Environment-friendly biological agent for repairing and treating petroleum polluted water and soil and preparation method of environment-friendly biological agent

Abstract

The invention belongs to the technical field of environment, and particularly relates to an environment-friendly biological agent for repairing and treating petroleum polluted water and soil and a preparation method of the environment-friendly biological agent. The biological agent is prepared by mixing a compound microbial agent, a biological surfactant and an auxiliary material in parts by mass, has the characteristics of high efficiency, environment friendliness and no secondary pollution, can remarkably improve the remediation efficiency of petroleum-contaminated soil, and has a wide application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of environmental technology, and particularly relates to an environment-friendly biological agent for the remediation of oil-polluted water bodies and soil and a preparation method thereof. Background Art

[0002] Oil is the "blood" of modern industry. However, with the increasing amount of oil exploitation, during exploitation, transportation, storage, and accidental leakage, a large amount of oil enters the environment, seriously polluting soil, groundwater, rivers, and the ocean. Especially in recent decades, oil has become one of the main pollutants in the environment such as soil and water bodies. Among the remediation methods for oil pollution, microbial remediation is a new technology with good treatment effects, low cost, strong adaptability, no secondary pollution, etc., and is a clean environment-friendly technology with low investment, high benefits, easy application, and great development potential.

[0003] Different microorganisms have different degradation abilities for different components, and the degradation ability of a single strain is limited, making it difficult to cope with the complex and changeable oil-polluted environment or play different roles in degradation. Therefore, inoculating a mixed microbial strain may have a better effect on oil pollution control. Therefore, developing efficient and stable composite microbial agents is of great significance for improving the efficiency of oil-polluted soil remediation. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide an environment-friendly biological agent for the remediation of oil-polluted water bodies and soil and a preparation method thereof. By using biosurfactants to improve the dispersibility and biodegradability of oil pollutants, creating a favorable environment for microbial cells to contact and degrade petroleum hydrocarbons, and then degrading the pollutants in the polluted water bodies and soil through a microbial flora, the purpose of treating and remediating oil-polluted water bodies and soil is achieved.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is: an environment-friendly biological agent for the remediation of oil-polluted water bodies and soil, which is prepared by mixing 30 - 50 parts by mass of a composite microbial agent, 10 - 30 parts by mass of a biosurfactant, and 20 - 40 parts by mass of auxiliary materials.

[0006] The composite microbial agent is prepared by compounding Bacillus subtilis, Acinetobacter sp., Aspergillus niger, and Pseudomonas sp. according to the proportion of bacterial content. Among them, the effective viable count of Bacillus subtilis ≥ 2.0×10 8 cfu / g, the effective viable count of Acinetobacter sp. ≥ 2.0×10 8 cfu / g, the effective spore count of Aspergillus niger ≥ 2.0×10 8 cfu / g, the effective viable count of Pseudomonas sp. ≥ 2.0×10 8cfu / g.

[0007] Further, the deposit information of the Bacillus subtilis strain used is: deposit classification name : Bacillus subtilis Bacillus subtilis, accession no. : CGMCC No. 5550 , deposit date : December 8, 2011 , preservation unit : China : China General Microbiological Culture Collection Center , address of depository : Yard 1, Beichen West Road, Chaoyang District, Beijing No. 3 .

[0008] The biosurfactant is one or a mixture of two or more of sophorolipids, rhamnolipids, and other biosurfactants.

[0009] The auxiliary material is one or a mixture of two or more of attapulgite, diatomaceous earth, bentonite and zeolite powder.

[0010] The preparation method of the present invention comprises the following steps: S1: Bacillus subtilis, Acinetobacter, Aspergillus niger and Pseudomonas are cultured to the logarithmic growth phase respectively, and then mixed in proportion to obtain a composite microbial agent; S2: uniformly mixing the biosurfactant and the excipient to obtain a mixture; S3: Fully mix the composite microbial agent with the mixture to obtain the biological agent. Beneficial Effects

[0011] 1. High degradation efficiency: The various microorganisms in the composite microbial agent can work synergistically to efficiently degrade petroleum pollutants.

