3 results about "Tail suspension test" patented technology

The present application belongs to the field of biological medicine and food technology, and particularly relates to application of milk-derived extracellular vesicles in improving brain health and / or improving nerve-related discomfort conditions. Compared with the prior art, the present application constructs a chronic restraint stress (CRS) induced anxiety model in adolescent mice, and uses open field test, buried bead test, tail suspension test and other behavioral experiments for functional evaluation. The results show that the milk-derived extracellular vesicles significantly improve the anxiety-like behavior of mice, indicating that the milk-derived extracellular vesicles can significantly improve the anxiety-like behavior of adolescent mice induced by chronic restraint stress (CRS) by remodeling the gut-brain axis function, and that the supplementation of milk-derived extracellular vesicles in adolescents can significantly reduce the susceptibility to anxiety in adulthood. At the same time, the present application also constructs a D-gal induced cognitive impairment model in mice, and the results show that it can reduce the production of pro-inflammatory cytokines, inhibit mucosal inflammation, thereby maintaining intestinal homeostasis and alleviating cognitive impairment.

The application discloses a tail suspension test device and method for evaluating the behavioral despair of mice after stroke, wherein a slope plate is arranged below the hanging piece for hanging the mice in the hanging chamber, so as to prevent the mice from struggling due to movement coordination disorders, to help the suspended mice keep balance, and to prevent the mice from shaking even if the movement ability of the mice is impaired, and the application is particularly suitable for evaluating the despair of the mice with impaired movement coordination after stroke. The application supports part of the body weight of the mice through the slope plate, the pain caused during the test is slight, and the application can also be used for rats once the size of the device is adapted to the rats. The application is a modified detection method (mTST for short) of TST, and is used for evaluating the behavioral despair of the mice after stroke. The application can be applied to the evaluation of the curative effect of antidepressant drugs on the model mice of chronic mild stress (CMS) and post-stroke depression (PSD), and is favorable to the basic research of diseases such as PSD and the clinical transformation of drugs.

The invention belongs to the technical field of biological medicine and food, and particularly relates to application of milk-derived extracellular vesicles to improvement of brain health conditions and / or improvement of nerve-related discomfort conditions. Compared with the prior art, a chronic constraint stress (CRS)-induced puberty mouse anxiety model is constructed, functional evaluation is performed by behavioral experiments such as an open field experiment, a bead embedding experiment and a tail suspension experiment, and results show that the milk-derived extracellular vesicles remarkably improve mouse anxiety-like behaviors; it is shown that the milk-derived extracellular vesicles can significantly improve the chronic bound stress (CRS) induced adolescent mouse anxiety-like behavior by reshaping the intestinal-brain axis function, and the anxiety susceptibility of adolescents can be significantly reduced by supplementing the milk-derived extracellular vesicles to teenagers; meanwhile, a D-gal induced mouse cognitive impairment model is constructed, and the result shows that mucosa inflammation can be inhibited by reducing generation of proinflammatory cytokines, so that intestinal homeostasis is maintained, and cognitive impairment is relieved.