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Alkaloid compound as well as preparation method and application thereof

A technology for compounds and alkaloids, applied in the field of alkaloid compounds and their preparation, can solve the problem of seldom separation of alkaloid compounds, and achieve the effects of good development prospects, high extraction yield and high product purity

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, alkaloid compounds are rarely isolated from this genus

Method used

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  • Alkaloid compound as well as preparation method and application thereof
  • Alkaloid compound as well as preparation method and application thereof
  • Alkaloid compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 isolates fungi from copper algae

[0034]Copper algae (Sargassum horneri) were collected from the coast of Zhejiang. After the samples were brought back to the laboratory, they were first rinsed with sterile seawater 3 times to remove non-attached microorganisms; the copper algae were placed in a centrifuge tube, then a small amount of seawater was added, and the samples were washed with a vortex shaker. Shake for 10 minutes, then centrifuge the suspension after removing the algae for 20 minutes (5000r / min), pour off the supernatant; suspend the sediment with a small amount of sterile seawater, take 0.1mL and spread it on Martin's medium (containing gentamicin 8 U / L) plate; after culturing for 10 days at room temperature at 20°C, pick a single colony, streak and purify it, and move it to a slant for storage at 4°C for later use.

Embodiment 2

[0035] The identification of embodiment 2 Discochaete sp. fungus Z233

[0036] When the isolated fungus was cultured on PDA, the mycelium was fluffy and soft, and it was ring-like in the early stage. After culturing for 8 days, conidia discs were produced on the mycelium, which were distributed throughout the colony; conidia discs were spherical, with ink-like mucus at the top, that is, the conidia produced; Shaped, with 5 cells, the middle 3 cells are colored, without obvious constriction at the septum; the apical cells are short and colorless, and the basal cells are smaller; there are 3 flagella.

[0037] At the same time, the ITS gene sequence of the strain is determined, and the ITS sequence of the strain is shown as SEQ ID No.1 in the sequence list.

[0038] According to the morphological characteristics of the strain and the results of ITS sequence analysis, the strain was identified as Pestalotiopsis sp. and named as Pestalotiopsis sp. Z233.

Embodiment 3

[0039] The fermentative culture of embodiment 3 Pseudomonas fungus Z233

[0040] The activated spore suspension of the fungus Z233 of the genus Discotrichum genus Z233 was made into a spore suspension, then inoculated into the culture medium, and cultured for static fermentation at 25° C. for 20 days.

[0041] Among them, the formula of the culture medium is: starch 3g, bran 14g, yeast extract 6g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.4g, sea water 1000mL.

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PUM

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Abstract

The invention discloses an alkaloid compound as well as a preparation method and an application of the alkaloid compound. The alkaloid compound has a molecular formula of C15H21NO4. The preparation method of the alkaloid compound comprises the steps that activated Pestalotiopsis sp. fungi Z233 are inoculated into culture liquid, and the fermentation culture is carried out for 10 to 40 days at 20 to 30 DEG C; mycelia and fermentation liquid are obtained through separation after the fermentation culture; after the mycelia are leached, extracted and concentrated by organic solvents, mycelium extractives are obtained; or the fermentation liquid is extracted and concentrated to obtain fermentation liquid extractives; and the chromatographic separation and the purification treatment are carried out on the mycelium extractives or the fermentation liquid extractives, and the alkaloid compound is prepared. The alkaloid compound with a novel structure is extracted and separated from fermentation culture substances of Sargassum horneri epiphytic Pestalotiopsis sp. fungi Z233, and the alkaloid compound has better antitumor activity, can be used for preparing antitumor medicine and tumor prevention health-care food and has good development prospects.

Description

technical field [0001] The invention relates to the field of marine active ingredients, in particular to an alkaloid compound and its preparation method and application. Background technique [0002] Copper algae (Sargassum horneri) is a large seaweed of the genus Sargassum, with tall strains and luxuriant branches and leaves. It is a dominant species of vegetation in warm temperate seas and shallow seas in China. The chemical composition of copper algae has been studied to some extent, mainly including 2,4-hydroxy-2,4-vinyl-cholesterol, fucosterol, 5α,8α-peroxyergosta-6,2,2-diene-3β -alcohol, β-sitosterol, etc. (Yuan Qingxiang, Fu Ling, Study on the Chemical Constituents of Sargassu Horneri, Guangdong Chemical Industry, Volume 33, Issue 5, 2006). [0003] There are many studies on the chemical constituents of Pestalotiopsis. Domestic scholars such as Che Yongsheng conducted a systematic study on the chemical components of this genus. In 2011, pestaloquinols A and B were i...

Claims

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Application Information

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IPC IPC(8): C07C229/36C12P13/00A61K31/185A61P35/00A23L1/30C12R1/645
Inventor 吴斌
Owner ZHEJIANG UNIV
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