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Method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri

A technology of copper algae powder and fucoxanthin, which is applied in the field of marine biological natural products, can solve the problems of high energy consumption and low purity, and achieve the effects of low energy consumption, low degradation rate and low harvesting cost

Active Publication Date: 2018-09-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN105859657A discloses a method for extracting crude fucoxanthin from copper algae. The patent extracts crude fucoxanthin from copper algae through pretreatment, suction filtration, ultrasonic leaching, and extraction. The pretreatment involves algae Freeze-drying of the body, although the activity of fucoxanthin is maintained to a certain extent, the energy consumption of this process is high. In addition, the crude product of fucoxanthin is extracted by this method without separation, and the purity is low

Method used

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  • Method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri
  • Method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri
  • Method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri

Examples

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Embodiment 1

[0050] Using manual salvage, select the time period with weak ambient light (early morning or evening) to harvest fresh copper algae, and use shade cloth to cover the harvested algae. After harvesting, transfer the copper algae to a cool workshop with an ambient light of about 180 Lux, and immediately rinse and soak the fresh copper algae with clean seawater. Sand, small plankton, hook shrimp and other organisms. Use clean fresh water at a temperature of around 20°C to quickly rinse the copper algae for 1 minute. Spread the cleaned copper algae on the shelf, manually remove the algae, drain the water and dry in the shade for 12 hours.

[0051] Transfer the semi-shade-dried copper algae that has been fully drained to an electric oven, and dry at 55°C for 20 hours, turning it every 1 hour, drying continuously for 12 hours, and blowing nitrogen at 20L / min. The dried copper algae was frozen and ground into powder with liquid nitrogen, and passed through a 65-mesh sieve. Irradia...

Embodiment 2

[0055] Harvest fresh copper algae from the cultivation sea area, and use shade cloth to cover the harvested algae. Transfer the copper algae to a cool workshop with an ambient light of about 180Lux, immediately wash and soak the fresh copper algae with clean seawater, the seawater temperature is about 20°C, and the salinity is 30‰, fully remove the silt, small Plankton, hook shrimp and other organisms. Use clean fresh water at a temperature of around 20°C to quickly rinse the copper algae for 1 minute. Spread the cleaned copper algae flat on the shelf, manually remove the algae, drain the water and dry in the shade for 12 hours.

[0056] Transfer the semi-shade-dried copper algae that has been fully drained to an electric oven, dry at 60°C for 24 hours, turn it every 1 hour, and blow nitrogen at 20L / min. The dried copper algae was frozen and ground into powder with liquid nitrogen, and passed through a 90-mesh sieve. Irradiation treatment of algae powder. Weigh 2g of algae...

Embodiment 3

[0060] Harvest fresh copper algae from the cultivation sea area, and use shade cloth to cover the harvested algae. Transfer the copper algae to a cool workshop with an ambient light of about 100Lux, immediately wash and soak fresh copper algae with clean seawater, the seawater temperature is about 20°C, and the salinity is 30‰, fully remove the sediment, small Plankton, hook shrimp and other organisms. Use clean fresh water at a temperature of around 20°C to quickly rinse the copper algae for 1 minute. Spread the cleaned copper algae flat on the shelf, manually remove the algae, drain the water and dry in the shade for 12 hours.

[0061] Transfer the semi-shade-dried copper algae that has been fully drained to an electric oven, dry at 60°C for 24 hours, turn it every 1 hour, and blow nitrogen at 20L / min. The dried copper algae was frozen and ground into powder with liquid nitrogen, and passed through a 90-mesh sieve. Irradiation treatment of algae powder. Weigh 2g of algae...

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Abstract

The invention belongs to the technical field of marine organism natural products and particularly relates to a method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri. The method includes steps: preparing sargassum horneri powder by cleaning, drying in the shade, drying, powdering, sterilizing and the like; adding extract liquid and steel balls into the sargassum horneri powder, performing oscillatory extraction for 3-10 minutes under weak-light, low-temperature and oxygen-free conditions, centrifuging to obtain supernatant, and subjecting the supernatant to high performance liquid chromatography and vacuum freeze drying to obtain a pure fucoxanthin product. The method has advantages of high efficiency, simplicity, low cost and the like, provides basis for biological resource comprehensive utilization of sargassum horneri and has a potential significant economic value.

Description

technical field [0001] The invention belongs to the technical field of marine biological natural products, and in particular relates to the preparation of copper algae powder and a method for extracting fucoxanthin in the powder. Background technique [0002] Copper algae (Sargassum horneri) is a large floating brown algae that is widely distributed in the northern seas of my country. It has the characteristics of wide adaptability, strong stress resistance, and easy cultivation. Economic brown algae. [0003] Fucoxanthin (also known as fucoxanthin) is a carotenoid substance composed of three elements: carbon, hydrogen, and oxygen, and is widely distributed in some diatoms and large brown algae. Due to the many conjugated double bonds in its chemical structure, it has antioxidant properties and can effectively remove free radicals in organisms. Fucoxanthin can promote the energy consumption of adipocytes by inducing the expression of uncoupling protein 1 in the mitochondria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 王广策顾文辉何林文解修俊王旭雷牛建峰郇丽
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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