[0012] 2. Environmentally friendly: The microorganisms and biosurfactants in biological preparations are natural substances and will not cause harm to the environment and human body.

[0013] 3. Strong adaptability: Biological agents can function under different environmental conditions and are suitable for the restoration and treatment of various petroleum-contaminated water bodies and soils.

[0014] 4. Good stability: The microorganisms and excipients in biological preparations can promote each other and maintain good stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 These are the steps for preparing the biological preparation of the present invention. DETAILED DESCRIPTION

[0016] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments and experimental examples. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0017] Please refer to the attached Figure 1 The preferred embodiment of the present invention is described below. Figure 1 The schematic diagram of the preparation steps is simplified, and only the basic steps of the present invention are illustrated schematically, so it only shows the processes related to the present invention. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the scope of protection of the present invention. Example

[0018] This embodiment is to achieve the preparation of environmentally friendly biological preparations as follows: Bacillus subtilis, acinetobacter, Aspergillus niger and Pseudomonas are cultured to the logarithmic growth phase respectively, and then mixed in proportion to obtain a composite microbial agent, wherein the effective viable count of Bacillus subtilis is ≥2.0×10 8 cfu / g, effective viable count of Acinetobacter ≥2.0×10 8 cfu / g, effective spore count of Aspergillus niger ≥ 2.0×10 8 cfu / g, effective viable count of Pseudomonas ≥ 2.0×10 8 cfu / g.

[0019] Prepare the raw materials according to the following mass parts: 40 parts of composite microorganisms, 15 parts of sophorolipids, 10 parts of rhamnolipids, and 35 parts of attapulgite.

[0020] The sophorolipids and rhamnolipids are uniformly mixed with glucose and attapulgite to obtain a mixture.

[0021] The composite microbial agent is fully mixed with the mixture to obtain the biological agent. Example

[0022] Prepare the raw materials according to the following mass parts: 45 parts of composite microorganisms, 15 parts of sophorolipids, 5 parts of betaine, and 35 parts of bentonite.

[0023] The preparation method is the same as Example 1.

[0024] Experimental comparison: Example 1 and Example 2 were respectively applied to the restoration and treatment of a certain petroleum-contaminated soil. So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. An environment-friendly biological agent for the remediation of oil-polluted water bodies and soil, characterized in that, The invention is prepared by mixing the following components in parts by weight: 30-50 parts of composite microbial agent, 10-30 parts of biological surfactant and 20-40 parts of auxiliary materials.

2. The environment-friendly biological agent according to claim 1, characterized in that, The composite microbial inoculum is prepared by compounding Bacillus subtilis, Acinetobacter, Aspergillus niger and Pseudomonas according to the proportion of bacterial content, wherein the effective viable count of Bacillus subtilis ≥ 2.0×10 8 cfu / g, the effective viable count of Acinetobacter ≥ 2.0×10 8 cfu / g, the effective spore count of Aspergillus niger ≥ 2.0×10 8 cfu / g, the effective viable count of Pseudomonas ≥ 1.0×10 8 cfu / g.

3. The environment-friendly biological agent according to claim 1, characterized in that, The biosurfactant is one or a mixture of two or more of sophorolipids, rhamnolipids, and other biosurfactants.

4. The environment-friendly biological agent according to claim 1, wherein The auxiliary material includes one or a mixture of two or more of attapulgite, diatomaceous earth, bentonite and zeolite powder.

5. A method for preparing the environmentally friendly biological agent according to claim 1, comprising the following steps: S1: Bacillus subtilis, Acinetobacter, Aspergillus niger and Pseudomonas are cultured to the logarithmic growth phase respectively, and then mixed in proportion to obtain a composite microbial agent; S2: uniformly mixing the biosurfactant and the excipient to obtain a mixture; S3: Fully mix the composite microbial agent with the mixture to obtain the biological agent.

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