5 results about "Restraint stress" patented technology

The present application belongs to the field of biological medicine and food technology, and particularly relates to application of milk-derived extracellular vesicles in improving brain health and / or improving nerve-related discomfort conditions. Compared with the prior art, the present application constructs a chronic restraint stress (CRS) induced anxiety model in adolescent mice, and uses open field test, buried bead test, tail suspension test and other behavioral experiments for functional evaluation. The results show that the milk-derived extracellular vesicles significantly improve the anxiety-like behavior of mice, indicating that the milk-derived extracellular vesicles can significantly improve the anxiety-like behavior of adolescent mice induced by chronic restraint stress (CRS) by remodeling the gut-brain axis function, and that the supplementation of milk-derived extracellular vesicles in adolescents can significantly reduce the susceptibility to anxiety in adulthood. At the same time, the present application also constructs a D-gal induced cognitive impairment model in mice, and the results show that it can reduce the production of pro-inflammatory cytokines, inhibit mucosal inflammation, thereby maintaining intestinal homeostasis and alleviating cognitive impairment.

This invention belongs to the field of biomedical technology, specifically relating to a DNA tetrahedral framework-based nanocomposite, its preparation method, and its application in improving depression. This invention successfully synthesized the nanocomposite material tFNAs-miR146a by loading miR-146a onto a tetrahedral framework nucleic acid with sticky ends. This nanocomposite material can efficiently cross the blood-brain barrier and has been applied to chronic restraint stress-induced depression-like mice, exhibiting biosafety. It shows a synergistic effect in alleviating depression-like behavior and depression-induced neuroinflammation, demonstrating promising potential in the preparation of drugs for treating depression.

The invention discloses a laboratory mouse model construction method for post-traumatic stress disorder research, which comprises the following steps: step S01, putting a laboratory mouse into a conditional fear test box, and adapting to the environment of the conditional fear test box; s02, applying conditional stimulation to the laboratory mouse for a first preset time, wherein the conditional stimulation comprises sound signal stimulation or constraint stress; before or when the conditional stimulation is finished, electric shock is applied to the laboratory mouse for a second preset time, and the current of the electric shock is 1.2-1.6 mA; and S03, repeating the step S02 for preset times, and obtaining the laboratory mouse model for post-traumatic stress disorder research by taking the time interval between two adjacent repetitions as third preset time.

The invention belongs to the technical field of biological medicine and food, and particularly relates to application of milk-derived extracellular vesicles to improvement of brain health conditions and / or improvement of nerve-related discomfort conditions. Compared with the prior art, a chronic constraint stress (CRS)-induced puberty mouse anxiety model is constructed, functional evaluation is performed by behavioral experiments such as an open field experiment, a bead embedding experiment and a tail suspension experiment, and results show that the milk-derived extracellular vesicles remarkably improve mouse anxiety-like behaviors; it is shown that the milk-derived extracellular vesicles can significantly improve the chronic bound stress (CRS) induced adolescent mouse anxiety-like behavior by reshaping the intestinal-brain axis function, and the anxiety susceptibility of adolescents can be significantly reduced by supplementing the milk-derived extracellular vesicles to teenagers; meanwhile, a D-gal induced mouse cognitive impairment model is constructed, and the result shows that mucosa inflammation can be inhibited by reducing generation of proinflammatory cytokines, so that intestinal homeostasis is maintained, and cognitive impairment is relieved.

The present application relates to the technical field of biological experiment apparatus, disclose a kind of multifunctional mouse stress model establishment device, comprising: box frame and feeding mechanism, restraint mechanism, illumination mechanism, temperature control mechanism, acupuncture mechanism and control center;Box frame is by mutually perpendicular connection crossbeam, stringer and vertical beam, horizontal placement base plate is connected between vertical beam, the periphery of base plate is provided with inner layer breathable baffle, and inner layer breathable baffle constitutes mouse feeding space with base plate;Mouse is restrained by restraint mechanism and carries out restraint stress experiment, mouse is carried out by illumination mechanism and carries out high light stress experiment, mouse is carried out by temperature control mechanism and carries out cold and hot stress experiment, mouse is carried out by acupuncture mechanism and carries out foot bottom acupuncture and electric shock stress experiment.The device of the present application integrates the function of a variety of stress stimulation experiments, while realizing the components or mechanisms of each stress function can be coordinated with each other, can also be independent, facilitate simultaneously experiment one or multiple stress stimulation, wide application range.