacillus fusiformis is found to have antagonism to the Ustilaginoidea virens pathogenic bacteria for the first time, and due to the fact that the strain H16 has the capacity of inhibiting the Ustilaginoidea virens pathogenic bacteria, the strain has the potential of being developed into a rice false smut biocontrol bacterium.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a spindle-shaped lysine bacillus H16 and its application. Background technique [0002] Rice false smut is a fungal disease on rice spikes caused by the infection of the rice false smut fungus - Ustilaginodea virens (Cooke) Takahashi. It infects glumes, glumes, and grains in the late growth stage of rice, commonly known as "harvest disease". . Rice false smut is a worldwide rice disease, which occurs in many countries such as China, Japan, the United States, India, and the Philippines. In recent years, it has been upgraded from a minor disease to one of the major diseases on rice. The smut fungus consumes a large amount of nutrients in the diseased grains during the reproduction process, which not only harms the grain itself, but also affects the nutrient transport of adjacent grains, resulting in an increase in the empty grain rate, a decrease in the seed setting rate a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/20
Inventor 林晓燕陈铭学曹赵云杨欢
Owner CHINA NAT RICE RES INST
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