Sargassum horneri beta-sitosterol compound and extracting method and application thereof

A compound, sitosterol technology, applied in the direction of steroids, organic chemistry, drug combination, etc., can solve the problems of limited long-term use, uncomfortable side effects, etc., and achieve the effects of low equipment requirements, simple preparation methods, and simple process steps

Inactive Publication Date: 2016-11-23
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, many drugs are available for antidepressant in clinical application, such as amitepine, clomipramine, moclobemide, fluoxetine, paroxetine, sertraline, citalopram, etc., a

Method used

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  • Sargassum horneri beta-sitosterol compound and extracting method and application thereof
  • Sargassum horneri beta-sitosterol compound and extracting method and application thereof
  • Sargassum horneri beta-sitosterol compound and extracting method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A copper algae β-sitosterol compound extraction method, the specific steps are: take dried copper algae, soak in 95% ethanol for 2.5 hours after crushing, and filter; the extract is concentrated under reduced pressure and desalted to obtain a crude extract; crude Suspend the extract with distilled water, and extract it with petroleum ether, ethyl acetate, and n-butanol for 4 times in sequence to obtain a partial extract of petroleum ether, which is extracted and chromatographed on a 380-mesh silica gel column, and the gradient of petroleum ether-acetone is repeatedly eluted , purified by Sephasex LH-20 to obtain white solid substances (petroleum ether: acetone = 15:1), the structure of the obtained compound was determined by IR, 1H-NMR, 13C-NMR, MS, melting point: Mp. 136.7-138.4 °C; IR (KBr)cm -1 : 3397, 2932, 1644, 1470, 1341, 1050; 1 H-NMR (CDCl 3, 500 MHz ): δ 0.78 (3H, s, H-18), 1.02 (3H, d, H-21), 1. 08 (3H, s, H-19), 1.61 (3H, t, H-29), 2. 18( 1H, m, H-2...

Embodiment 2

[0027] A copper algae β-sitosterol compound extraction method, the specific steps are: take the dried copper algae, soak in 95% ethanol for 2 hours after crushing, and filter; the extract is concentrated under reduced pressure and desalted to obtain a crude extract; crude extract The substance was suspended in distilled water, and extracted four times with petroleum ether, ethyl acetate, and n-butanol successively to obtain a partial extract of petroleum ether, which was extracted and chromatographed on a 300-mesh silica gel column, and the petroleum ether-acetone gradient was repeatedly eluted. Sephasex LH-20 was purified to obtain white solid substances (petroleum ether: acetone = 15:1). The structures of the obtained compounds were determined by IR, 1H-NMR, 13C-NMR, and MS. Melting point: Mp. 136.7-138.4 °C; IR (KBr)cm -1 : 3397, 2932, 1644, 1470, 1341, 1050; 1 H-NMR (CDCl 3, 500 MHz ): δ 0.78 (3H, s, H-18), 1.02 (3H, d, H-21), 1. 08 (3H, s, H-19), 1.61 (3H, t, H-29...

Embodiment 3

[0031] A method for extracting copper algae β-sitosterol compounds, the specific steps are: take dried copper algae, soak in 95% ethanol for 3 hours after crushing, and filter; obtain a crude extract after decompression concentration and desalination of the extract; crude extraction The substance was suspended in distilled water, and extracted 4 times with petroleum ether, ethyl acetate, and n-butanol in sequence to obtain a partial extract of petroleum ether, which was extracted, and chromatographed on a 400-mesh silica gel column, and the gradient of petroleum ether-acetone was repeatedly eluted. Sephasex LH-20 was purified to obtain white solid substances (petroleum ether: acetone = 15:1). The structures of the obtained compounds were determined by IR, 1H-NMR, 13C-NMR, and MS. Melting point: Mp. 136.7-138.4 °C; IR (KBr) cm -1 : 3397, 2932, 1644, 1470, 1341, 1050; 1 H-NMR (CDCl 3, 500 MHz ): δ 0.78 (3H, s, H-18), 1.02 (3H, d, H-21), 1. 08 (3H, s, H-19), 1.61 (3H, t, ...

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Abstract

The invention discloses a sargassum horneri beta-sitosterol compound and an extracting method thereof. The sargassum horneri beta-sitosterol compound has remarkable anti-depression effect, and animal experiments in an embodiment show that beta-sitosterol can remarkably reduce dead time of mice in a mouse forced swimming test and a tail suspension test, takes effect quickly, is small in toxic and side effects (TD50 is greater than 2,000 mg/kg), and can be used for a long time, and pharmacological properties of the sargassum horneri beta-sitosterol compound which has high value and serves as anti-depression drug are shown. The beta-sitosterol compound is abundant in source of raw materials and is low in price, the extracting method is simple, and the beta-sitosterol compound can be prepared into anti-depression drugs in various dosage forms.

Description

technical field [0001] The invention relates to a β-sitosterol, in particular to a copper algae β-sitosterol compound and its extraction method and application. Background technique [0002] Depression, also known as depressive disorder, is characterized by marked and persistent low mood as the main clinical feature. It is the main type of mood disorder. Clinically, it can be seen that the low mood is not commensurate with the situation. The depression of mood can range from gloomy to heartbroken, low self-esteem, depression, and even pessimism World-weary, there may be suicide attempts or behaviors, and even stupor. Some cases have obvious anxiety and motor agitation, and severe cases have hallucinations, delusions and other psychotic symptoms. Depression can affect people's digestive, immune and nervous system functions, and even cause losses, and the disease has the characteristics of high incidence, refractory cure and high recurrence rate. [0003] At present, many dru...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61K31/575A61P25/24
CPCC07J9/00
Inventor 关丽萍刘炳禹夏亚男杨立业
Owner ZHEJIANG OCEAN UNIV
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